DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hirano-2006-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66573X Nature structural & molecular biology 2006 IMEx Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. Only protein-protein interactions imex curation hirano-2006-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66574X Nature structural & molecular biology 2006 IMEx Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. Only protein-protein interactions imex curation vps36_mouse Vacuolar protein-sorting-associated protein 36 Vps36 ESCRT-II complex subunit VPS36 protein protein mouse Mus musculus Mouse MDRFVWTSGLLEINETLVIQQRGVRVYDGEEKIKFDAGTLLLSTHRLIWRDQKNNECCMAIPLSQIVFIEEQAAGIGKSAKIVVHLHPAPSNKEPGPFQSSKNSYIRLSFKEHGQIEFYRRLSEEMTQRRWETVPVSQSLQTNKGPQPGRVRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKEKQGDVTEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQAPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPIRLRVFDSGVMVIELQTHKEEEMVASALETVSERGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQN mint Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub-vps36-3 3149575 N/A 3149577 unspecified role unspecified role neutral component neutral component 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3149578 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false vps36-ub-2 3149576 N/A 3149577 unspecified role unspecified role bait bait 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false Q91XD6:p.Val67Ala V67A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q91XD6:p.Phe68Ala F68A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q91XD6:p.Glu70Ala E70A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q91XD6:p.Val83Ala V83A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q91XD6:p.His85Ala H85A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3149578 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false