DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2006-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66207X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation lee-2006-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66208X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation lee-2006-6 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66209X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation lee-2006-7 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66210X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation lee-2006-8 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66211X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation lee-2006-9 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66212X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S., Tsai YC., Mattera R., Smith WJ., Kostelansky MS., Weissman AM., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation rabx5_bovin Rab5 GDP/GTP exchange factor Rabex-5 RABGEF1 RABEX5 protein protein bovin Bos taurus (Bovine) Bovine MSLKSERRGIHVDQSELLCKKGCGYYGNPAWQGFCSKCWREEYHKARQKQIQEDWELAERLQREEEEAFASSQSSQGAQSLTFSKFEEKKTNEKTRKVTTVKKFFSASSRVGAKKAEIQEAKAPSPSINRQTSIETDRVSKEFIEFLKTFHKTGQEIYKQTKLFLEAMHYKRDLSIEEQSECTQDFYQNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALHWVTPQMLCVPVNEEIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQESENWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAKEVQDIVEKYPLEIRPPNQSVAAIDSENVENDKLPPPLQPQVYAG ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub2g2_mouse Ubiquitin-conjugating enzyme E2 G2 Ube2g2 Ubiquitin carrier protein G2 Ubiquitin-protein ligase G2 Ubc7 E2 ubiquitin-conjugating enzyme G2 protein protein mouse Mus musculus Mouse MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS p61077-1 Ubiquitin-conjugating enzyme E2 D3 UBE2D3 UBC5C UBCH5C (E3-independent) E2 ubiquitin-conjugating enzyme D3 E2 ubiquitin-conjugating enzyme D3 Ubiquitin carrier protein D3 Ubiquitin-conjugating enzyme E2(17)KB 3 Ubiquitin-conjugating enzyme E2-17 kDa 3 Ubiquitin-protein ligase D3 protein protein human Homo sapiens Human MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM rnf25_human E3 ubiquitin-protein ligase RNF25 RNF25 RING finger protein 25 RING-type E3 ubiquitin transferase RNF25 protein protein human Homo sapiens Human MAASASAAAGEEDWVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVCFTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQHICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS rabgef1-ub-8 3141123 N/A 3141129 unspecified role unspecified role neutral component neutral component 9-73 binding region 9-73 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3141130 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false rabgef1-ub-9 3141124 N/A 3141129 unspecified role unspecified role neutral component neutral component 9-73 binding region 9-73 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false O18973:p.Tyr26Ala Y26A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.Ala58Asp A58D mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.[Tyr25Ala;Ala58Asp] Y25A, A58D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3141130 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false rabgef1-ub-10 3141125 N/A 3141129 unspecified role unspecified role neutral component neutral component O18973:p.Tyr26Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false 9-73 binding region 9-73 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false O18973:p.Tyr25Ala Y25A, Y25P mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.Tyr26Ala Y26A, Y26P mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.Ile51Asp I51D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.Leu57Asp L57D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.Ala58Asp A58D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O18973:p.[Tyr25Ala;Leu57Asp] Y25A, L57D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O18973:p.[Tyr25Ala;Ala58Asp] Y25A, A58D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O18973:p.[Tyr26Ala;Leu57Asp] Y26A, L57D mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O18973:p.Tyr25Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3141130 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false amfr-ube2g2-11 3141126 N/A 3141131 unspecified role unspecified role prey prey in vitro In vitro N/A 3141132 unspecified role unspecified role bait bait 574-643 binding site 574-643 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false GST GST gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rabgef1-ube2d3-1 3141127 N/A 3141133 unspecified role unspecified role prey prey in vitro In vitro N/A 3141129 unspecified role unspecified role bait bait 9-73 binding site 9-73 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false O18973:p.[Tyr25Ala;Tyr26Ala] Y25A, Y26A mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false rnf25-ube2d3-1 3141128 N/A 3141134 unspecified role unspecified role bait bait 86-363 binding site 86-363 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141133 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction false false false