NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home gilley-2003-1 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gilley J., Coffer PJ., Ham J. J.Ham@ich.ucl.ac.uk The Journal of cell biology 2003 imex curation tnfl6_human_gene Tumor necrosis factor ligand superfamily member 6 (FASLG) human gene gene gene human Homo sapiens Human foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 bim_human_gene Human Bim gene gene gene human Homo sapiens Human foxo3-faslg-1 3021329 N/A 3021330 unspecified role unspecified role bait bait promoter region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGAATAAGTAAATAAATAA CTAGTTATTTATTTACTTATT region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3021331 unspecified role unspecified role prey prey partially purified partially purified foxo3-DBD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 5b bim_human_gene-foxo3-1 3021329 N/A 3021331 unspecified role unspecified role prey prey partially purified partially purified N-terminal tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false foxo3-DBD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3021332 unspecified role unspecified role bait bait promoter region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGCTAGGGTAAACACGCCG CTAGCGGCGTGTTTACCCTAG CTAGGCAAGGTAAACACTCCT CTAGAGGAGTGTTTACCTTGC mutated promoter region mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 5b O43524 (GO:0001228) up-regulates quantity by expression ENSG00000153094 P01139 (GO:0004871) down-regulates quantity by expression ENSG00000153094 CHEBI:65329 up-regulates quantity by expression ENSG00000153094 foxo3-6 3021329 N/A 3021330 competitor competitor competitor prey prey promoter region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGAATAAGTAAATAAATAA CTAGTTATTTATTTACTTATT N/A 3021330 unspecified role unspecified role bait bait promoter region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGAATAAGTAAATAAATAA CTAGTTATTTATTTACTTATT region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3021331 unspecified role unspecified role prey prey partially purified partially purified foxo3-DBD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 5b_ foxo3-7 3021329 N/A 3021332 competitor competitor competitor prey prey promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGCTAGGGTAAACACGCCG CTAGCGGCGTGTTTACCCTAG N/A 3021330 unspecified role unspecified role bait bait promoter region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGAATAAGTAAATAAATAA CTAGTTATTTATTTACTTATT region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3021331 unspecified role unspecified role prey prey partially purified partially purified foxo3-DBD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 5b_2 bim_human_gene-1 3021329 N/A 3021332 competitor competitor competitor prey prey promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGGCAAGGTAAACACTCCT CTAGAGGAGTGTTTACCTTGC N/A 3021330 unspecified role unspecified role bait bait promoter region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CTAGAATAAGTAAATAAATAA CTAGTTATTTATTTACTTATT region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3021331 unspecified role unspecified role prey prey partially purified partially purified foxo3-DBD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 5b_3