DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhao-2007-10 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261477X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions imex curation zhao-2007-11 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261481X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions imex curation zhao-2007-12 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261480X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions imex curation zhao-2007-13 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261482X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions imex curation zhao-2007-14 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261478X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions imex curation zhao-2007-15 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261479X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions imex curation ngly1_mouse Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Ngly1 N-glycanase 1 Peptide:N-glycanase protein protein mouse Mus musculus Mouse MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL tera_mouse Transitional endoplasmic reticulum ATPase Vcp 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein mouse Mus musculus Mouse MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG ngly1-vcp-3 3156247 N/A 3156253 unspecified role unspecified role unspecified role unspecified role Region 12-111 Region 12-111 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156254 unspecified role unspecified role unspecified role unspecified role Region 801-806 Region 801-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false ngly1-vcp-4 3156248 N/A 3156253 unspecified role unspecified role unspecified role unspecified role Q9JI78:p.Arg55Glu R55E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156254 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ngly1-vcp-5 3156249 N/A 3156253 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-130 Region 1-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156254 unspecified role unspecified role prey prey Q01853:p.Tyr805Glu Y805E/F mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q01853:p.Tyr805Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ngly1-vcp-6 3156250 N/A 3156253 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3156254 unspecified role unspecified role unspecified role unspecified role Region 767-806 Region 767-806 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ngly1-vcp-7 3156251 N/A 3156253 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3156254 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ngly1-vcp-8 3156252 N/A 3156253 unspecified role unspecified role unspecified role unspecified role Q9JI78:p.Arg55Glu R55E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9JI78:p.Asn58Asp N58D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3156254 unspecified role unspecified role unspecified role unspecified role Region 797-806 Region 797-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pY805 pY805 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false