DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ alam-2006-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. DIP original experiment: DIP-66577X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. Only protein-protein interactions imex curation alam-2006-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. DIP original experiment: DIP-66579X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. Only protein-protein interactions imex curation alam-2006-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. DIP original experiment: DIP-66578X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG vps36_human Vacuolar protein-sorting-associated protein 36 VPS36 C13orf9 EAP45 ELL-associated protein of 45 kDa ESCRT-II complex subunit VPS36 CGI-145 protein protein human Homo sapiens Human MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS ub-vps36-1 3149603 N/A 3149606 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3149607 unspecified role unspecified role neutral component neutral component 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-vps36-2 3149604 N/A 3149606 unspecified role unspecified role bait bait GST Tag GST Tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false in vitro In vitro N/A 3149607 unspecified role unspecified role prey prey 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false vps36-ub-1 3149605 N/A 3149606 unspecified role unspecified role prey prey ile44ala I44A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3149607 unspecified role unspecified role bait bait 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q86VN1:p.Val67Ala V67A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q86VN1:p.Phe68Ala F68A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q86VN1:p.Glu70Ala E70A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false