DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ davies-2007-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Davies OR., Pellegrini L. DIP original experiment: DIP-255050X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-2 in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255056X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-3 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255052X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-4 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255054X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255060X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-6 ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255057X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-7 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Davies OR., Pellegrini L. DIP original experiment: DIP-255051X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-8 in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255055X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-9 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255059X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-10 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Davies OR., Pellegrini L. DIP original experiment: DIP-255053X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation davies-2007-11 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Davies OR., Pellegrini L. DIP original experiment: DIP-255058X Nature structural & molecular biology 2007 IMEx Interaction with the BRCA2 C terminus protects RAD51-DNA filaments from disassembly by BRC repeats. Davies OR., Pellegrini L. Only protein-protein interactions imex curation q06609-1 DNA repair protein RAD51 homolog 1 RAD51 homolog A RAD51 RAD51A RECA protein protein human Homo sapiens Human MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD brca2_human Breast cancer type 2 susceptibility protein BRCA2 FANCD1 FACD Fanconi anemia group D1 protein protein protein human Homo sapiens Human MPIGSKERPTFFEIFKTRCNKADLGPISLNWFEELSSEAPPYNSEPAEESEHKNNNYEPNLFKTPQRKPSYNQLASTPIIFKEQGLTLPLYQSPVKELDKFKLDLGRNVPNSRHKSLRTVKTKMDQADDVSCPLLNSCLSESPVVLQCTHVTPQRDKSVVCGSLFHTPKFVKGRQTPKHISESLGAEVDPDMSWSSSLATPPTLSSTVLIVRNEEASETVFPHDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKDHIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADECEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVVPSLACEWSQLTLSGLNGAQMEKIPLLHISSCDQNISEKDLLDTENKRKKDFLTSENSLPRISSLPKSEKPLNEETVVNKRDEEQHLESHTDCILAVKQAISGTSPVASSFQGIKKSIFRIRESPKETFNASFSGHMTDPNFKKETEASESGLEIHTVCSQKEDSLCPNLIDNGSWPATTTQNSVALKNAGLISTLKKKTNKFIYAIHDETSYKGKKIPKDQKSELINCSAQFEANAFEAPLTFANADSGLLHSSVKRSCSQNDSEEPTLSLTSSFGTILRKCSRNETCSNNTVISQDLDYKEAKCNKEKLQLFITPEADSLSCLQEGQCENDPKSKKVSDIKEEVLAAACHPVQHSKVEYSDTDFQSQKSLLYDHENASTLILTPTSKDVLSNLVMISRGKESYKMSDKLKGNNYESDVELTKNIPMEKNQDVCALNENYKNVELLPPEKYMRVASPSRKVQFNQNTNLRVIQKNQEETTSISKITVNPDSEELFSDNENNFVFQVANERNNLALGNTKELHETDLTCVNEPIFKNSTMVLYGDTGDKQATQVSIKKDLVYVLAEENKNSVKQHIKMTLGQDLKSDISLNIDKIPEKNNDYMNKWAGLLGPISNHSFGGSFRTASNKEIKLSEHNIKKSKMFFKDIEEQYPTSLACVEIVNTLALDNQKKLSKPQSINTVSAHLQSSVVVSDCKNSHITPQMLFSKQDFNSNHNLTPSQKAEITELSTILEESGSQFEFTQFRKPSYILQKSTFEVPENQMTILKTTSEECRDADLHVIMNAPSIGQVDSSKQFEGTVEIKRKFAGLLKNDCNKSASGYLTDENEVGFRGFYSAHGTKLNVSTEALQKAVKLFSDIENISEETSAEVHPISLSSSKCHDSVVSMFKIENHNDKTVSEKNNKCQLILQNNIEMTTGTFVEEITENYKRNTENEDNKYTAASRNSHNLEFDGSDSSKNDTVCIHKDETDLLFTDQHNICLKLSGQFMKEGNTQIKEDLSDLTFLEVAKAQEACHGNTSNKEQLTATKTEQNIKDFETSDTFFQTASGKNISVAKESFNKIVNFFDQKPEELHNFSLNSELHSDIRKNKMDILSYEETDIVKHKILKESVPVGTGNQLVTFQGQPERDEKIKEPTLLGFHTASGKKVKIAKESLDKVKNLFDEKEQGTSEITSFSHQWAKTLKYREACKDLELACETIEITAAPKCKEMQNSLNNDKNLVSIETVVPPKLLSDNLCRQTENLKTSKSIFLKVKVHENVEKETAKSPATCYTNQSPYSVIENSALAFYTSCSRKTSVSQTSLLEAKKWLREGIFDGQPERINTADYVGNYLYENNSNSTIAENDKNHLSEKQDTYLSNSSMSNSYSYHSDEVYNDSGYLSKNKLDSGIEPVLKNVEDQKNTSFSKVISNVKDANAYPQTVNEDICVEELVTSSSPCKNKNAAIKLSISNSNNFEVGPPAFRIASGKIVCVSHETIKKVKDIFTDSFSKVIKENNENKSKICQTKIMAGCYEALDDSEDILHNSLDNDECSTHSHKVFADIQSEEILQHNQNMSGLEKVSKISPCDVSLETSDICKCSIGKLHKSVSSANTCGIFSTASGKSVQVSDASLQNARQVFSEIEDSTKQVFSKVLFKSNEHSDQLTREENTAIRTPEHLISQKGFSYNVVNSSAFSGFSTASGKQVSILESSLHKVKGVLEEFDLIRTEHSLHYSPTSRQNVSKILPRVDKRNPEHCVNSEMEKTCSKEFKLSNNLNVEGGSSENNHSIKVSPYLSQFQQDKQQLVLGTKVSLVENIHVLGKEQASPKNVKMEIGKTETFSDVPVKTNIEVCSTYSKDSENYFETEAVEIAKAFMEDDELTDSKLPSHATHSLFTCPENEEMVLSNSRIGKRRGEPLILVGEPSIKRNLLNEFDRIIENQEKSLKASKSTPDGTIKDRRLFMHHVSLEPITCVPFRTTKERQEIQNPNFTAPGQEFLSKSHLYEHLTLEKSSSNLAVSGHPFYQVSATRNEKMRHLITTGRPTKVFVPPFKTKSHFHRVEQCVRNINLEENRQKQNIDGHGSDDSKNKINDNEIHQFNKNNSNQAAAVTFTKCEEEPLDLITSLQNARDIQDMRIKKKQRQRVFPQPGSLYLAKTSTLPRISLKAAVGGQVPSACSHKQLYTYGVSKHCIKINSKNAESFQFHTEDYFGKESLWTGKGIQLADGGWLIPSNDGKAGKEEFYRALCDTPGVDPKLISRIWVYNHYRWIIWKLAAMECAFPKEFANRCLSPERVLLQLKYRYDTEIDRSRRSAIKKIMERDDTAAKTLVLCVSDIISLSANISETSSNKTSSADTQKVAIIELTDGWYAVKAQLDPPLLAVLKNGRLTVGQKIILHGAELVGSPDACTPLEAPESLMLKISANSTRPARWYTKLGFFPDPRPFPLPLSSLFSDGGNVGCVDVIIQRAYPIQWMEKTSSGLYIFRNEREEEKEAAKYVEAQQKRLEALFTKIQEEFEEHEENTTKPYLPSRALTRQQVRALQDGAELYEAVKNAADPAYLEGYFSEEQLRALNNHRQMLNDKKQAQIQLEIRKAMESAEQKEQGLSRDVTTVWKLRIVSYSKKEKDSVILSIWRPSSDLYSLLTEGKRYRIYHLATSKSKSKSERANIQLAATKKTQYQQLPVSDEILFQIYQPREPLHFSKFLDPDFQPSCSEVDLIGFVVSVVKKTGLAPFVYLSDECYNLLAIKFWIDLNEDIIKPHMLIAASNLQWRPESKSGLLTLFAGDFSVFSASPKEGHFQETFNKMKNTVENIDILCNEAENKLMHILHANDPKWSTPTKDCTSGPYTAQIIPGTGNKLLMSSPNCEIYYQSPLSLCMAKRKSVSTPVSAQMTSKSCKGEKEIDDQKNCKKRRALDFLSRLPLPPPVSPICTFVSPAAQKAFQPPRSCGTKYETPIKKKELNSPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSESTRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI brca2-rad51-27 3156489 N/A 3156500 unspecified role unspecified role prey prey Region 80-339 Region 80-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role bait bait Region 3270-3305 Region 3270-3305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pS3291 pS3291 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false brca2-rad51-28 3156490 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 18-339 Region 18-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role unspecified role unspecified role Region 3270-3305 Region 3270-3305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false brca2-rad51-29 3156491 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 18-339 Region 18-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role unspecified role unspecified role Region 1514-1548 Region 1514-1548 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false rad51-5 3156492 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 18-339 Region 18-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false brca2-rad51-30 3156493 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 80-339 Region 80-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role unspecified role unspecified role Region 1514-1548 Region 1514-1548 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false rad51-6 3156494 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 18-339 Region 18-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false brca2-rad51-31 3156495 N/A 3156500 unspecified role unspecified role prey prey Region 80-339 Region 80-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role bait bait Region 1514-1548 Region 1514-1548 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false brca2-rad51-32 3156496 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 18-339 Region 18-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role unspecified role unspecified role Region 1514-1548 Region 1514-1548 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false rad51-7 3156497 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 80-339 Region 80-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false brca2-rad51-33 3156498 N/A 3156500 unspecified role unspecified role unspecified role unspecified role Region 18-339 Region 18-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role unspecified role unspecified role Region 3270-3305 Region 3270-3305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false brca2-rad51-34 3156499 N/A 3156500 unspecified role unspecified role prey prey Region 80-339 Region 80-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q06609-1:p.Phe86Glu F86E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156501 unspecified role unspecified role bait bait Region 3270-3305 Region 3270-3305 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false