DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ van-2006-9 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Van Wagoner RM., Clardy J. DIP original experiment: DIP-66364X Structure (London, England : 1993) 2006 IMEx FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. Van Wagoner RM., Clardy J. Only protein-protein interactions imex curation van-2006-10 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Van Wagoner RM., Clardy J. DIP original experiment: DIP-66365X Structure (London, England : 1993) 2006 IMEx FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. Van Wagoner RM., Clardy J. Only protein-protein interactions imex curation van-2006-11 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Van Wagoner RM., Clardy J. DIP original experiment: DIP-66366X Structure (London, England : 1993) 2006 IMEx FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. Van Wagoner RM., Clardy J. Only protein-protein interactions imex curation q8knz7_9bact protein protein uncultured bacterium cslc2 uncultured bacterium cslc2 MTPRKVARILVAPNERDAARRIVRTTYEAQGYAIDESFATFLEGPSATTFGLFNGEVLYGTISIINDGAQGLPMDSIYAVELAAWRGEGKKLAEVVQFAMDHTLYEAVAGAKPSPFEAASLFTMVLTYALETHIDYLCISINPKHDTFYSLLGFTQIGALKHYGTVNAPAIARALYVPEWRSQTLLAQFMDAERSH 9707700FFB3EEB65 q8knz8_9bact protein protein uncultured bacterium cslc2 uncultured bacterium cslc2 MTYHDMDSRDTIIEIIADTLQVPREQVREESKLIDLAKDSIALFELLIRLEKQFSHKVKYEDIAHIETVGDISAFIGALPSATVLPLSQESVA B33F762EDB9D9E26 feem-1 3147682 N/A 3147685 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false feem-2 3147683 N/A 3147685 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false feel-feem-1 3147684 N/A 3147686 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro N/A 3147685 enzyme enzyme unspecified role unspecified role Q8KNZ7:p.Phe148Asp F148D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8KNZ7:p.His145Glu H145E, N, R mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false Q8KNZ7:p.His145Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false Q8KNZ7:p.His145Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false