DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ nayak-2006-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip anti tag western anti tag western blot anti tag western Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. DIP original experiment: DIP-66153X Structure (London, England : 1993) 2006 IMEx Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family. Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. Only protein-protein interactions imex curation nayak-2006-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. DIP original experiment: DIP-66155X Structure (London, England : 1993) 2006 IMEx Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family. Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. Only protein-protein interactions imex curation nayak-2006-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. DIP original experiment: DIP-66154X Structure (London, England : 1993) 2006 IMEx Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family. Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. Only protein-protein interactions imex curation nayak-2006-4 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. DIP original experiment: DIP-66152X Structure (London, England : 1993) 2006 IMEx Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family. Nayak V., Zhao K., Wyce A., Schwartz MF., Lo WS., Berger SL., Marmorstein R. Only protein-protein interactions imex curation snf1_yeast Carbon catabolite-derepressing protein kinase SNF1 CAT1 CCR1 GLC2 PAS14 Sucrose nonfermentating protein 1 D8035.20 YDR477W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNNNTNTAPANANSSHHHHHHHHHHHHHGHGGSNSTLNNPKSSLADGAHIGNYQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKSDMQGRIEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYAGNELFDYIVQRDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLLDEHLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFDDESIPVLFKNISNGVYTLPKFLSPGAAGLIKRMLIVNPLNRISIHEIMQDDWFKVDLPEYLLPPDLKPHPEEENENNDSKKDGSSPDNDEIDDNLVNILSSTMGYEKDEIYESLESSEDTPAFNEIRDAYMLIKENKSLIKDMKANKSVSDELDTFLSQSPPTFQQQSKSHQKSQVDHETAKQHARRMASAITQQRTYHQSPFMDQYKEEDSTVSILPTSLPQIHRANMLAQGSPAASKISPLVTKKSKTRWHFGIRSRSYPLDVMGEIYIALKNLGAEWAKPSEEDLWTIKLRWKYDIGNKTNTNEKIPDLMKMVIQLFQIETNNYLVDFKFDGWESSYGDDTTVSNISEDEMSTFSAYPFLHLTTKLIMELAVNSQSN F5C63565C986C4E3 snf1-snf1-1 3142224 N/A 3142228 unspecified role unspecified role prey prey Ha-tag Ha-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3142228 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false snf1-snf1-2 3142225 N/A 3142228 unspecified role unspecified role bait bait Ha-tag Ha-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3142228 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false snf1-1 3142226 N/A 3142228 unspecified role unspecified role unspecified role unspecified role Region 33-320 Region 33-320 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false snf1-2 3142227 N/A 3142228 unspecified role unspecified role unspecified role unspecified role Region 33-320 Region 33-320 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false