DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ corbett-2007-1 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66850X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions imex curation corbett-2007-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66849X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions imex curation corbett-2007-3 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66851X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions imex curation corbett-2007-4 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66853X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions imex curation corbett-2007-5 ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66854X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions imex curation corbett-2007-6 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66852X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions imex curation top6b_metma Type 2 DNA topoisomerase 6 subunit B top6B Type II DNA topoisomerase VI subunit B MM_2417 protein protein metma Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) METPIAEELAKKQKSISVAEFFEKNRQILGFDSAPRSLITTVKEAVDNALDACEEAGILPDILVQVERTGPDYVTVIIEDNGPGIVREQIPKVFAKLLYGSRFHALKQSRGQQGIGISAAVLYAQMTAGRHTKILSKTSPTAPAHYYELMINTSTNEPDILVDEVRDWFRPHGTQIELEMRAAYVKGRRQSIYEYLKATAIVNPHARITLIDPDGNEEVFERATDKMPEPAEEILPHPEGIELGTLMKMLHYTERQKLAPFLRYSFCKIGLLTAEEICKAAGLDPEIDPHALGRHEARKLIEAFEKVKIMAPPTDCLSPIGEDLIYRGLEKETTVDFIATSTRKPAVYSGNPFVVEVGMAYGGNLPKEEKISIMRFANRVPLLYQQGGCVTTHAVEDIKWKQYGLNQPGGGIPVGPVILLIHVASINVPFTSESKDAIADIPVIKEEIDLAIKEVARKLKHYLSKQSNLKKRREKEIIITKVLPKLAAKVAHVLEKDVPDINPVVAKIMGNLLVHRVIKNNGDGTVDVAIKVKNFGTSAYSFRVHEMLPCKVSGAKPEPKVVTMGNDYDYVWDISASAGSSKVLSYKIESASEEELQKLPQLIVEGIEEELVTGAKAFKGV 7D2FEC4F2FA68AD6 top6a_metma Type 2 DNA topoisomerase 6 subunit A top6A Type II DNA topoisomerase VI subunit A MM_2418 protein protein metma Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) MEGEKGSKTRKGDALAREKLLEIAEKIYNQFEEEVVPSVSLPSRTKANLEYSDESDVWVYGDRESERSAKTVKGAFQLLKTTYATDFLINEHLARNRGSTLRELYYISEGWDYAKFKEQGESDRLIEDLEILTSLQREYFHMRPEEDGATMFGPIEITEQTKRGERNIHCQKDVGEGGYQIPFNVENIEFQKHDASMIIAIETGGMYARLMENGFDEAYNAILVHLKGQPARSTRRIIKRMNEELGIPVAVFTDGDPWSYRIYASVAYGAIKSAHLSEFMATPAAKFLGLQPSDIVEYELSTDKLTEQDVSALRSELSDPRFESDYWKEQIQLQLDIGKKAEQQAFAGKGLDFVTEVYLPNRLKEMGMI CF7FA9AC6F4936CA top6a_sacsh Type 2 DNA topoisomerase 6 subunit A top6A Type II DNA topoisomerase VI subunit A protein protein sulsh Sulfolobus shibatae MSSEFISKVDKEARRKAANILRDKFLNLVEQLKKGEPLVMEIPMRTLSNAIYDEKRKLLLLGEKKLRRNFLDLNEAKRFMQTVLMASIIYDALVSDEYPTIRDLYYRGKHSLLLKSIEGNKIVSEENTWDEQKESDSVIVDIEVFTSLLREEMLILSKEKGKVVGNLRIRSGNDVIDLSKTGHGAYAIEPTPDLIDFIDVDAEFVLVVEKDAVFQQLHRAGFWKQYKSILITSAGQPDRATRRFVRRLNEELKLPVYILTDADPYGWYIFSVFRIGSISLSYESERLATPDAKFLGVSMGDIFGNSRKKPYLSEAERKNYIIKAKDADIKRAEEIKNYEWFKTKAWQEEINTFLQRKAKLEIEAMASKGLKFLAFQYIPEKITNKDYIA top6b_sacsh Type 2 DNA topoisomerase 6 subunit B top6B Type II DNA topoisomerase VI subunit B protein protein sulsh Sulfolobus shibatae MSAKEKFTSLSPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDVHGILPNIKITIDLIDDARQIYKVNVVDNGIGIPPQEVPNAFGRVLYSSKYVNRQTRGMYGLGVKAAVLYSQMHQDKPIEIETSPVNSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIPGDWPKAKSRIYEYIKRTYIITPYAEFIFKDPEGNVTYYPRLTNKIPKPPQEVKPHPYGVDREEIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAGLKPNKKVKNLTEEEITRLVETFKKDEDFRSPSADSLSVIGEDLIELGLKKIFNPDFAASITRKPKAYQGHPFIVEAGVAFGGSIPVGEEPIVLRYANKIPLIYDEKSDVIWKVVEELDWKRYGIESDQYQMVVMVHLCSTKIPYKSAGKESIAEVENIEKEIKNALMEVARKLKQYLSEKRKEQEAKKKLLAYLKYIPEVSRSLATFLASGNKELVSKYQNEISEGLFKLISKKLDLINIEEYRKVYRVDSE top6b-top6a-1 3158314 N/A 3158320 unspecified role unspecified role bait bait His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 1-511 binding region 1-511 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3158321 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false top6a-top6b-1 3158315 N/A 3158320 unspecified role unspecified role neutral component neutral component 1-511 binding region 1-511 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3158321 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false top6a-top6b-2 3158316 N/A 3158322 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3158323 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false top6a-top6b-3 3158317 N/A 3158322 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3158323 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false top6b-3 3158318 N/A 3158323 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction false false false top6b-top6a-2 3158319 N/A 3158322 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3158323 unspecified role unspecified role bait bait His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false