DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ rudolph-2007-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. DIP original experiment: DIP-66403X Structure (London, England : 1993) 2007 IMEx Structure of the Bateman2 domain of yeast Snf4: dimeric association and relevance for AMP binding. Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. Only protein-protein interactions imex curation rudolph-2007-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. DIP original experiment: DIP-66400X Structure (London, England : 1993) 2007 IMEx Structure of the Bateman2 domain of yeast Snf4: dimeric association and relevance for AMP binding. Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. Only protein-protein interactions imex curation rudolph-2007-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. DIP original experiment: DIP-66401X Structure (London, England : 1993) 2007 IMEx Structure of the Bateman2 domain of yeast Snf4: dimeric association and relevance for AMP binding. Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. Only protein-protein interactions imex curation rudolph-2007-4 ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. DIP original experiment: DIP-66402X Structure (London, England : 1993) 2007 IMEx Structure of the Bateman2 domain of yeast Snf4: dimeric association and relevance for AMP binding. Rudolph MJ., Amodeo GA., Iram SH., Hong SP., Pirino G., Carlson M., Tong L. Only protein-protein interactions imex curation aakg_yeast 5'-AMP-activated protein kinase subunit gamma SNF4 CAT3 Regulatory protein CAT3 SCI1 Sucrose non-fermenting protein 4 YGL115W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKPTQDSQEKVSIEQQLAVESIRKFLNSKTSYDVLPVSYRLIVLDTSLLVKKSLNVLLQNSIVSAPLWDSKTSRFAGLLTTTDFINVIQYYFSNPDKFELVDKLQLDGLKDIERALGVDQLDTASIHPSRPLFEACLKMLESRSGRIPLIDQDEETHREIVVSVLTQYRILKFVALNCRETHFLKIPIGDLNIITQDNMKSCQMTTPVIDVIQMLTQGRVSSVPIIDENGYLINVYEAYDVLGLIKGGIYNDLSLSVGEALMRRSDDFEGVYTCTKNDKLSTIMDNIRKARVHRFFVVDDVGRLVGVLTLSDILKYILLGSN 51B387E346EE9561 snf4-snf4-1 3152040 N/A 3152044 unspecified role unspecified role prey prey LexA-tag LexA-tag lexa dna bd lexa dna binding domain lexa dna bd n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3152044 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false snf4-1 3152041 N/A 3152044 unspecified role unspecified role unspecified role unspecified role Region 179-322 Region 179-322 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false snf4-2 3152042 N/A 3152044 unspecified role unspecified role unspecified role unspecified role Region 179-322 Region 179-322 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false snf4-3 3152043 N/A 3152044 unspecified role unspecified role unspecified role unspecified role Region 179-322 Region 179-322 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false