DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hirano-2006-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66178X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions imex curation hirano-2006-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66179X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions imex curation hirano-2006-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66180X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions imex curation hirano-2006-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66181X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions imex curation hgs_human Hepatocyte growth factor-regulated tyrosine kinase substrate Protein pp110 Hrs HGS HRS protein protein human Homo sapiens Human MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKDLLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPSGGPFSEPQFHNGESEESHEQFLKALQNAVTTFVNRMKSNHMRGRSITNDSAVLSLFQSINGMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPAAGGVLYQPSGPASFPSTFSPAGSVEGSPMHGVYMSQPAPAAGPYPSMPSTAADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAGYQNVASQAPQSLPAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPSQDASLPPQQPYIAGQQPMYQQMAPSGGPPQQQPPVAQQPQAQGPPAQGSEAQLISFD ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG dnjb2_human DnaJ homolog subfamily B member 2 DNAJB2 HSJ1 HSPF3 Heat shock 40 kDa protein 3 Heat shock protein J1 protein protein human Homo sapiens Human MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL eps15_mouse Epidermal growth factor receptor substrate 15 Protein AF-1p Eps15 protein protein mouse Mus musculus Mouse MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLKKSGLPDLILGKIWDLADTDGKGVLSKQEFFVALRLVACAQNGLEVSLSSLSLAVPPPRFHDSSSPLLTSGPSVAELPWAVKSEDKAKYDAIFDSLSPVDGFLSGDKVKPVLLNSKLPVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSALLAHIWSLCDTKGCGKLSKDQFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNITGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTVKQRTSEVQDLQDEVQRESINLQKLQAQKQQVQELLGELDEQKAQLEEQLQEVRKKCAEEAQLISSLKAEITSQESQISSYEEELLKAREELSRLQQETAQLEESVESGKAQLEPLQQHLQESQQEISSMQMRLEMKDLETDNNQSNWSSSPQSVLVNGATDYCSLSTSSSETANFNEHAEGQNNLESEPTHQESSVRSSPEIAPSDVTDESEAVTVAGNEKVTPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFTTSSTDPFSASSNSSNTSVETWKHNDPFAPGGTVVAAASDSATDPFASVFGNESFGDGFADFSTLSKVNNEDAFNPTISSSTSSVTIAKPMLEETASKSEDVPPALPPKVGTPTRPCPPPPGKRPINKLDSSDPLKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA hgs-ubiquitin-1 3141176 N/A 3141180 unspecified role unspecified role neutral component neutral component 257-277 binding region 257-277 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3141181 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false hgs-ubiquitin-2 3141177 N/A 3141180 unspecified role unspecified role bait bait 251-286 binding region 251-286 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false O14964:p.[Ala266Gln;Ala268Gln] A266Q, A268Q mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141181 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false dnajb2-ubiquitin-1 3141178 N/A 3141182 unspecified role unspecified role bait bait 245-272 binding region 245-272 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false P25686:p.[Ala258Gln;Ala260Gln] A258Q, A260Q mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141181 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false eps15-ubiquitin-1 3141179 N/A 3141183 unspecified role unspecified role bait bait 867-897 binding region 867-897 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false P42567:p.[Ala886Gln;Ala888Gln] A886Q, A888Q mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141181 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false