DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mclellan-2006-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-264125X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a heparin-dependent complex of Hedgehog and Ihog. McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2006-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-264130X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a heparin-dependent complex of Hedgehog and Ihog. McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2006-3 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-264128X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a heparin-dependent complex of Hedgehog and Ihog. McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2006-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-264126X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a heparin-dependent complex of Hedgehog and Ihog. McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2006-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-264129X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a heparin-dependent complex of Hedgehog and Ihog. McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2006-6 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-264127X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structure of a heparin-dependent complex of Hedgehog and Ihog. McLellan JS., Yao S., Zheng X., Geisbrecht BV., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation ihog_drome Interference hedgehog ihog CG9211 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTLLTSSLLLFSLLTSRLEAIPVLEKSPAHPAHSAHPAHPAHPAHPAHPSPGVRILRAPESLVAPLGDEVVLECETSLQPERFEWSHRSSRSPGAGFKYLKTGTAKANVSQEAAISRLRVLVRPDTLGEYRCVGWFGPLVVTSTIARLELASTSLVDAQESESPLQWRVSAGNSVLWSCGQQVQSNPSASWSYYRNGVEIKPEFIGTNGNLFLSNVSSESSGSYSCQATNPASGERIQLPGSLQLQVTPEQRSESKSPHLLRGQPSSQEITIREGSSLLLLCPGVGSPPPTVVWSSPDVVGAVKNKRSKVFGHALEISNTRVNDAGTYICFQDNGVRPALEHYIKVHVEQPPQIVRPPWADLTNEGDRLKLECKATGVPTPEIYWLLNGHSSIDDSEAELSNNFLILHSVLKRHAGYVQCFARNRLGEHSAGTLLQVNPKQIQEPRESGGTHRPKPNQGSRQKQMYPPTPPNVTRLSDESVMLRWMVPRNDGLPIVIFKVQYRMVGKRKNWQTTNDNIPYGKPKWNSELGKSFTASVTDLKPQHTYRFRILAVYSNNDNKESNTSAKFYLQPGAALDPMPVPELLEIEEYSETAVVLHWSLASDADEHLITGYYAYYRPSSSAGEYFKATIEGAHARSFKIAPLETATMYEFKLQSFSAASASEFSALKQGRTQRPKTSTTEEPTLQMGDRDTTTPSHNETFNMSPMLTGTIGGGAVLILLLISTCFCVCRRRNSRSRGNNPNKPRMAELRDDFVPLGNCSPTKQRQRTRHIHITLNPLAQQQQQALEEKNDTDQDAPYYQRPSSYDYDPTLRRMSSSSLRRSQRTLERAGGSNGSNNGNNNNLNQSAEAGSIENPGKPGRVLMKRPRLSSRSENLSSGSLNSVGV q02936-1 Protein hedgehog hh CG4637 A B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDNHSSVPWASAASVTCLSLDAKCHSSSSSSSSKSAASSISAIPQEETQTMRHIAHTQRCLSRLTSLVALLLIVLPMVFSPAHSCGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLESGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHTDGGAVLTVTPAHLVSVWQPESQKLTFVFADRIEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLEAWLPAKEQLHSSPKVVSSAQQQNGIHWYANALYKVKDYVLPQSWRHD hh-ihog-1 3149917 N/A 3149923 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3149924 unspecified role unspecified role unspecified role unspecified role Region 85-248 Region 85-248 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ihog-1 3149918 N/A 3149923 unspecified role unspecified role unspecified role unspecified role Region 466-577 Region 466-577 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hh-ihog-2 3149919 N/A 3149923 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3149924 unspecified role unspecified role unspecified role unspecified role Region 85-248 Region 85-248 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ihog-2 3149920 N/A 3149923 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hh-ihog-3 3149921 N/A 3149923 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3149924 unspecified role unspecified role unspecified role unspecified role Region 85-248 Region 85-248 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ihog-3 3149922 N/A 3149923 unspecified role unspecified role unspecified role unspecified role Region 466-577 Region 466-577 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false