DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chigri-2006-1 pea Pisum sativum (Garden pea) Garden pea pull down pull down ms participant Identification by mass spectrometry ms participant Chigri F., Hörmann F., Stamp A., Stammers DK., Bölter B., Soll J., Vothknecht UC. DIP original experiment: DIP-263992X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Calcium regulation of chloroplast protein translocation is mediated by calmodulin binding to Tic32. Chigri F., Hörmann F., Stamp A., Stammers DK., Bölter B., Soll J., Vothknecht UC. Only protein-protein interactions imex curation chigri-2006-2 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Chigri F., Hörmann F., Stamp A., Stammers DK., Bölter B., Soll J., Vothknecht UC. DIP original experiment: DIP-263993X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Calcium regulation of chloroplast protein translocation is mediated by calmodulin binding to Tic32. Chigri F., Hörmann F., Stamp A., Stammers DK., Bölter B., Soll J., Vothknecht UC. Only protein-protein interactions imex curation chigri-2006-3 pea Pisum sativum (Garden pea) Garden pea anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chigri F., Hörmann F., Stamp A., Stammers DK., Bölter B., Soll J., Vothknecht UC. DIP original experiment: DIP-263994X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Calcium regulation of chloroplast protein translocation is mediated by calmodulin binding to Tic32. Chigri F., Hörmann F., Stamp A., Stammers DK., Bölter B., Soll J., Vothknecht UC. Only protein-protein interactions imex curation tic32_pea Short-chain dehydrogenase TIC 32, chloroplastic Translocon at the inner envelope membrane of chloroplasts 32 TIC32 HP32 IEP32 protein protein pea Pisum sativum (Garden pea) Garden pea MWPFSSKKGVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNSAVNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLWDFSINLVKQK q6lcy3_pea protein protein pea Pisum sativum (Garden pea) Garden pea MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVMMAK calm_bovin Calmodulin CALM CAM protein protein bovin Bos taurus (Bovine) Bovine MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK ti110_pea Protein TIC110, chloroplastic TIC110 IEP110 Chloroplast inner envelope protein, 110 kDa IAP100 Translocon at the inner envelope membrane of chloroplasts 110 protein protein pea Pisum sativum (Garden pea) Garden pea MNPSTLKPSHTHPSLLLPAPSPLRTQRRRFRVSLPRCSSDTNNPASSSSPPQRPPKELNGIEILVDKLSSPARLATSAVIVAGAVAAGYGLGSRFGGSRNAALGGAVALGAAGGAAAYALNAAAPQVAAVNLHNYVAGFDDPSILTREDIEVIANKYGVSKQDEAFKAEICDIYSEFVSSVIPPGGEELKGDEVDKIVNFKSSLGLDDPDAAAVHMEIGRKLFRQRLEVGDREGGVEQRRAFQKLIYVSNIVFGDASSFLLPWKRVFKVTESQVEVAIRDNAQRLYASKLKSVGRDFDLGKLVTLKETQSLCCLSDELAENLFREHARKLVEENISVALGILKSRTRAVPGVSQVVEEIEKVLAFNDLLISFKNHSDIDRLARGVGPVSLVGGEYDADRKIEDLKLLYRAYVSDALSSGRMEDNKFAALNQLKNIFGLGKREAEAILLDITRKVYRKRLGQTVSSGELEMADSKAAFLQNLCDELHFDPQKASELHEEIYRQKLQQCVADGELTDENVAALLKLRVMLCVPQQTVEAAHAEICGNLFEKIVKDAIASGVDGYDDETKKSVRKAAHGLRLTKETALSIASKAVRKMFITYVKRSRSAKGNGESAKELKKLIAFNTLVVTKLVEDIKGESPDVKIEEPKIEEPEEIRESEEYEMRITSDTQENKTGQRACRKDGKAWSDRITLKDDLPEKDRADLYKTFLTYCLTGDVVRIPFGVEIKKKKDDTEYIYLNQLGGILGLTGKVIMDVHRGLAEQAFRKQAEVLLADGQLTKARVEQLGKMQKEIGLSQEYAQKIIKNITTTKMAAAIETAVTQGKLNMKQIRELKESNVDLDSMVSVSLRETIFKKTVGDIFSSGTGEFDEEEVYEKIPLDLNINKEKARGVVCELAQNRLSNSLIQAVALLRQRNHKGVVFSLNNLLACDKAVPSQTLSWEVSEELSDLYTIYLKSDPSPEKLSRLQYLLGINDSTAAALRDSEDSLLETAEEEKFVF This sequence has been withdrawn from Uniprot. E36649FF7A0C2680 tic62_pea Protein TIC 62, chloroplastic Translocon at the inner envelope membrane of chloroplasts 62 TIC62 protein protein pea Pisum sativum (Garden pea) Garden pea MPMEVFSLTSTAIPSTLTRRDTAADKPSPHLNLSKYSHFMRYPLTTTLTNNRIRSSSSSSSSIRAQASGSTKSSTAEGIPEKTDSKDDNLVFVAGATGKVGSRTVRELIKLGFKVRAGVRNAQKAGALVQSVKQLKLDGASGGGEAVEKLEIVECDLEKADQIGSALGNASTVICAIGASEKEIFDITGPCRIDYRATKNLVDAATVAKVNHFILVTSLGTNKFGLPAAILNLFWGVLIWKRKAEEALLASGIPYTIVRPGGMERPTDAYKETHNVTLSTEDTLFGGQVSNLQVAELMAIMAKNPDLSYCKIVEVIAETTAPLTPAEKLLTRIPSQRPYIPSPKKVQKADTATVSNTGPSANVVAEVPSIAPQKETASKPVAKTEQPLSPYTAYDDLKPPSSPSPTKPSEKKQINISDAVPTPISSDTPSSIQEIDGISQTTSSSKGKESLSPYAAYPDLKPPSSPSPSVPTTSLSKRDTVVVSSNGPAQLSVEDTPKNEEQHLHEPKSRPLSPYAMYEDLKPPASPSPSFRKS q6lcy3_pea-tic32-1 3149175 N/A 3149178 unspecified role unspecified role prey prey pea Pisum sativum (Garden pea) Garden pea N/A 3149179 unspecified role unspecified role bait bait pea Pisum sativum (Garden pea) Garden pea physical association physical association false false false tic32-calm-1 3149176 N/A 3149180 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine N/A 3149178 unspecified role unspecified role bait bait Region 155-316 Region 155-316 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tic110-tic32-1 3149177 N/A 3149181 unspecified role unspecified role bait bait pea Pisum sativum (Garden pea) Garden pea N/A 3149182 unspecified role unspecified role prey prey pea Pisum sativum (Garden pea) Garden pea N/A 3149178 unspecified role unspecified role prey prey pea Pisum sativum (Garden pea) Garden pea association association false false false