IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ haug-2007-1 in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Haug M., Schepp CP., Kalbacher H., Dannecker GE., Holzer U. Interactions involving HSC70(Hsc73) have not been included as the main text and Methods sections are ambiguous as to the origins of this protein - human or bovine? DELFIA: Dissociation-enhanced lanthanide fluorescent immunoassay. Hsp70/Hsc70/DnaK and biotinylated peptide/protein mixtures were either added directly to plates, or plates coated with anti-Hsp70/Hsc70/DnaK monoclonal antibody. After washing biotinylated peptides were incubated with europium-labelled streptavidin and fluorescence was detected. ursula.holzer@med.uni‐tuebingen.de European journal of immunology 2007 imex curation Only protein-protein interactions dnak_ecoli Chaperone protein DnaK dnaK groP grpF seg b0014 Heat shock protein 70 Heat shock 70 kDa protein HSP70 JW0013 protein protein ecoli Escherichia coli (strain K12) MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK a0a0g4pyz0_human HLA-DRB1 protein protein human Homo sapiens Human MVCLRLPGGSCMAVLTVTLMVLSSPLALAGDTRPRFLEYSTGECYFFNGTERVRFLDRYFYNQEEYVRFDSDVGEYRAVTELGRPSAEYWNSQKDFLEDRRALVDTYCRHNYGVGESFTVQRRVHPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKTGVVSTGLIHNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWSARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS q0r326_human HLA-DRB1 HLA-DRB5 hCG_1998957 protein protein human Homo sapiens Human MVCLKLPGGSCMAALTVTLMVLSSPLALAGDTQPRFLKQDKFECHFFNGTERVRYLHRGIYNQEENVRFDSDVGEYRAVTELGRPVAESWNSQKDFLERRRAEVDTVCRHNYGVGESFTVQRRVHPEVTVYPAKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVMSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS a0a1v1igj9_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKFPGGSCMAALTVTLMVLSSPLALAGDTRPRFLEQVKHECHFFNGTERVRFLDRYFYHQEEYVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQKRAAVDTYCRHNYGVGESFTVQRRVYPEVTVYPAKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKTGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSLTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS d7rig0_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKLPGGSCMTALTVTLMVLSSPLALAGDTRPRFLWQLKFECHFFNGTERVRLLERCIYNQEESVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS hg2a_human HLA class II histocompatibility antigen gamma chain DHLAG HLA-DR antigens-associated invariant chain Ia antigen-associated invariant chain CD74 protein protein human Homo sapiens Human MHRRRSRSCREDQKPVMDDQRDLISNNEQLPMLGRRPGAPESKCSRGALYTGFSILVTLLLAGQATTAYFLYQQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKVLTKCQEEVSHIPAVHPGSFRPKCDENGNYLPLQCYGSIGYCWCVFPNGTEVPNTRSRGHHNCSESLELEDPSSGLGVTKQDLGPVPM tetx_clote Tetanus toxin tetX Tentoxylysin CTC_p60 protein protein Clostridium tetani (strain Massachusetts / E88) Clostridium tetani (strain Massachusetts / E88) MPITINNFRYSDPVNNDTIIMMEPPYCKGLDIYYKAFKITDRIWIVPERYEFGTKPEDFNPPSSLIEGASEYYDPNYLRTDSDKDRFLQTMVKLFNRIKNNVAGEALLDKIINAIPYLGNSYSLLDKFDTNSNSVSFNLLEQDPSGATTKSAMLTNLIIFGPGPVLNKNEVRGIVLRVDNKNYFPCRDGFGSIMQMAFCPEYVPTFDNVIENITSLTIGKSKYFQDPALLLMHELIHVLHGLYGMQVSSHEIIPSKQEIYMQHTYPISAEELFTFGGQDANLISIDIKNDLYEKTLNDYKAIANKLSQVTSCNDPNIDIDSYKQIYQQKYQFDKDSNGQYIVNEDKFQILYNSIMYGFTEIELGKKFNIKTRLSYFSMNHDPVKIPNLLDDTIYNDTEGFNIESKDLKSEYKGQNMRVNTNAFRNVDGSGLVSKLIGLCKKIIPPTNIRENLYNRTASLTDLGGELCIKIKNEDLTFIAEKNSFSEEPFQDEIVSYNTKNKPLNFNYSLDKIIVDYNLQSKITLPNDRTTPVTKGIPYAPEYKSNAASTIEIHNIDDNTIYQYLYAQKSPTTLQRITMTNSVDDALINSTKIYSYFPSVISKVNQGAQGILFLQWVRDIIDDFTNESSQKTTIDKISDVSTIVPYIGPALNIVKQGYEGNFIGALETTGVVLLLEYIPEITLPVIAALSIAESSTQKEKIIKTIDNFLEKRYEKWIEVYKLVKAKWLGTVNTQFQKRSYQMYRSLEYQVDAIKKIIDYEYKIYSGPDKEQIADEINNLKNKLEEKANKAMININIFMRESSRSFLVNQMINEAKKQLLEFDTQSKNILMQYIKANSKFIGITELKKLESKINKVFSTPIPFSYSKNLDCWVDNEEDIDVILKKSTILNLDINNDIISDISGFNSSVITYPDAQLVPGINGKAIHLVNNESSEVIVHKAMDIEYNDMFNNFTVSFWLRVPKVSASHLEQYGTNEYSIISSMKKHSLSIGSGWSVSLKGNNLIWTLKDSAGEVRQITFRDLPDKFNAYLANKWVFITITNDRLSSANLYINGVLMGSAEITGLGAIREDNNITLKLDRCNNNNQYVSIDKFRIFCKALNPKEIEKLYTSYLSITFLRDFWGNPLRYDTEYYLIPVASSSKDVQLKNITDYMYLTNAPSYTNGKLNIYYRRLYNGLKFIIKRYTPNNEIDSFVKSGDFIKLYVSYNNNEHIVGYPKDGNAFNNLDRILRVGYNAPGIPLYKKMEAVKLRDLKTYSVQLKLYDDKNASLGLVGTHNGQIGNDPNRDILIASNWYFNHLKDKILGCDWYFVPTDEGWTND