DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hostetter-2004-1 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. DIP original experiment: DIP-110957X PLoS biology 2004 IMEx Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. Only protein-protein interactions imex curation hostetter-2004-2 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. DIP original experiment: DIP-110960X PLoS biology 2004 IMEx Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. Only protein-protein interactions imex curation hostetter-2004-3 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. DIP original experiment: DIP-110958X PLoS biology 2004 IMEx Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. Only protein-protein interactions imex curation hostetter-2004-4 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. DIP original experiment: DIP-110959X PLoS biology 2004 IMEx Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. Hostetter D., Rice S., Dean S., Altman D., McMahon PM., Sutton S., Tripathy A., Spudich JA. Only protein-protein interactions imex curation mys2_dicdi Myosin-2 heavy chain mhcA Myosin II heavy chain DDB_G0286355 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MNPIHDRTSDYHKYLKVKQGDSDLFKLTVSDKRYIWYNPDPKERDSYECGEIVSETSDSFTFKTVDGQDRQVKKDDANQRNPIKFDGVEDMSELSYLNEPAVFHNLRVRYNQDLIYTYSGLFLVAVNPFKRIPIYTQEMVDIFKGRRRNEVAPHIFAISDVAYRSMLDDRQNQSLLITGESGAGKTENTKKVIQYLASVAGRNQANGSGVLEQQILQANPILEAFGNAKTTRNNNSSRFGKFIEIQFNSAGFISGASIQSYLLEKSRVVFQSETERNYHIFYQLLAGATAEEKKALHLAGPESFNYLNQSGCVDIKGVSDSEEFKITRQAMDIVGFSQEEQMSIFKIIAGILHLGNIKFEKGAGEGAVLKDKTALNAASTVFGVNPSVLEKALMEPRILAGRDLVAQHLNVEKSSSSRDALVKALYGRLFLWLVKKINNVLCQERKAYFIGVLDISGFEIFKVNSFEQLCINYTNEKLQQFFNHHMFKLEQEEYLKEKINWTFIDFGLDSQATIDLIDGRQPPGILALLDEQSVFPNATDNTLITKLHSHFSKKNAKYEEPRFSKTEFGVTHYAGQVMYEIQDWLEKNKDPLQQDLELCFKDSSDNVVTKLFNDPNIASRAKKGANFITVAAQYKEQLASLMATLETTNPHFVRCIIPNNKQLPAKLEDKVVLDQLRCNGVLEGIRITRKGFPNRIIYADFVKRYYLLAPNVPRDAEDSQKATDAVLKHLNIDPEQYRFGITKIFFRAGQLARIEEAREQRISEIIKAIQAATRGWIARKVYKQAREHTVAARIIQQNLRAYIDFKSWPWWKLFSKARPLLKRRNFEKEIKEKEREILELKSNLTDSTTQKDKLEKSLKDTESNVLDLQRQLKAEKETLKAMYDSKDALEAQKRELEIRVEDMESELDEKKLALENLQNQKRSVEEKVRDLEEELQEEQKLRNTLEKLKKKYEEELEEMKRVNDGQSDTISRLEKIKDELQKEVEELTESFSEESKDKGVLEKTRVRLQSELDDLTVRLDSETKDKSELLRQKKKLEEELKQVQEALAAETAAKLAQEAANKKLQGEYTELNEKFNSEVTARSNVEKSKKTLESQLVAVNNELDEEKKNRDALEKKKKALDAMLEEMKDQLESTGGEKKSLYDLKVKQESDMEALRNQISELQSTIAKLEKIKSTLEGEVARLQGELEAEQLAKSNVEKQKKKVELDLEDKSAQLAEETAAKQALDKLKKKLEQELSEVQTQLSEANNKNVNSDSTNKHLETSFNNLKLELEAEQKAKQALEKKRLGLESELKHVNEQLEEEKKQKESNEKRKVDLEKEVSELKDQIEEEVASKKAVTEAKNKKESELDEIKRQYADVVSSRDKSVEQLKTLQAKNEELRNTAEEAEGQLDRAERSKKKAEFDLEEAVKNLEEETAKKVKAEKAMKKAETDYRSTKSELDDAKNVSSEQYVQIKRLNEELSELRSVLEEADERCNSAIKAKKTAESALESLKDEIDAANNAKAKAERKSKELEVRVAELEESLEDKSGTVNVEFIRKKDAEIDDLRARLDRETESRIKSDEDKKNTRKQFADLEAKVEEAQREVVTIDRLKKKLESDIIDLSTQLDTETKSRIKIEKSKKKLEQTLAERRAAEEGSSKAADEEIRKQVWQEVDELRAQLDSERAALNASEKKIKSLVAEVDEVKEQLEDEILAKDKLVKAKRALEVELEEVRDQLEEEEDSRSELEDSKRRLTTEVEDIKKKYDAEVEQNTKLDEAKKKLTDDVDTLKKQLEDEKKKLNESERAKKRLESENEDFLAKLDAEVKNRSRAEKDRKKYEKDLKDTKYKLNDEAATKTQTEIGAAKLEDQIDELRSKLEQEQAKATQADKSKKTLEGEIDNLRAQIEDEGKIKMRLEKEKRALEGELEELRETVEEAEDSKSEAEQSKRLVELELEDARRNLQKEIDAKEIAEDAKSNLQREIVEAKGRLEEESIARTNSDRSRKRLEAEIDALTAQVDAEQKAKNQQIKENKKIETELKEYRKKFGESEKTKTKEFLVVEKLETDYKRAKKEAADEQQQRLTVENDLRKHLSEISLLKDAIDKLQRDHDKTKRELETETASKIEMQRKMADFFGGFKA mhca-1 3137311 N/A 3137315 unspecified role unspecified role unspecified role unspecified role Region 1531-2116 Region 1531-2116 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false mhca-2 3137312 N/A 3137315 unspecified role unspecified role unspecified role unspecified role Region 1531-2116 Region 1531-2116 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false mhca-3 3137313 N/A 3137315 unspecified role unspecified role unspecified role unspecified role Region 1531-2116 Region 1531-2116 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false mhca-4 3137314 N/A 3137315 unspecified role unspecified role unspecified role unspecified role Region 1531-2116 Region 1531-2116 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false