DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ deng-2006-3 ecolx Escherichia coli cd circular dichroism CD CD cd predetermined predetermined participant predetermined Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. DIP original experiment: DIP-66145X Structure (London, England : 1993) 2006 IMEx Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. Only protein-protein interactions imex curation deng-2006-4 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. DIP original experiment: DIP-66142X Structure (London, England : 1993) 2006 IMEx Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. Only protein-protein interactions imex curation deng-2006-5 ecolx Escherichia coli cd circular dichroism CD CD cd predetermined predetermined participant predetermined Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. DIP original experiment: DIP-66143X Structure (London, England : 1993) 2006 IMEx Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. Only protein-protein interactions imex curation deng-2006-6 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. DIP original experiment: DIP-66144X Structure (London, England : 1993) 2006 IMEx Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Deng Y., Liu J., Zheng Q., Eliezer D., Kallenbach NR., Lu M. Only protein-protein interactions imex curation gcn4_yeast General control transcription factor GCN4 ARG9 GCN4 AAS3 Amino acid biosynthesis regulatory protein AAS101 General control protein GCN4 YEL009C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER 2ED1B8E35D509578 gcn4-1 3141245 N/A 3141249 unspecified role unspecified role unspecified role unspecified role Region 251-281 Region 251-281 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys256Val K256V mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys263Val K263V mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Glu270Val E270V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gcn4-2 3141246 N/A 3141249 unspecified role unspecified role unspecified role unspecified role Region 251-281 Region 251-281 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys256Ala K256A mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys263Ala K263A mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Glu270Ala E270A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gcn4-3 3141247 N/A 3141249 unspecified role unspecified role unspecified role unspecified role Region 251-281 Region 251-281 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys256Ala K256A mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys263Ala K263A mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Glu270Ala E270A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gcn4-4 3141248 N/A 3141249 unspecified role unspecified role unspecified role unspecified role Region 251-281 Region 251-281 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys256Val K256V mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Lys263Val K263V mutation mutation certain certain sequence position certain certain certain sequence position certain false P03069:p.Glu270Val E270V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false