DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ rubio-2007-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Rubio MA., Pastar I., Gaston KW., Ragone FL., Janzen CJ., Cross GA., Papavasiliou FN., Alfonzo JD. DIP original experiment: DIP-261458X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Rubio MA., Pastar I., Gaston KW., Ragone FL., Janzen CJ., Cross GA., Papavasiliou FN., Alfonzo JD. Only protein-protein interactions imex curation rubio-2007-2 ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Rubio MA., Pastar I., Gaston KW., Ragone FL., Janzen CJ., Cross GA., Papavasiliou FN., Alfonzo JD. DIP original experiment: DIP-261459X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Rubio MA., Pastar I., Gaston KW., Ragone FL., Janzen CJ., Cross GA., Papavasiliou FN., Alfonzo JD. Only protein-protein interactions imex curation rubio-2007-3 ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Rubio MA., Pastar I., Gaston KW., Ragone FL., Janzen CJ., Cross GA., Papavasiliou FN., Alfonzo JD. DIP original experiment: DIP-261460X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Rubio MA., Pastar I., Gaston KW., Ragone FL., Janzen CJ., Cross GA., Papavasiliou FN., Alfonzo JD. Only protein-protein interactions imex curation q381q7_tryb2 Tb11.01.6930 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MEEVVVPEEPPKLVSALATYVQQERLCTMFLSIANKLLPLKPHACHLKRIRRSSATRVATAPMDGFAGGVICDKRDSSVATSTISDGCERNSAALGTPAAENSHILELLLSVGGPVDSSALKELESAADTTVAVHRVWVPDRAPRSSAEEWTKWCQIWPFATPKPRAPTQLSECEMGSIQRIFRTVVMPLAKRLRTDETLGIAAVLVDPSDGYRVLVSSGEEHALKRGNSAACLGYVSNSGCRKSNRVVLDHPVTFVLKEVTRKQCKDREVEGDASYLANGMDMFVSHEPCVMCSMALVHSRVRRVFYCFPNPVHGGLGSTVSIHAIQELNHHFRVFRCDSRWLSDPEGVSSDHDNPYWEDLTVP 79899E5574864EE6 q57w17_tryb2 Tb927.8.4180 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVQDTGKDTNLKGTAEANESVVYCDVFMQAALKEATCALEEGEVPVGCVLVKADSSTAAQAQAGDDLALQKLIVARGRNATNRKGHALAHAEFVAVEELLRQATAGTSENIGGGGNCGAVSQDLADYVLYVVVEPCIMCAAMLLYNRVRKVYFGCTNPRFGGNGTVLSVHNSYKGCSGEDAALIGYESCGGYRAEEAVVLLQQFYRRENTNAPLGKRKRKDLSVV D57803D6E83AEDF6 tbadat2-tbadat3-1 3156038 N/A 3156041 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3156042 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tbadat2-tbadat3-2 3156039 N/A 3156041 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3156042 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false tbadat3-tbadat2-1 3156040 N/A 3156041 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3156042 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false