DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dong-2006-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. DIP original experiment: DIP-263160X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1. Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. Only protein-protein interactions imex curation dong-2006-3 in vitro In vitro pull down pull down autoradiography autoradiography Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. DIP original experiment: DIP-263158X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1. Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. Only protein-protein interactions imex curation dong-2006-4 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. DIP original experiment: DIP-263161X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1. Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. Only protein-protein interactions imex curation dong-2006-5 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. DIP original experiment: DIP-263159X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1. Dong CH., Agarwal M., Zhang Y., Xie Q., Zhu JK. Only protein-protein interactions imex curation ice1_arath Transcription factor ICE1 SCRM BHLH116 EN45 ICE1 Basic helix-loop-helix protein 116 Inducer of CBF expression 1 Transcription factor EN 45 Transcription factor SCREAM bHLH transcription factor bHLH116 MDJ14.1 MLJ15.14 At3g26744 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGLDGNNGGGVWLNGGGGEREENEEGSWGRNQEDGSSQFKPMLEGDWFSSNQPHPQDLQMLQNQPDFRYFGGFPFNPNDNLLLQHSIDSSSSCSPSQAFSLDPSQQNQFLSTNNNKGCLLNVPSSANPFDNAFEFGSESGFLNQIHAPISMGFGSLTQLGNRDLSSVPDFLSARSLLAPESNNNNTMLCGGFTAPLELEGFGSPANGGFVGNRAKVLKPLEVLASSGAQPTLFQKRAAMRQSSGSKMGNSESSGMRRFSDDGDMDETGIEVSGLNYESDEINESGKAAESVQIGGGGKGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPGSLPPTSSSFHPLTPTPQTLSCRVKEELCPSSLPSPKGQQARVEVRLREGRAVNIHMFCGRRPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAGYAGMI hos1_arath E3 ubiquitin-protein ligase HOS1 HOS1 Protein HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 RING finger protein HOS1 RING-type E3 ubiquitin transferase HOS1 T5I7.11 At2g39810 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDTREINGFASAARSISLPTQPNYSSKPVQEALKHLASINLRELCNEAKVERCRATRDLASCGRFVNYVLNPCGHASLCTECCQRCDVCPICRSTLPKFGDRLRLRLYYECVEAGLISRTHEEASQDSDEDEHQLAADVHRLYSLFDVAMNNNLISVVCHYITNVCMDETAVSSDPVIAFLLDEVVVKDWVKRTFRSTLAELQEIYNLETKEMQAWLDKLLRCSKQVAGICSVLEVMESAFKGSVSPQLQDVQTLRENIGKTKQHLDIMVWCIRHGFLDDVRSRYSNFTSWNALVGERKSNAVKRAWPDAVDQSSDCSVQSASLFIEDALENLEREPEYSQEIGADLEVGRLQKDKRSFLRSKIEGTSGSYPFENLRTAADMLFLHGGSDLVVAKQAIFLYYLFDRHWTTPEKYWKHTIDDFAATFGITRHSLLESFVFYLLDDHSEEALQEACRILPEICGPETYPKVAQVLLERDNPETALMVLRWSGRDGVSELVSIGEAVTALRVRVECGLLSEAFTYQRTLCLKVKENNLKNGAVKHASDDLDIWSWTEWMEILVNEFCCLSIRRNLVDRIIELPWNPDEEKYLHRCLLDSATDDPSSAVGSLLVVFYLQRYRYIQAYQVDLRLQKIEEAFVSDNQIGEEVMFRMRSQSHWRKELVDRAIDILPVIQQQQVRSGQFSEMEDASEGAKKSDLPDAPDMITSSVPFATTNSVFLQSANNARAREPVANNGSPFQPGHMIGNASHDLSHGRLFTNANRGQKSEVRSVTKNLKFGEMSTPFKDLNRARGNSQLQGKRTEESSPEVNVDRYIENNMSSPYLRRITANNPVTVKSSSNHLNGSSQKPESTFFGTRMQPDKDNFVDLDDPMDMSSSLKDNNNNVLATESRNNSGGLRWRSDETSDDEDELTSFGSMPVKGRRRRRFAAR ubiq_arath Ubiquitin UBQ1 UBQ2 UBQ3 UBQ4 UBQ5 UBQ6 UBQ7 UBQ10 UBQ11 UBQ14 UBQ15 UBQ16 RPL40B RPL40A RPS27AC RPS27AB RUB2 RUB1 RPS27AA F3C22.8 F22O6_30 F9C22.10 F15A17_270 MOK16.15 T1M15.20 T17J13.210 F14M4.6 T20F21.24 C17L7.240 T32N4.13 T5J8.21 T19E23.13 F28C11.5 F26F24.28 At3g52590 At2g36170 At5g03240 At5g20620 At3g62250 At2g47110 At2g35635 At4g05320 At4g05050 At4g02890 At1g31340 At1g23410 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG hos1-scrm-1 3144232 N/A 3144236 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3144237 unspecified role unspecified role bait bait Region 450-842 Region 450-842 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hos1-scrm-2 3144233 N/A 3144236 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3144237 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false scrm-ub-1 3144234 N/A 3144236 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3144238 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false hos1-scrm-3 3144235 N/A 3144236 unspecified role unspecified role unspecified role unspecified role nYFP-tag nYFP-tag nyfp nYFP n-term range n-terminal range n-term range n-term range n-terminal range n-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3144237 unspecified role unspecified role unspecified role unspecified role cYFP-tag cYFP-tag cyfp cYFP n-term range n-terminal range n-term range n-term range n-terminal range n-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false