DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hansen-2006-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hansen DV., Tung JJ., Jackson PK. DIP original experiment: DIP-262496X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. Hansen DV., Tung JJ., Jackson PK. Only protein-protein interactions imex curation hansen-2006-2 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hansen DV., Tung JJ., Jackson PK. DIP original experiment: DIP-262497X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. Hansen DV., Tung JJ., Jackson PK. Only protein-protein interactions imex curation fbx43_xenla F-box only protein 43 fbxo43 emi2 erp1 fbx26 Early mitotic inhibitor 2 Emi1-related protein 1 F-box protein 26 Xerp1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MANLLENFAAHHSMTAGAKKKADHQDTSVSQDSGYSDSLKGFSPDSHKSGNFLETVTEGYENSENIDPTLILSPIKYELSWGADTRESKQLAPLYETPRVGKKEFSLRRRLLISKATSGGNLDFDVSVCSAESCGREKSLRRIPSHEGSLSNSFADSPRDGSYEPIATSTLKTESESGTSCKKWRLSFAQQRSSTLDDSKSDSIPLPEVENISPVQHSLASSTDDSILYEETIFGAPTTPTCNFIVKEEFQTPISNLAANFRFNLCTPDVGHVSDFDISVTEDSAFHSLSLDKSQDSITDHEGSFQELIQKPRETSKAVNNKSRLRKLDRCRRLSTLRERGSQSEVEEEGNEVPVLSSAYKLKVARASVDEENEFSSDESRVHSLLSSDDLTGKPALRVLHEMLLRSTRKRPQQATVQDLLGSSGCFELPEDSLSRLIGRKMGLETFDILAELKNRNLKHILASILDLVNAASICSMCRVSRDWRDVVLQDKSAHQRRKAYIKKLKTEAEQGRQLSFEDSATRLNILSRSALRSVQIQARSAFRTPTSSLTPGDNKSIHSASKHQEYLKVAKTLFTDEALKPCPRCQYPAKYQALKKRGTCSRKDCGFDFCSLCLCTFHGSKECGTGSAKRIPKKEALPGSAQSKRNLKRL mint plk1_xenla Serine/threonine-protein kinase PLK1 plx1 Plx1 Polo-like kinase 1 plk1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAQVAGKKLTVAPEAAKPPGIPGSSSAVKEIPEILVDPRTRRRYLRGRFLGKGGFAKCYEITDLESREVFAGKIVPKTMLLKPHQKDKMTMEIAIQRSLDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEPEARYYLKQTISGCQYLHSNRVIHRDLKLGNLFLNDEMEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRIKKNEYSIPKHINPVAAALIQKMLRSDPTSRPTIDDLLNDEFFTSGYIPSRLPTTCLTVPPRFSIAPSTIDQSLRKPLTAINKGQDSPLVEKQVAPAKEEEMQQPEFTEPADCYLSEMLQQLTCLNAVKPSERALIRQEEAEDPASIPIFWISKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLIMYNDGDSLQYIERNNTESYLNVRSYPTTLTKKITLLKYFRNYMSEHLLKAGANTTPREGDELARLPFLRTWFRTRSAIILHLSNGTVQINFFQDHTKIILCPLMAAVSYIDEKREFRTYKLSLIQEFGCCKELASRLRYARTMVEKLQSSKSAVAHVKASA plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS fbxo43-plk1-1 3140295 N/A 3140297 unspecified role unspecified role bait bait Region 1-350 Region 1-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3140298 unspecified role unspecified role prey prey xenla Xenopus laevis (African clawed frog) African clawed frog physical association physical association false false false fbxo43-plk1-2 3140296 N/A 3140297 unspecified role unspecified role bait bait Region 1-350 Region 1-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3140299 unspecified role unspecified role prey prey Region 326-603 Region 326-603 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false