DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ anselmo-2006-1 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263358X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263356X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-3 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263361X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263359X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263363X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263357X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-7 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263362X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation anselmo-2006-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. DIP original experiment: DIP-263360X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells. Anselmo AN., Earnest S., Chen W., Juang YC., Kim SC., Zhao Y., Cobb MH. Only protein-protein interactions imex curation wnk1_human Serine/threonine-protein kinase WNK1 WNK1 KDP KIAA0344 PRKWNK1 Protein kinase with no lysine 1 Protein kinase lysine-deficient 1 Erythrocyte 65 kDa protein Kinase deficient protein HSN2 protein protein human Homo sapiens Human MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT oxsr1_human Serine/threonine-protein kinase OSR1 Oxidative stress-responsive 1 protein OXSR1 OSR1 KIAA1101 protein protein human Homo sapiens Human MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS oxsr1-wnk1-2 3145649 N/A 3145657 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3145658 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false oxsr1-wnk1-3 3145650 N/A 3145657 unspecified role unspecified role prey prey Region 1834-2020 Region 1834-2020 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3145658 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false oxsr1-wnk1-4 3145651 N/A 3145657 enzyme enzyme unspecified role unspecified role Region 193-483 Region 193-483 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3145658 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-344 Region 1-344 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false wnk1-oxsr1-1 3145652 N/A 3145657 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3145658 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false oxsr1-oxsr1-1 3145653 N/A 3145658 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3145658 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-344 Region 1-344 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false wnk1-oxsr1-2 3145654 N/A 3145657 unspecified role unspecified role bait bait Region 1834-1938 Region 1834-1938 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 1939-2382 Region 1939-2382 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3145658 unspecified role unspecified role prey prey Region 292-527 Region 292-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false oxsr1-1 3145655 N/A 3145658 putative self putative self unspecified role unspecified role Region 1-344 Region 1-344 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false oxsr1-oxsr1-2 3145656 N/A 3145658 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3145658 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-344 Region 1-344 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false