UniProt UniProt http://www.uniprot.org eide-1998-1 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. kjetil.tasken@basalmed.uio.no Experimental cell research 1998 imex curation Only protein-protein interactions eide-1998-2 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. Mitotic cells have been used kjetil.tasken@basalmed.uio.no Experimental cell research 1998 imex curation Only protein-protein interactions eide-1998-3 human-hs68 Homo sapiens Hs68 normal diploid foreskin fibroblasts hs68 Human Hs68 normal diploid foreskin fibroblasts fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. Mitotic cells have been used kjetil.tasken@basalmed.uio.no Experimental cell research 1998 imex curation Only protein-protein interactions kap2_human cAMP-dependent protein kinase type II-alpha regulatory subunit PKR2 PRKAR2 PRKAR2A protein protein human Homo sapiens Human MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ akap8_human A-kinase anchor protein 8 AKAP8 AKAP95 A-kinase anchor protein 95 kDa protein protein human Homo sapiens Human MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE akap8-prkar2a-1 1916190 N/A 1916193 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1916194 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4C prkar2a-akap8-1 1916191 N/A 1916193 unspecified role unspecified role prey prey N/A 1916194 unspecified role unspecified role bait bait physical association physical association false false false 8B akap8-prkar2a-2 1916192 N/A 1916193 unspecified role unspecified role neutral component neutral component N/A 1916194 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 7A