DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gwizdek-2006-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264030X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264034X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264032X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264035X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-5 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264037X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264033X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-7 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264031X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-8 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264036X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation gwizdek-2006-9 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. DIP original experiment: DIP-264038X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. Gwizdek C., Iglesias N., Rodriguez MS., Ossareh-Nazari B., Hobeika M., Divita G., Stutz F., Dargemont C. Only protein-protein interactions imex curation mex67_yeast mRNA export factor MEX67 MEX67 P2520 YPL169C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGFHNVGNINMMAQQQMQQNRIKISVRNWQNATMNDLINFISRNARVAVYDAHVEGPLVIGYVNSKAEAESLMKWNGVRFAGSNLKFELLDNNGASAGTSDTISFLRGVLLKRYDPQTKLLNLGALHSDPELIQKGVFSSISTQSKMFPAMMKLASTEKSLIVESVNLADNQLKDISAISTLAQTFPNLKNLCLANNQIFRFRSLEVWKNKFKDLRELLMTNNPITTDKLYRTEMLRLFPKLVVLDNVIVRDEQKLQTVYSLPMKIQQFFFENDALGQSSTDFATNFLNLWDNNREQLLNLYSPQSQFSVSVDSTIPPSTVTDSDQTPAFGYYMSSSRNISKVSSEKSIQQRLSIGQESINSIFKTLPKTKHHLQEQPNEYSMETISYPQINGFVITLHGFFEETGKPELESNKKTGKNNYQKNRRYNHGYNSTSNNKLSKKSFDRTWVIVPMNNSVIIASDLLTVRAYSTGAWKTASIAIAQPPQQQASVLPQVASMNPNITTPPQPQPSVVPGGMSIPGAPQGAMVMAPTLQLPPDVQSRLNPVQLELLNKLHLETKLNAEYTFMLAEQSNWNYEVAIKGFQSSMNGIPREAFVQF 6CE55EEC6FA40B40 hpr1_yeast THO complex subunit HPR1 HPR1 YDR138W YD9302.14 Hyperrecombination protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNTEELIQNSIGFLQKTFKALPVSFDSIRHEPLPSSMLHASVLNFEWEPLEKNISAIHDRDSLIDIILKRFIIDSMTNAIEDEEENNLEKGLLNSCIGLDFVYNSRFNRSNPASWGNTFFELFSTIIDLLNSPSTFLKFWPYAESRIEWFKMNTSVEPVSLGESNLISYKQPLYEKLRHWNDILAKLENNDILNTVKHYNMKYKLENFLSELLPINEESNFNRSASISALQESDNEWNRSARERESNRSSDVIFAADYNFVFYHLIICPIEFAFSDLEYKNDVDRSLSPLLDAILEIEENFYSKIKMNNRTRYSLEEALNTEYYANYDVMTPKLPVYMKHSNAMKMDRNEFWANLQNIKESDDYTLRPTIMDISLSNTTCLYKQLTQEDDDYYRKQFILQLCFTTNLIRNLISSDETRNFYKSCYLRENPLSDIDFENLDEVNKKRGLNLCSYICDNRVLKFYKIKDPDFYRVIRKLMSSDEKFTTAKIDGFKEFQNFRISKEKIPPPAFDETFKKFTFIKMGNKLINNVWKIPTGLDKIEQEVKKPEGVYEAAQAKWESKISSETSGGEAKDEIIRQWQTLRFLRSRYLFDFDKVNEKTGVDGLFEEPRKVEALDDSFKEKLLYKINQEHRKKLQDAREYKIGKERKKRALEEEASFPEREQKIKSQRINSASQTEGDELKSEQTQPKGEISEENTKIKSSEVSSQDPDSGVAGEFAPQNTTAQLENPKTEDNNAATSNISNGSSTQDMK CBD92432F099AD22 ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein thp2_yeast THO complex subunit THP2 THP2 YHR167W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSDDLLTESLKKLPDLHRELINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISEYYSTFEHLNRDTLRYINLLKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQYCAPDTDIRGVDAQIKNYLDQIKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIEMLMFGDDAHSMKKMIQKIDSLKSEINAPSESYPVDKEGDIVLE 16EFA6F464507C57 rad23_yeast UV excision repair protein RAD23 SYGP-ORF29 YEL037C RAD23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSLTFKNFKKEKVPLDLEPSNTILETKTKLAQSISCEESQIKLIYSGKVLQDSKTVSECGLKDGDQVVFMVSQKKSTKTKVTEPPIAPESATTPGRENSTEASPSTDASAAPAATAPEGSQPQEEQTATTERTESASTPGFVVGTERNETIERIMEMGYQREEVERALRAAFNNPDRAVEYLLMGIPENLRQPEPQQQTAAAAEQPSTAATTAEQPAEDDLFAQAAQGGNASSGALGTTGGATDAAQGGPPGSIGLTVEDLLSLRQVVSGNPEALAPLLENISARYPQLREHIMANPEVFVSMLLEAVGDNMQDVMEGADDMVEGEDIEVTGEAAAAGLGQGEGEGSFQVDYTPEDDQAISRLCELGFERDLVIQVYFACDKNEEAAANILFSDHAD B3F0436DAB60B833 mex67-hpr1-1 3149500 N/A 3149509 unspecified role unspecified role bait bait Region 543-599 Region 543-599 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3149510 unspecified role unspecified role prey prey Region 548-752 Region 548-752 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false mex67-hpr1-2 3149501 N/A 3149509 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3149510 unspecified role unspecified role prey prey Region 548-752 Region 548-752 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false mex67-hpr1-3 3149502 N/A 3149510 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3149509 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false mex67-ub-1 3149503 N/A 3149511 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3149509 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false mex67-ub-2 3149504 N/A 3149511 unspecified role unspecified role unspecified role unspecified role Dansyl-label Dansyl-label dansyl label dansyl label 5-dimethylaminonaphthalene-1-sulfonyl tag dansyl tag 5-dimethylaminonaphthalene-1-sulfonyl tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3149509 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 543-599 decreased the interaction rate/strength. mex67-thp2-1 3149505 N/A 3149512 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3149509 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hpr1-mex67-1 3149506 N/A 3149509 unspecified role unspecified role prey prey Region 543-599 Region 543-599 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3149510 unspecified role unspecified role bait bait Region 548-752 Region 548-752 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false ub-hpr1-1 3149507 N/A 3149510 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3149511 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rad23-ub-4 3149508 N/A 3149513 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3149511 unspecified role unspecified role unspecified role unspecified role Dansyl-label Dansyl-label dansyl label dansyl label 5-dimethylaminonaphthalene-1-sulfonyl tag dansyl tag 5-dimethylaminonaphthalene-1-sulfonyl tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli direct interaction direct interaction false false false