DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ holaska-2004-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110950X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions imex curation holaska-2004-2 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110955X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions imex curation holaska-2004-3 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110953X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions imex curation holaska-2004-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110951X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions imex curation holaska-2004-5 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110954X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions imex curation holaska-2004-6 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110952X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions imex curation emd_human Emerin EDMD STA EMD protein protein human Homo sapiens Human MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF emd-actb-1 3137273 N/A 3137279 unspecified role unspecified role bait bait Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3137280 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false acta1-emd-1 3137274 N/A 3137281 unspecified role unspecified role bait bait rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3137279 unspecified role unspecified role prey prey Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P50402:p.Gln133His Q133H mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false acta1-emd-2 3137275 N/A 3137281 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3137279 unspecified role unspecified role unspecified role unspecified role Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false emd-actb-2 3137276 N/A 3137279 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3137280 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false acta1-15 3137277 N/A 3137281 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction false false false emd-acta1-1 3137278 N/A 3137281 unspecified role unspecified role prey prey rabit Oryctolagus cuniculus (Rabbit) Rabbit N/A 3137279 unspecified role unspecified role bait bait Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false