DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ couture-2007-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. DIP original experiment: DIP-68384X Nature structural & molecular biology 2007 IMEx Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. Only protein-protein interactions imex curation couture-2007-2 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. DIP original experiment: DIP-68388X Nature structural & molecular biology 2007 IMEx Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. Only protein-protein interactions imex curation couture-2007-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. DIP original experiment: DIP-68385X Nature structural & molecular biology 2007 IMEx Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. Only protein-protein interactions imex curation couture-2007-4 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. DIP original experiment: DIP-68387X Nature structural & molecular biology 2007 IMEx Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. Only protein-protein interactions imex curation couture-2007-5 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. DIP original experiment: DIP-68386X Nature structural & molecular biology 2007 IMEx Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Couture JF., Collazo E., Ortiz-Tello PA., Brunzelle JS., Trievel RC. Only protein-protein interactions imex curation h3k9-1 H3K9 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKA o75164-1 Lysine-specific demethylase 4A [histone H3]-trimethyl-L-lysine(36) demethylase 4A [histone H3]-trimethyl-L-lysine(9) demethylase 4A KDM4A JHDM3A JMJD2 JMJD2A KIAA0677 JmjC domain-containing histone demethylation protein 3A Jumonji domain-containing protein 2A protein protein human Homo sapiens Human MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRASYDDIDDLVIPAPIQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTLYEKHVDEWNIGRLRTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYSVPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFNHGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEAAEFLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKTSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDLSSEQYEMTECPAALAPVRPTHSSVRQVEDGLTFPDYSDSTEVKFEELKNVKLEEEDEEEEQAAAALDLSVNPASVGGRLVFSGSKKKSSSSLGSGSSRDSISSDSETSEPLSCRAQGQTGVLTVHSYAKGDGRVTVGEPCTRKKGSAARSFSERELAEVADEYMFSLEENKKSKGRRQPLSKLPRHHPLVLQECVSDDETSEQLTPEEEAEETEAWAKPLSQLWQNRPPNFEAEKEFNETMAQQAPHCAVCMIFQTYHQVEFGGFNQNCGNASDLAPQKQRTKPLIPEMCFTSTGCSTDINLSTPYLEEDGTSILVSCKKCSVRVHASCYGVPPAKASEDWMCSRCSANALEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCIFCKKRRKRTAGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALQSITAGQKVISKHKNGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVSQDCLQFGPPAEGEVVQVRWTDGQVYGAKFVASHPIQMYQVEFEDGSQLVVKRDDVYTLDEELPKRVKSRLSVASDMRFNEIFTEKEVKQEKKRQRVINSRYREDYIEPALYRAIME kdm4d_human Lysine-specific demethylase 4D KDM4D JHDM3D JMJD2D JmjC domain-containing histone demethylation protein 3D Jumonji domain-containing protein 2D [histone H3]-trimethyl-L-lysine(9) demethylase 4D protein protein human Homo sapiens Human METMKSKANCAQNPNCNIMIFHPTKEEFNDFDKYIAYMESQGAHRAGLAKIIPPKEWKARETYDNISEILIATPLQQVASGRAGVFTQYHKKKKAMTVGEYRHLANSKKYQTPPHQNFEDLERKYWKNRIYNSPIYGADISGSLFDENTKQWNLGHLGTIQDLLEKECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHLGEPKTWYVVPPEHGQRLERLARELFPGSSRGCGAFLRHKVALISPTVLKENGIPFNRITQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDYGKMASQCSCGEARVTFSMDAFVRILQPERYDLWKRGQDRAVVDHMEPRVPASQELSTQKEVQLPRRAALGLRQLPSHWARHSPWPMAARSGTRCHTLVCSSLPRRSAVSGTATQPRAAAVHSSKKPSSTPSSTPGPSAQIIHPSNGRRGRGRPPQKLRAQELTLQTPAKRPLLAGTTCTASGPEPEPLPEDGALMDKPVPLSPGLQHPVKASGCSWAPVP h3k36-1 H3K36 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) RKSAPATGGVKKPHRY h3k9-kdm4a-5 3157945 N/A 3157950 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3157951 unspecified role unspecified role neutral component neutral component 1-350 binding region 1-350 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3k9-kdm4d-1 3157946 N/A 3157952 enzyme enzyme neutral component neutral component 3-354 binding region 3-354 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q6B0I6:p.Ala292Ser A291S mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3157950 enzyme target enzyme target substrate substrate neutral component neutral component K9me3 methylation site K9me3 methylation site n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false K9me2 methylation site K9me2 methylation site n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) demethylation demethylation reaction demethylation false false false h3k36-kdm4a-3 3157947 N/A 3157953 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3157951 unspecified role unspecified role neutral component neutral component 1-350 binding region 1-350 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3k36-kdm4a-4 3157948 N/A 3157953 enzyme target enzyme target substrate substrate neutral component neutral component H3K36 methylation site H3K36 methylation site n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3157951 enzyme enzyme neutral component neutral component 1-350 binding region 1-350 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli demethylation demethylation reaction demethylation false false false h3k9-kdm4a-6 3157949 N/A 3157950 enzyme target enzyme target substrate substrate neutral component neutral component h3k9-1:p.Gly12Pro G12P mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false K9me2 methylation site K9me2 methylation site n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false K9me3 methylation site K9me3 methylation site n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false h3k9-1:p.Ala7Arg A7R mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3157951 enzyme enzyme neutral component neutral component O75164-1:p.Ser288Ala S288A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false 1-350 binding region 1-350 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli demethylation demethylation reaction demethylation false false false