IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hsieh-2007-1 yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Hsieh EJ., Gin P., Gulmezian M., Tran UC., Saiki R., Marbois BN., Clarke CF. Purified mitochondria were solubilized with digitonin and subject to 100,000 × g centrifugation. then to 2-dimensional Blue Native gel electrophoresis. cathy@chem.ucla.edu Archives of biochemistry and biophysics 2007 imex curation Only protein-protein interactions hsieh-2007-2 yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hsieh EJ., Gin P., Gulmezian M., Tran UC., Saiki R., Marbois BN., Clarke CF. Purified mitochondria were solubilized with digitonin and subject to 100,000 × g centrifugation. then to 2-dimensional Blue Native gel electrophoresis. cathy@chem.ucla.edu Archives of biochemistry and biophysics 2007 imex curation Only protein-protein interactions coq9_yeast Ubiquinone biosynthesis protein COQ9, mitochondrial YLR201C COQ9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLCRNTARTGCKFFRLYHSNPIEHVKPIHIKPLTYGKESPQYKVLSLALQKFVPEHGFSERSIVESLNELGYPSSMISSIGAPNSPSFFHSSTAVMELIKFQLVDKRYRLTEGINPDVTPQYKLPSLEHLLLKRLEMDKPIGGHLSELMSQLAIPSAFLFETAIPELHRLSDDMIYFSNEKDHHDSAWYAKRLAVSSTYIGSKLFMAQDKSHNYKETFTFAKDKLHRVMRLGEYYNNTEEFAWYTLMSTVNLIKSQLVRG coq8_yeast Atypical kinase COQ8, mitochondrial COQ8 ABC1 Activity of bc1 complex protein 1 Coenzyme Q protein 8 Ubiquinone biosynthesis protein COQ8 YGL119W protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTNMVKLRNLRRLYCSSRLLRTIQNGRISSVSSISLSKKYTTKSAKEGEENVERKHEEEKKDTLKSSSVPTSRISRLFHYGSLAAGVGMNAAAKGISEVAKGNSPTWKSLILSDSNIDRITNKFSKMRGVALKIGQLLSFQDEKVLPKELYEILSRVQNSANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQYPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKFEALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCLEEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDRKGAYEMSVQLGYLTGLESQSMKDAHVDSVLTLGEPFRGDVDKSFDFKDQTVSDRIRGNIGLMLNERLCPPPEETYSLHRKFSGIFLLCARMGASVHCAKLFKEIFAYKV coq5_yeast 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial COQ5 DBI56 YML110C YM8339.09C Ubiquinone biosynthesis methyltransferase COQ5 2-hexaprenyl-6-methoxy-1,4-benzoquinol methyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLISSRIVRSSLVNVPLRLSRCFTQAHRACKEEEVNSPLSSAAEQPEQKYTHFGSKTVLKSTKQKLVGDVFSSVANRYDLMNDVMSLGIHRLWKDHFINKLDAGKRPNSTTPLNFIDVAGGSGDIAFGLLDHAESKFGDTESTMDIVDINPDMLKEGEKRAMEQGKYFKDPRVRFLVSNGEKLEEIDSDSKDIYTVSFGIRNFTDIQKGLNTAYRVLKPGGIFYCLEFSKIENPLMDFAYQQWAKVLPVMGSMIANDYDSYQYLVESIERFPDQETFKSMIEKAGFKSAGYESLTFGICAIHWGIKV coq4_yeast Ubiquinone biosynthesis protein COQ4, mitochondrial COQ4 YDR204W YD8142.01 Coenzyme Q biosynthesis protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRLSLLRSTATLPVKCQRRGLILPAAAMYTLGSLIFGKEARLADAMERGELHNKNVDYAKEAEERTELRIRALANTRPMEPRYNGHVPLHRYEKLLLFAISGWNSFFHPEDGYNIVQLGEATALPVFLENLKQTMLSDSSGRRILKEQPNITTEILHMDKLAKLPHNTFGYVYYQWLKRENVSPDTRAPVKFIDDPMHAYIFKRYRQCHDFYHAITNMPIIIEGEITIKALEGANLGVPMAILGGILAPLRLKKVQRKRLYNIYLPWAVRTGLSCKPLINVYWEEMLEKDVTALRKELKITLPPDLRTMRKERAALRKEIDAKYNSQKRATTPA coq3_yeast Ubiquinone biosynthesis O-methyltransferase, mitochondrial COQ3 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase 3-demethylubiquinol 3-O-methyltransferase 3-demethylubiquinone-6 3-O-methyltransferase Hexaprenyldihydroxybenzoate methyltransferase Polyprenyldihydroxybenzoate methyltransferase HRF316 YOL096C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLLRSRFLKVIHVRKQLSACSRFAIQTQTRCKSTDASEDEVKHFQELAPTWWDTDGSQRILHKMNLTRLDFVQRTVRNQVKIQNPEIFVPGFNYKEFLPEYVCDNIQREMQESIETNLDKRPEVSVLDVGCGGGILSESLARLKWVKNVQGIDLTRDCIMVAKEHAKKDPMLEGKINYECKALEDVTGQFDIITCMEMLEHVDMPSEILRHCWSRLNPEKGILFLSTINRDLISWFTTIFMGENVLKIVPKGTHHLSKYINSKEILAWFNDNYSGQFRLLDLKGTMYLPYQGWVEHDCSDVGNYFMAIQRLN coq6_yeast Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial COQ6 Ubiquinone C4-deaminase G9165 YGR255C protein protein yeast Saccharomyces cerevisiae Baker's yeast MFFSKVMLTRRILVRGLATAKSSAPKLTDVLIVGGGPAGLTLAASIKNSPQLKDLKTTLVDMVDLKDKLSDFYNSPPDYFTNRIVSVTPRSIHFLENNAGATLMHDRIQSYDGLYVTDGCSKATLDLARDSMLCMIEIINIQASLYNRISQYDSKKDSIDIIDNTKVVNIKHSDPNDPLSWPLVTLSNGEVYKTRLLVGADGFNSPTRRFSQIPSRGWMYNAYGVVASMKLEYPPFKLRGWQRFLPTGPIAHLPMPENNATLVWSSSERLSRLLLSLPPESFTALINAAFVLEDADMNYYYRTLEDGSMDTDKLIEDIKFRTEEIYATLKDESDIDEIYPPRVVSIIDKTRARFPLKLTHADRYCTDRVALVGDAAHTTHPLAGQGLNMGQTDVHGLVYALEKAMERGLDIGSSLSLEPFWAERYPSNNVLLGMADKLFKLYHTNFPPVVALRTFGLNLTNKIGPVKNMIIDTLGGNEK coq7_yeast 5-demethoxyubiquinone hydroxylase, mitochondrial COQ7 CAT5 Catabolite repression protein 5 Ubiquinone biosynthesis monooxygenase COQ7 O3284 YOR3284C YOR125C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSRVSVFKPASRGFSVLSSLKITEHTSAKHTEKPEHAPKCQNLSDAQAAFLDRVIRVDQAGELGADYIYAGQYFVLAHRYPHLKPVLKHIWDQEIHHHNTFNNLQLKRRVRPSLLTPLWKAGAFAMGAGTALISPEAAMACTEAVETVIGGHYNGQLRNLANQFNLERTDGTKGPSEEIKSLTSTIQQFRDDELEHLDTAIKHDSYMAVPYTVITEGIKTICRVAIWSAERI 2686F44C5F17EFE5 coq3-coq4-1 3037983 N/A 3037985 unspecified role unspecified role neutral component neutral component N/A 3037986 unspecified role unspecified role neutral component neutral component N/A 3037987 unspecified role unspecified role neutral component neutral component N/A 3037988 unspecified role unspecified role neutral component neutral component N/A 3037989 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 4B coq3-coq4-2 3037984 N/A 3037985 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3037987 unspecified role unspecified role prey prey N/A 3037988 unspecified role unspecified role prey prey N/A 3037990 unspecified role unspecified role prey prey N/A 3037991 unspecified role unspecified role prey prey association association false false false Fig. 5