DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ morales-2007-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. DIP original experiment: DIP-66885X Structure (London, England : 1993) 2007 IMEx NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. Only protein-protein interactions imex curation morales-2007-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. DIP original experiment: DIP-66886X Structure (London, England : 1993) 2007 IMEx NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. Only protein-protein interactions imex curation morales-2007-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. DIP original experiment: DIP-66887X Structure (London, England : 1993) 2007 IMEx NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. Only protein-protein interactions imex curation pypeptide Latent membrane protein 2 [SNEEPPPPYEDPYWGN] peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SNEEPPPPYEDPYWGN itch_mouse E3 ubiquitin-protein ligase Itchy Itch HECT-type E3 ubiquitin transferase Itchy homolog protein protein mouse Mus musculus Mouse MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE py peptide Latent membrane protein 2 [EEPPPPYED] peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EEPPPPYED itch-itch-1 3155187 N/A 3155190 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3155190 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3155191 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3155191 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false itch-itch-2 3155188 N/A 3155190 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3155190 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3155191 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3155191 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false itch-itch-3 3155189 N/A 3155192 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3155192 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3155191 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3155191 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false