DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shen-2006-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254914X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254924X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254915X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-6 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254919X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254925X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-8 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254920X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-9 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254916X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-10 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254921X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-11 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254917X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-12 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254926X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-13 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254922X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-14 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254923X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation shen-2006-15 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shen Y., Joachimiak A., Rosner MR., Tang WJ. DIP original experiment: DIP-254918X Nature 2006 IMEx Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Shen Y., Joachimiak A., Rosner MR., Tang WJ. Only protein-protein interactions imex curation p14735-1 Insulin-degrading enzyme IDE Abeta-degrading protease Insulin protease Insulysin protein protein human Homo sapiens Human MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL p05067-pro_0000000093 Amyloid-beta protein 40 APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 672 711 ins_human Insulin INS protein protein human Homo sapiens Human MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver mint homomint Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730] iapp_human Islet amyloid polypeptide IAPP Amylin Diabetes-associated peptide Insulinoma amyloid peptide protein protein human Homo sapiens Human MGILKLQVFLIVLSVALNHLKATPIESHQVEKRKCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTYGKRNAVEVLKREPLNYLPL not sure about the protein origin mint gluc_human Pro-glucagon GCG protein protein human Homo sapiens Human MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK mint homomint ide-ide-1 3149394 N/A 3149407 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3149407 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false app-ide-1 3149395 N/A 3149408 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3149407 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ide-ins-1 3149396 N/A 3149409 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 unspecified role unspecified role unspecified role unspecified role Region 42-1019 Region 42-1019 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ide-ins-2 3149397 N/A 3149409 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false iapp-ide-1 3149398 N/A 3149410 unspecified role unspecified role unspecified role unspecified role Region 34-70 Region 34-70 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false app-ide-2 3149399 N/A 3149408 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3149407 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false app-ide-3 3149400 N/A 3149408 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3149407 unspecified role unspecified role unspecified role unspecified role Region 42-1019 Region 42-1019 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false iapp-ide-2 3149401 N/A 3149410 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 34-70 Region 34-70 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false iapp-ide-3 3149402 N/A 3149410 unspecified role unspecified role unspecified role unspecified role Region 34-70 Region 34-70 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 unspecified role unspecified role unspecified role unspecified role Region 42-1019 Region 42-1019 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gcg-ide-1 3149403 N/A 3149411 unspecified role unspecified role unspecified role unspecified role Region 53-81 Region 53-81 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gcg-ide-2 3149404 N/A 3149411 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 53-81 Region 53-81 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false ide-ins-3 3149405 N/A 3149409 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gcg-ide-3 3149406 N/A 3149411 unspecified role unspecified role unspecified role unspecified role Region 53-81 Region 53-81 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3149407 unspecified role unspecified role unspecified role unspecified role Region 42-1019 Region 42-1019 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false