DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hickman-2005-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254786X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions imex curation hickman-2005-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254783X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions imex curation hickman-2005-3 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254784X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions imex curation hickman-2005-4 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254785X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions imex curation q25442_musdo protein protein musdo Musca domestica House fly MQKMDNLEVKAKINQGLYKITPRHKGTSFIWNVLADIQKEDDTLVEGWVFCRKCEKVLKYTTRQTSNLCRHKCCASLKQSRELKTVSADCKKEAIEKCAQWVVRDCRPFSAVSGSGFIDMIKFFIKVGAEYGEHVNVEELLPSPITLSRKVTSDAKEKKALISREIKSAVEKDGASATIDLWTDNYIKRNFLGVTLHYHENNELRDLILGLKSLDFERSTAENIYKKLKAIFLQFNVEDLSSIKFVTDRGANVVKSLANNIRINCSSHLLSNVLENSFEETPELNVPILACKNIVKYFKKANLQHRLRSSLKSECPTRWNSTYTMLRSILDNWESVIQILSEAGETQRIVHINKSIIQTMVNILDGFERIFKELQTCSSPSLCFVVPSILKVKEICSPDVGDVADIAKLKVNIIKNVRIIWEENLSIWHYTAFFFYPPALHMQQEKVAQIKEFCLSKMEDLELINRMSSFNELSATQLNQSDSNSHNSIDLTSHSKDISTTSFFFPQLTQNNSREPPVCPSDEFEFYRKEIVILSEDFKVMEWWNLNSKKYPKLSKLALSLLSIPASSAASERTFSLAGNIITEKRNRIGQQTVDSLLFLNSFYKNFCKLDI q25442_musdo-q25442_musdo-1 3138579 N/A 3138583 unspecified role unspecified role unspecified role unspecified role Region 79-162 Region 79-162 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3138583 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false q25442_musdo-1 3138580 N/A 3138583 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q25442:p.[Arg369Ala;Phe503Ala;Phe504Ala] R369A/F503A/F504A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false q25442_musdo-2 3138581 N/A 3138583 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false q25442_musdo-3 3138582 N/A 3138583 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false