DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ westlake-2007-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. DIP original experiment: DIP-260599X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene. Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. Only protein-protein interactions imex curation westlake-2007-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. DIP original experiment: DIP-260604X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene. Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. Only protein-protein interactions imex curation westlake-2007-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. DIP original experiment: DIP-260602X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene. Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. Only protein-protein interactions imex curation westlake-2007-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. DIP original experiment: DIP-260600X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene. Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. Only protein-protein interactions imex curation westlake-2007-5 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. DIP original experiment: DIP-260603X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene. Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. Only protein-protein interactions imex curation westlake-2007-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. DIP original experiment: DIP-260601X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene. Westlake CJ., Junutula JR., Simon GC., Pilli M., Prekeris R., Scheller RH., Jackson PK., Eldridge AG. Only protein-protein interactions imex curation rb11a_human Ras-related protein Rab-11A YL8 RAB11A RAB11 protein protein human Homo sapiens Human MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI evi5_human Ecotropic viral integration site 5 protein homolog Neuroblastoma stage 4S gene protein EVI5 NB4S protein protein human Homo sapiens Human MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPALSPSSPSQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLSHLEEDSWILWGRIVNEWEDVRKKKEKQVKELVHKGIPHHFRAIVWQLLCSAQSMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHNFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEHLPELFVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRIFDIFMSEGLEIVFRVGLALLQMNQAELMQLDMEGMLQHFQKVIPHQFDGVPDKLIQAAYQVKYNSKKMKKLEKEYTTIKTKEMEEQVEIKRLRTENRLLKQRIETLEKHKCSSNYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLDIEKRNNSLPDENNIARLQEELIAVKLREAEAIMGLKELRQQVKDLEEHWQRHLARTTGRWKDPPKKNAMNELQDELMTIRLREAETQAEIREIKQRMMEMETQNQINSNHLRRAEQEVISLQEKVQYLSAQNKGLLTQLSEAKRKQAEIECKNKEEVMAVRLREADSIAAVAELRQHIAELEIQKEEGKLQGQLNKSDSNQYIGELKDQIAELNHELRCLKGQRGFSGQPPFDGIHIVNHLIGDDESFHSSDEDFIDNSLQETGVGFPLHGKSGSMSLDPAVADGSESETEDSVLETRESNQVVQKERPPRRRESYSTTV rb11b_human Ras-related protein Rab-11B GTP-binding protein YPT3 RAB11B YPT3 protein protein human Homo sapiens Human MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCCQNL evi5-rab11a-1 3152178 N/A 3152184 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3152185 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rab11a-evi5-1 3152179 N/A 3152184 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3152185 unspecified role unspecified role prey prey MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false rab11a-evi5-2 3152180 N/A 3152184 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3152185 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false rab11a-evi5-3 3152181 N/A 3152184 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3152185 unspecified role unspecified role prey prey MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false evi5-rab11b-1 3152182 N/A 3152186 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3152185 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false evi5-rab11a-2 3152183 N/A 3152184 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3152185 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false