DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hanson-2006-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262725X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-4 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262739X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-5 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262734X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-6 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262730X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-7 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262740X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-8 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262726X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-9 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262736X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-10 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262735X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-11 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262731X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-12 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262732X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-13 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262727X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-14 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262737X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-15 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262741X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-16 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262728X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-17 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262738X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-18 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262733X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation hanson-2006-19 in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262729X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions imex curation opsd_bovin Rhodopsin RHO protein protein bovin Bos taurus (Bovine) Bovine MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA mint Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal p08168-1 S-arrestin SAG 48 kDa protein Retinal S-antigen Rod photoreceptor arrestin protein protein bovin Bos taurus (Bovine) Bovine MKANKPAPNHVIFKKISRDKSVTIYLGKRDYIDHVERVEPVDGVVLVDPELVKGKRVYVSLTCAFRYGQEDIDVMGLSFRRDLYFSQVQVFPPVGASGATTRLQESLIKKLGANTYPFLLTFPDYLPCSVMLQPAPQDVGKSCGVDFEIKAFATHSTDVEEDKIPKKSSVRLLIRKVQHAPRDMGPQPRAEASWQFFMSDKPLRLAVSLSKEIYYHGEPIPVTVAVTNSTEKTVKKIKVLVEQVTNVVLYSSDYYIKTVAAEEAQEKVPPNSSLTKTLTLVPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDKTVMGILVSYQIKVKLTVSGLLGELTSSEVATEVPFRLMHPQPEDPDTAKESFQDENFVFEEFARQNLKDAGEYKEEKTDQEAAMDE rho-sag-1 3142681 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role P08168-1:p.Leu77Cys L77C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P08168-1:p.Phe79Cys F79C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Region 1-378 Region 1-378 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false rho-sag-2 3142682 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false Region 1-378 Region 1-378 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-3 3142683 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-4 3142684 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-5 3142685 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-6 3142686 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-7 3142687 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-8 3142688 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-9 3142689 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-10 3142690 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-11 3142691 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-12 3142692 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-13 3142693 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-14 3142694 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-15 3142695 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-16 3142696 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rho-sag-17 3142697 N/A 3142698 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3142699 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false