DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ schwer-2006-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Schwer B., Bunkenborg J., Verdin RO., Andersen JS., Verdin E. DIP original experiment: DIP-263311X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2. Schwer B., Bunkenborg J., Verdin RO., Andersen JS., Verdin E. Only protein-protein interactions imex curation schwer-2006-2 in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Schwer B., Bunkenborg J., Verdin RO., Andersen JS., Verdin E. DIP original experiment: DIP-263312X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2. Schwer B., Bunkenborg J., Verdin RO., Andersen JS., Verdin E. Only protein-protein interactions imex curation sir3_human NAD-dependent protein deacetylase sirtuin-3, mitochondrial SIRT3 SIR2L3 SIR2-like protein 3 Regulatory protein SIR2 homolog 3 protein protein human Homo sapiens Human MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK q9nub1-1 Acetyl-coenzyme A synthetase 2-like, mitochondrial ACSS1 ACAS2L KIAA1846 Acetate--CoA ligase 2 Acetyl-CoA synthetase 2 Acyl-CoA synthetase short-chain family member 1 Propionate--CoA ligase protein protein human Homo sapiens Human MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK q9ntg7-1 NAD-dependent protein deacetylase sirtuin-3, mitochondrial SIRT3 SIR2L3 Regulatory protein SIR2 homolog 3 SIR2-like protein 3 protein protein human Homo sapiens Human MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK acss1-sirt3-1 3145108 N/A 3145110 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3145111 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false acss1-sirt3-2 3145109 N/A 3145112 enzyme enzyme unspecified role unspecified role Q9NTG7-1:p.His248Tyr H248Y mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Region 102-399 Region 102-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3145111 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli deacetylation deacetylation reaction deacetylation false false false