DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ paschos-2006-1 in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262894X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions imex curation paschos-2006-2 in vitro In vitro bn-page blue native page bn-page predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262897X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions imex curation paschos-2006-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262895X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions imex curation paschos-2006-4 in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262898X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions imex curation paschos-2006-5 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262896X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions imex curation virb8_brusu Type IV secretion system protein virB8 BRA0062 BS1330_II0062 virB8 protein protein brusu brusu MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNAQTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVRTSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ virb4_brusu Type IV secretion system protein virB4 virB4 BRA0066 BS1330_II0066 protein protein brusu brusu MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLIRKDQLNTLFRAIASNNVALWSHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRKLDEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEIRTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAIDQLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLSCFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHNAPMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAIRTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDNADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQQVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQVIAEVGDDPSVWLPVFQERRKARIASSKSTGR virba_brusu Type IV secretion system protein virB10 BS1330_II0060 virB10 BRA0060 protein protein brusu brusu MTQENIPVQPGTLDGERGLPTVNENGSGRTRKVLLFLFVVGFIVVLLLLLVFHMRGNAENNHHSDKTMVQTSTVPMRTFKLPPPPPPPPPAPPEPPAPPPAPAMPIAEPAAAALSLPPLPDDTPAKDDVLDKSASALMVVTKSSGDTNAQTAGDTVVQTTNARIQALLDSQKNTKQDAGSLGTLLHGTQTDARMASLLRNRDFLLAKGSIINCALQTRLDSTVPGMAACVVTRNMYSDNGKVLLIERGSTISGEYDANVKQGMARIYVLWTRVKTPNGVVIDLDSPGADPLGGAGLPGYIDSHFWKRFGGALMLSTIETLGRYATQKVGGGGSNQINLNTGGGESTSNLASTALKDTINIPPTLYKNQGEEIGIYIARDLDFSSVYDVKPK mint virb8-3 3143889 N/A 3143894 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false virb4-virb8-1 3143890 N/A 3143895 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3143894 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Arg230Asp R230D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false virb8-4 3143891 N/A 3143894 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Met102Arg M102R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q7CEG3:p.Tyr105Arg Y105R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q7CEG3:p.Glu214Arg E214R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false virb10-virb8-4 3143892 N/A 3143896 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3143894 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Thr201Tyr T201Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q7CEG3:p.Thr201Ala T201A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false virb8-5 3143893 N/A 3143894 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Met102Arg M102R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q7CEG3:p.Tyr105Arg Y105R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q7CEG3:p.Glu214Arg E214R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false