DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hershkovits-2006-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Hershkovits G., Bangio H., Cohen R., Katcoff DJ. DIP original experiment: DIP-263229X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Recruitment of mRNA cleavage/polyadenylation machinery by the yeast chromatin protein Sin1p/Spt2p. Hershkovits G., Bangio H., Cohen R., Katcoff DJ. Only protein-protein interactions imex curation hershkovits-2006-2 in vitro In vitro pull down pull down autoradiography autoradiography Hershkovits G., Bangio H., Cohen R., Katcoff DJ. DIP original experiment: DIP-263230X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Recruitment of mRNA cleavage/polyadenylation machinery by the yeast chromatin protein Sin1p/Spt2p. Hershkovits G., Bangio H., Cohen R., Katcoff DJ. Only protein-protein interactions imex curation fir1_yeast Factor interacting with REF2 FIR1 PIP1 Polymerase-interacting protein 1 PAP1-interacting protein YER032W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLPVTPVKSKVCSTLSIQHEMDHDQCRDVLCPRENLLAINRTNNIESVAIPRQRSSKNKKPHEHTQSQVRFSIPDPNEISQDSPLKIVFPKSGNETERRMSTSSLLMNSHGHLVDMHSKILVDVPKEVWKFHHNRRKKCESRHRKTRSDVRSNTSSSGKEPNHSRSKSLQSIIVDTMNTYRATEVETSINENTSNISQVSPLNLSFDRPPPLTPEKNLYLTPESPLNRYHLPVPLEISLPPYLSPQNKDKKRSSLVYDGDGYSQFQEGNTSSSTESSLEQPSSSYSDEDDSIPYAHHDVSFELNNADADKLLGIDENANVNLKIQRRNLKNPQHIKSKTDRECEEKNTEKNVSLKILSTPNKLIDIPDLEHMKSPPSTGLNGTLKFFQQFEPSEEPTSPTRQVNPESLDKLDMSFKFPSSTTNNNVDKVHENRNSGDTNNEDFLKVDTSPVNQSFESRRQMLIDLQKSPTNNNPRIHKHRRSRSVHNIDDISLNFEATSTPPAPTSAPSIPVEHSNPCTSIEIPKRSPLRFTSSPKTSDIPPEAQSPKNGSFLQEISVPSIQIIPDESISHTREPSPSLIECPEDENEAFSTEVADHSIAIISETKSVPSIEPFKPLSSFNSFGQEIQNKEPTPLNQTPTDLIGKQRNCVNPHSIPFSVLSSNSQSSQSGSSKSSYNSEFSSNTAITDTTSQPSVTINRSTLEHNFIEEKRSIKNLGHGPPSQKNNYSFPRNKNTPSNRHDLDFNTIYEKRDGKMVEVILLDEDEDVGLKNNDISRTRVCHAQKAKNEQQKKRLSHCNEILGMCDKTADDAKRIIYQLVNEKHKFSEKGQQTKPKKSRVLPPLPFPLYDEKGNSLIPNKYQSSIHNDIPSHRKLK DFD2AB3684EBC1D2 sro7_yeast Lethal(2) giant larvae protein homolog SRO7 SRO7 SNI1 YPR032W YP9367.12 SOP1 Suppressor of RHO3 protein 7 Polarity protein SRO7 Sodium protection protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGSKRLKNVKEAFKSLKGQNSETPIENSKASFKSKNSKTSTISKDAKSSSSLKIPISSNNKNKIFSLAETNKYGMSSKPIAAAFDFTQNLLAIATVTGEVHIYGQQQVEVVIKLEDRSAIKEMRFVKGIYLVVINAKDTVYVLSLYSQKVLTTVFVPGKITSIDTDASLDWMLIGLQNGSMIVYDIDRDQLSSFKLDNLQKSSFFPAARLSPIVSIQWNPRDIGTVLISYEYVTLTYSLVENEIKQSFIYELPPFAPGGDFSEKTNEKRTPKVIQSLYHPNSLHIITIHEDNSLVFWDANSGHMIMARTVFETEINVPQPDYIRDSSTNAAKISKVYWMCENNPEYTSLLISHKSISRGDNQSLTMIDLGYTPRYSITSYEGMKNYYANPKQMKIFPLPTNVPIVNILPIPRQSPYFAGCHNPGLILLILGNGEIETMLYPSGIFTDKASLFPQNLSWLRPLATTSMAASVPNKLWLGALSAAQNKDYLLKGGVRTKRQKLPAEYGTAFITGHSNGSVRIYDASHGDIQDNASFEVNLSRTLNKAKELAVDKISFAAETLELAVSIETGDVVLFKYEVNQFYSVENRPESGDLEMNFRRFSLNNTNGVLVDVRDRAPTGVRQGFMPSTAVHANKGKTSAINNSNIGFVGIAYAAGSLMLIDRRGPAIIYMENIREISGAQSACVTCIEFVIMEYGDDGYSSILMVCGTDMGEVITYKILPASGGKFDVQLMDITNVTSKGPIHKIDAFSKETKSSCLATIPKMQNLSKGLCIPGIVLITGFDDIRLITLGKSKSTHKGFKYPLAATGLSYISTVEKNNDRKNLTVIITLEINGHLRVFTIPDFKEQMSEHIPFPIAAKYITESSVLRNGDIAIRVSEFQASLFSTVKEQDTLAPVSDTLYINGIRIPYRPQVNSLQWARGTVYCTPAQLNELLGGVNRPASKYKESIIAEGSFSERSSDDNNANHPEHQYTKPTRKGRNSSYGVLRNVSRAVETRWDAVEDRFNDYATAMGETMNEAVEQTGKDVMKGALGF FFB0901883009815 sni1p-fir1p-1 3145122 N/A 3145124 unspecified role unspecified role prey prey Region 173-876 Region 173-876 sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3145125 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false sni1p-fir1p-2 3145123 N/A 3145124 unspecified role unspecified role prey prey Region 173-876 Region 173-876 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3145125 unspecified role unspecified role bait bait Region 41-99 Region 41-99 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false