DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ eletr-2005-1 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Eletr ZM., Huang DT., Duda DM., Schulman BA., Kuhlman B. DIP original experiment: DIP-255134X Nature structural & molecular biology 2005 IMEx E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer. Eletr ZM., Huang DT., Duda DM., Schulman BA., Kuhlman B. Only protein-protein interactions imex curation q05086-2 Ubiquitin-protein ligase E3A E6AP E6AP ubiquitin-protein ligase EPVE6AP HPVE6A Human papillomavirus E6-associated protein Oncogenic protein-associated protein E6-AP Renal carcinoma antigen NY-REN-54 UBE3A HECT-type ubiquitin transferase E3A protein protein human Homo sapiens Human MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML Variant in position: 212:D->V (in AS, unknown pathological significance). Variant in position: 237:L->Q (in AS, unknown pathological significance). Variant in position: 263:L->W (in AS, unknown pathological significance). Variant in position: 458:P->L (in AS, unknown pathological significance). Variant in position: 545:G->R (in AS, unknown pathological significance). Variant in position: 584:E->Q (in AS, unknown pathological significance). Variant in position: 656:T->I (in AS, unknown pathological significance). Variant in position: 690:F->C (in AS, unknown pathological significance). Variant in position: 827:P->L (in AS, unknown pathological significance). Variant in position: 106:T->K (in AS, unknown pathological significance). Variant in position: 237:L->H (in AS, unknown pathological significance). Variant in position: 435:L->P (in AS, unknown pathological significance). Variant in position: 477:R->P (in AS, unknown pathological significance). Variant in position: 566:M->K (in AS, unknown pathological significance). Variant in position: 117:C->R (common polymorphism, may be associated with AS). Variant in position: 270:N->T (common polymorphism, may be associated with AS). Variant in position: 762:V->I (common polymorphism, may be associated with AS). Variant in position: 133:V->G (common polymorphism, may be associated with AS). Variant in position: 335:S->T (common polymorphism, may be associated with AS). Variant in position: 478:M->I (common polymorphism, may be associated with AS). Variant in position: 588:Q->E (common polymorphism, may be associated with AS). Variant in position: 588:Q->P (common polymorphism, may be associated with AS). Variant in position: 673:L->R (common polymorphism, may be associated with AS). p68036-1 Ubiquitin-conjugating enzyme E2 L3 E2 ubiquitin-conjugating enzyme L3 L-UBC UbcH7 Ubiquitin carrier protein L3 Ubiquitin-conjugating enzyme E2-F1 Ubiquitin-protein ligase L3 UBE2L3 UBCE7 UBCH7 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD ube2l3-ube3a-2 3138762 N/A 3138763 unspecified role unspecified role unspecified role unspecified role Q05086-2:p.Cys820Ala C820A mutation mutation certain certain sequence position certain certain certain sequence position certain false HCET domain Region 492-852 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3138764 unspecified role unspecified role unspecified role unspecified role P68036-1:p.Phe63Asp F63D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false BODIPY-label BODIPY-label bodipy label bodipy label n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false