DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chen-2006-11 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262481X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-12 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262487X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-13 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262484X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-14 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262488X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-15 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262482X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-16 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262486X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-17 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262490X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-18 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262489X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-19 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262483X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation chen-2006-20 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. DIP original experiment: DIP-262485X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B., Mariano J., Tsai YC., Chan AH., Cohen M., Weissman AM. Only protein-protein interactions imex curation cd3d_human T-cell surface glycoprotein CD3 delta chain CD3D T3D T-cell receptor T3 delta chain protein protein human Homo sapiens Human MEHSTFLSGLVLATLLSQVSPFKIPIEELEDRVFVNCNTSITWVEGTVGTLLSDITRLDLGKRILDPRGIYRCNGTDIYKDKESTVQVHYRMCQSCVELDPATVAGIIVTDVIATLLLALGVFCFAGHETGRLSGAADTQALLRNDQVYQPLRDRDDAQYSHLGGNWARNK amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG ub2g2_mouse Ubiquitin-conjugating enzyme E2 G2 Ube2g2 Ubiquitin carrier protein G2 Ubiquitin-protein ligase G2 Ubc7 E2 ubiquitin-conjugating enzyme G2 protein protein mouse Mus musculus Mouse MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL ub2g2_human Ubiquitin-conjugating enzyme E2 G2 Ubiquitin-protein ligase G2 Ubiquitin carrier protein G2 UBE2G2 E2 ubiquitin-conjugating enzyme G2 UBC7 protein protein human Homo sapiens Human MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tera_mouse Transitional endoplasmic reticulum ATPase Vcp 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein mouse Mus musculus Mouse MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG amfr-cd3d-1 3140404 N/A 3140414 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3140415 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false amfr-vcp-2 3140405 N/A 3140416 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3140415 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ube2g2-amfr-1 3140406 N/A 3140417 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3140415 unspecified role unspecified role prey prey Region 309-611 Region 309-611 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false gln_arg_met_leu_val_gln579-584ala_ala_ala_gly_gly_gly Q574A/R580A/M581A/L582G/V583G/Q584G required to bind necessary binding region required to bind substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false amfr-ube2g2-8 3140407 N/A 3140418 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3140415 unspecified role unspecified role bait bait Region 574-611 Region 574-611 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false amfr-hub-1 3140408 N/A 3140419 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3140415 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false amfr-vcp-3 3140409 N/A 3140420 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3140415 unspecified role unspecified role bait bait Region 574-643 Region 574-643 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false ube2g2-amfr-2 3140410 N/A 3140417 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3140415 unspecified role unspecified role prey prey Region 574-611 Region 574-611 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Gln579_Gln584delinsAlaAlaAlaGlyGlyGly Q574A/R580A/M581A/L582G/V583G/Q584G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false amfr-ube2g2-9 3140411 N/A 3140415 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 574-643 Region 574-643 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Gln579_Gln584delinsAlaAlaAlaGlyGlyGly Q574A/R580A/M581A/L582G/V583G/Q584G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3140417 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false cd3d-hub-1 3140412 N/A 3140414 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3140419 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false amfr-ube2g2-10 3140413 N/A 3140415 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 574-611 Region 574-611 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Gln579_Gln584delinsAlaAlaAlaGlyGlyGly Q574A/R580A/M581A/L582G/V583G/Q584G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3140417 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false