DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ring-2007-1 in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Ring AM., Leng Q., Rinehart J., Wilson FH., Kahle KT., Hebert SC., Lifton RP. DIP original experiment: DIP-261149X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx An SGK1 site in WNK4 regulates Na+ channel and K+ channel activity and has implications for aldosterone signaling and K+ homeostasis. Ring AM., Leng Q., Rinehart J., Wilson FH., Kahle KT., Hebert SC., Lifton RP. Only protein-protein interactions imex curation ring-2007-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ring AM., Leng Q., Rinehart J., Wilson FH., Kahle KT., Hebert SC., Lifton RP. DIP original experiment: DIP-261150X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx An SGK1 site in WNK4 regulates Na+ channel and K+ channel activity and has implications for aldosterone signaling and K+ homeostasis. Ring AM., Leng Q., Rinehart J., Wilson FH., Kahle KT., Hebert SC., Lifton RP. Only protein-protein interactions imex curation wnk4_mouse Serine/threonine-protein kinase WNK4 Wnk4 Prkwnk4 Protein kinase with no lysine 4 Protein kinase lysine-deficient 4 protein protein mouse Mus musculus Mouse MLAPRNTETGVPMSQTEADLALRPSPALTSTGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPTRSPPSSSKEPPEGTWMGAAPVKAVDSACPELTGSSGGPGSREPPRVPDAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSLAPGPPSAFPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGLPSSPAESHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPPEAAEDALSPQEEPAALPALPGPPNAEPQRSISPEQRSWAAFSTSPSSPGTPLSPGAPFSPGTPPVFPCPIFPITSPSCYPCPFSQVSSNPYPQAPSSLLPLSSSASQVPLPSSSLPISAPLPFSPSYPQDPLSPTSLPVCPSPPSLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPLSLASCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPSPPLTSESSDTEDSAAGGPETREALAESDRAAEGLGVAVDDEKDEGKEPLLGGSSPILSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDIGRM sgk1_human Serine/threonine-protein kinase Sgk1 SGK1 SGK Serum/glucocorticoid-regulated kinase 1 protein protein human Homo sapiens Human MTVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL sgk1-wnk4-1 3153739 N/A 3153741 enzyme target enzyme target substrate substrate unspecified role unspecified role Q80UE6:p.Ser1169Ala S1169A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 1127-1179 Region 1127-1179 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3153742 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false wnk4-sgk1-1 3153740 N/A 3153742 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3153741 unspecified role unspecified role bait bait Q80UE6:p.Ser1169Asp S1169D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q80UE6:p.Gln562Glu Q562E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false