DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ silversmith-2001-1 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262179X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions imex curation silversmith-2001-2 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262176X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions imex curation silversmith-2001-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262177X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions imex curation silversmith-2001-4 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262178X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions imex curation chez_ecoli Protein phosphatase CheZ cheZ b1881 JW1870 Chemotaxis protein CheZ protein protein ecoli Escherichia coli (strain K12) MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEAAEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPIDLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIERQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM chey-chez-3 3135469 N/A 3135473 unspecified role unspecified role unspecified role unspecified role F214C fluorescein label F214C fluorescein label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3135474 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false chey-chez-5 3135470 N/A 3135473 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3135474 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false chez-1 3135471 N/A 3135473 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false chey-chez-6 3135472 N/A 3135474 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3135473 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false