DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ darios-2006-1 rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Darios F., Davletov B. DIP original experiment: DIP-255196X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Darios F., Davletov B. Only protein-protein interactions imex curation darios-2006-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Darios F., Davletov B. DIP original experiment: DIP-255194X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Darios F., Davletov B. Only protein-protein interactions imex curation darios-2006-3 rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Darios F., Davletov B. DIP original experiment: DIP-255197X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Darios F., Davletov B. Only protein-protein interactions imex curation darios-2006-4 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Darios F., Davletov B. DIP original experiment: DIP-255195X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Darios F., Davletov B. Only protein-protein interactions imex curation stx3_rat Syntaxin-3 Stx3 Stx3a protein protein rat Rattus norvegicus (Rat) Rat MKDRLEQLKAKQLTQDDDTDEVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKRAMKYQGQARKKLIIIIVIVVVLLGILALIIGLSVGLK mint Potentially involved in docking of synaptic vesicles at presynaptic active zones snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG stx3-snap25-1 3143319 N/A 3143323 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat N/A 3143324 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat physical association physical association false false false snap25-stx3-1 3143320 N/A 3143323 unspecified role unspecified role unspecified role unspecified role Region 1-261 Region 1-261 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3143324 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false snap25-stx3-2 3143321 N/A 3143323 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3143324 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat physical association physical association false false false stx3-snap25-2 3143322 N/A 3143323 unspecified role unspecified role bait bait Region 1-261 Region 1-261 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3143324 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false