hspa1a_hspa1b_human-1 HSPA1A_HSPA1B_human molecule set molecule set human Homo sapiens Human P0DMV8,P0DMV9 drb1_human HLA class II histocompatibility antigen, DRB1 beta chain HLA-DRB1 Human leukocyte antigen DRB1 protein protein human Homo sapiens Human MVCLKLPGGSCMTALTVTLMVLSSPLALSGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS hla_drb1-dnak-1 2964323 N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 2964323 1A, 3 HLA-DRB1*0801 allele The DRB1*0402 peptide did not inhibit binding, but all other peptides tested did. low pH hla_drb1-dnak-2 2964323 N/A 2964326 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 1B-1 HLA-DRB1*0801 allele Inhibitor:HLA-DRB1*0901/DRB1*090102 allele (0901 sequence shown to contain errors and be identical to DRB1*09:01:02 (December 1996)). hla_drb1-dnak-3 2964323 n/a 2964327 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false p.[Lys100Arg;Ala103Glu] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 1B-2 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0403 hla_drb1-dnak-4 2964323 n/a 2964327 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 1B-3 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0401 hla_drb1-dnak-5 2964323 N/A 2964328 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 1B-4 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0101 hla_drb1-dnak-6 2964323 N/A 2964325 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 1B-5 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0801 dnak-hla_drb1-1 2964323 N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 2A The DRB1*0402 peptide did not inhibit binding, but all other peptides tested did. dnak-hla_drb1-2 2964323 N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964325 inhibitor inhibitor prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-7 Inhibitor:HLA-DRB1*0801 allele dnak-hla_drb1-3 2964323 n/a 2964327 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-1 Inhibitor: HLA-DRB1*0401 dnak-hla_drb1-4 2964323 N/A 2964328 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-4 Inhibitor: HLA-DRB1*0101 dnak-hla_drb1-5 2964323 N/A 2964326 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-5 Inhibitor:HLA-DRB1*0901/DRB1*090102 allele (0901 sequence shown to contain errors and be identical to DRB1*09:01:02 (December 1996)). dnak-hla_drb1-6 2964323 n/a 2964327 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false p.[Lys100Arg;Ala103Glu] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-6 Inhibitor: HLA-DRB1*0403 dnak-cd74-1 2964323 N/A 2964329 inhibitor inhibitor prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-8 dnak-cd74-2 2964323 N/A 2964330 inhibitor inhibitor prey prey fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2A-9 hspa1a-cd74-1 2964323 N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2B, 7A Unlabeled HLA-DR-derived peptide fragments showed very weak competition ATP hspa1a-cd74-2 2964323 N/A 2964329 inhibitor inhibitor prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 2Bi Unlabeled CLIP peptide as well as a peptide from tetanus toxin (TT 947–966) showed stronger competition as compared to the HLA-DR-derived peptide