DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ leng-2006-1 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263451X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263446X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-3 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263449X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263453X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263447X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-6 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263452X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-7 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263450X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation leng-2006-8 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. DIP original experiment: DIP-263448X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Leng M., Chan DW., Luo H., Zhu C., Qin J., Wang Y. Only protein-protein interactions imex curation blm_human Bloom syndrome protein RecQ protein-like 3 DNA helicase, RecQ-like type 2 BLM RECQ2 RECQL3 protein protein human Homo sapiens Human MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS rs27_human 40S ribosomal protein S27 RPS27 MPS1 Metallopan-stimulin 1 Small ribosomal subunit protein eS27 protein protein human Homo sapiens Human MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH plk1-blm-1 3146236 N/A 3146244 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3146245 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false blm-rps27-1 3146237 N/A 3146244 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3146246 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false blm-rps27-2 3146238 N/A 3146244 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-159 Region 1-159 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3146246 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false blm-plk1-1 3146239 N/A 3146244 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3146245 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false blm-rps27-3 3146240 N/A 3146244 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3146246 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false plk1-blm-2 3146241 N/A 3146244 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3146245 unspecified role unspecified role bait bait Region 326-603 Region 326-603 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P53350:p.[His538Ala;Lys540Met] H538A/K540M mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false plk1-blm-3 3146242 N/A 3146244 unspecified role unspecified role prey prey P54132:p.[Ser144Ala;Ser304Ala;Ser1290Ala;Ser1296Ala] S144A/S304A/S1290A/S1296A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3146245 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rps27-blm-1 3146243 N/A 3146244 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3146246 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false