fragments. hspa1a-cd74-3 2964323 N/A 2964330 inhibitor inhibitor prey prey fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964329 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 2Bii Unlabeled CLIP peptide as well as a peptide from tetanus toxin (TT 947–966) showed stronger competition as compared to the HLA-DR-derived peptide fragments. dnak-hla_drb1-7 2964323 N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4 A/B, 5, 6,7B HLA-DRB1*0401 Binding is greater at pH 5 than pH 7. Hsc70 also bound HLA-DRB1*0401 to a greater extent at pH=5 than pH 7, but the origins of this molecule could not be clarified. pH7 - Kd =113nM (as shown in figure 5) pH5 - Kd = 15nM (as shown in figure 5) From several experiments, a KD of 130 nM was calculated at pH 7 and of 26.6 nM at pH 5. (results not shown) ATP dnak-hla_drb1-8 2964323 N/A 2964332 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964324 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4 A/Bi HLA-DRB1*0401 weak binding hspa1a_hspa1b_human-hla_drb1-1 2964323 N/A 2964332 inhibitor inhibitor prey prey human-ihw 9041/jo 528239 human-ihw 9041/jo 528239 B Lymphoblastoid Cell Line Human ihw 9041/jo 528239 IHW 9041/JO 528239 http://www.ebi.ac.uk/cgi-bin/ipd/imgt/hla/fetch_cell.cgi?11636 N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 6-1 HLA-DRB1*0401;Inhibitor HLA-DRB1*1104 hspa1a_hspa1b_human-hla_drb1-2 2964323 n/a 2964332 inhibitor inhibitor prey prey human-bm9 human-BM9_B Lymphoblastoid Cell Line Human bm9 bm9-IHW 9068 EBV transformed B lymphoblastoid cell line http://www.ebi.ac.uk/cgi-bin/ipd/imgt/hla/fetch_cell.cgi?11619 N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 6-2 HLA-DRB1*0401;Inhibitor HLA-DRB1*0801 hspa1a_hspa1b_human-hla_drb1-3 2964323 n/a 2964332 inhibitor inhibitor prey prey human-pitout human-Pitout IHW9051 Human pitout PITOUT-IHW 9051 B Lymphoblastoid Cell Line http://web.expasy.org/cellosaurus/CVCL_E809 N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 6-3 HLA-DRB1*0401;Inhibitor HLA-DRB1*0701 hspa1a_hspa1b_human-hla_drb1-4 2964323 N/A 2964332 inhibitor inhibitor prey prey human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 6-4 HLA-DRB1*0401;Inhibitor HLA-DRB1*0401 Using recombinant HLA-DRB1*0401 lacking the transmembrane domain produced the same result. hspa1a_hspa1b_human-hla_drb1-5 2964323 n/a 2964327 inhibitor inhibitor prey prey p.[Leu96Ile;Gln99Asp;Lys100Glu;Gly115Val] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-al10 AL10- EBV-transformed B lymhoblastoid cell line from RA patient Human al10 AL10- EBV-transformed B lymhoblastoid cell line from RA patient N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 6-5 HLA-DRB1*0401;Inhibitor HLA-DRB1*0402 Using recombinant HLA-DRB1*0402 lacking the transmembrane domain produced the same result. hspa1a_hspa1b_human-hla_drb1-6 2964323 n/a 2964332 inhibitor inhibitor prey prey P01911:p.Val115Gly mutation mutation certain certain sequence position certain certain certain sequence position certain false human-hor human HOR/IHW 9053 B Lymphoblastoid Cell Line Human hor-ihw 9053 HOR-IHW 9053 http://www.ebi.ac.uk/cgi-bin/ipd/imgt/hla/fetch_cell.cgi?10757 N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 6-6 HLA-DRB1*0401;Inhibitor HLA-DRB1*1302 hspa1a_hspa1b_human-hla_drb1-7 2964323 n/a 2964327 inhibitor inhibitor prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2964332 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 N/A 2964331 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false 7C HLA-DRB1*0401;Inhibitor HLA-DRB1*0401 The interaction of Hsp70 with complete HLA-DR molecules cannot be significantly inhibited by HV3-region peptide fragments.