IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ piel-2016-1 mouse-mel745acl.ds19 mouse Mouse mel745acl.ds19 Mouse erythroleukemia cell line transformed by Friend virus strain 745A, clone DS19. Mouse erythroleukemia (MEL) cells were transformed by Friend leukemia virus strain 745A; the clone DS19 is described to be DMSO sensitive, resulting in erythroid differentiation anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Piel RB., Shiferaw MT., Vashisht AA., Marcero JR., Praissman JL., Phillips JD., Wohlschlegel JA., Medlock AE. 27-07-2016: Submitted by A Medlock, Biomedical and Health Sciences Institute, University of Georgia. Subcellular mitochondrial fractions were used in this experiment. UniProtKB identifiers were provided by the author. Enzymatic assay depicted in figure 8 was not curated because the origin of apo-CytB5 could not be ascertained. medlock@uga.edu Biochemistry 2016 Only protein-protein interactions imex curation piel-2016-2 mouse-mel745acl.ds19 mouse Mouse mel745acl.ds19 Mouse erythroleukemia cell line transformed by Friend virus strain 745A, clone DS19. Mouse erythroleukemia (MEL) cells were transformed by Friend leukemia virus strain 745A; the clone DS19 is described to be DMSO sensitive, resulting in erythroid differentiation anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Piel RB., Shiferaw MT., Vashisht AA., Marcero JR., Praissman JL., Phillips JD., Wohlschlegel JA., Medlock AE. 27-07-2016: Submitted by A Medlock, Biomedical and Health Sciences Institute, University of Georgia. Subcellular mitochondrial fractions were used in this experiment. UniProtKB identifiers were provided by the author. Enzymatic assay depicted in figure 8 was not curated because the origin of apo-CytB5 could not be ascertained. medlock@uga.edu Biochemistry 2016 Only protein-protein interactions imex curation piel-2016-5 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Piel RB., Shiferaw MT., Vashisht AA., Marcero JR., Praissman JL., Phillips JD., Wohlschlegel JA., Medlock AE. 27-07-2016: Submitted by A Medlock, Biomedical and Health Sciences Institute, University of Georgia. UniProtKB identifiers were provided by the author. Enzymatic assay depicted in figure 8 was not curated because the origin of apo-CytB5 could not be ascertained. medlock@uga.edu Biochemistry 2016 Only protein-protein interactions imex curation piel-2016-3 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Piel RB., Shiferaw MT., Vashisht AA., Marcero JR., Praissman JL., Phillips JD., Wohlschlegel JA., Medlock AE. 27-07-2016: Submitted by A Medlock, Biomedical and Health Sciences Institute, University of Georgia. Subcellular mitochondrial fractions were used in this experiment. UniProtKB identifiers were provided by the author. Enzymatic assay depicted in figure 8 was not curated because the origin of apo-CytB5 could not be ascertained. medlock@uga.edu Biochemistry 2016 Only protein-protein interactions imex curation piel-2016-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Piel RB., Shiferaw MT., Vashisht AA., Marcero JR., Praissman JL., Phillips JD., Wohlschlegel JA., Medlock AE. 27-07-2016: Submitted by A Medlock, Biomedical and Health Sciences Institute, University of Georgia. UniProtKB identifiers were provided by the author. Enzymatic assay depicted in figure 8 was not curated because the origin of apo-CytB5 could not be ascertained. medlock@uga.edu Biochemistry 2016 Only protein-protein interactions imex curation hemh_mouse Ferrochelatase, mitochondrial Fech Protoheme ferro-lyase Heme synthase protein protein mouse Mus musculus Mouse MLSASANMAAALRAAGALLREPLVHGSSRACQPWRCQSGAAVAATTEKVHHAKTTKPQAQPERRKPKTGILMLNMGGPETLGEVQDFLQRLFLDRDLMTLPIQNKLAPFIAKRRTPKIQERRIGGGSPIKMWTSKQGEGMVKLLDELSPATAPHKYYIGFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNEVGQKPTMKWSTIDRWPTHPLLIQCFADHILKELNHFPEEKRSEVVILFSAHSLPMSVVNRGDPYPQEVGATVHKVMEKLGYPNPYRLVWQSKVGPVPWLGPQTDEAIKGLCERGRKNILLVPIAFTSDHIETLYELDIEYSQVLAQKCGAENIRRAESLNGNPLFSKALADLVHSHIQSNKLCSTQLSLNCPLCVNPVCRKTKSFFTSQQL Catalyzes the ferrous insertion into protoporphyrin IX mint Defects in Fech are the cause of a viable autosomal recessive mutation (named Fechm1Pas or Fch) that causes jaundice and anemia mic27_mouse MICOS complex subunit Mic27 Apolipoprotein O-like Protein FAM121A Apool Fam121a Mic27 protein protein mouse Mus musculus Mouse MAAFRMGKLTTIPAGLIYASINVRLAKEEEPKKQLVRPDQLPIYTAPPLHSKYVEEQPGNLQRGFASIRTTTVYYIGWCKSIYLFMKNGVMDTVQFGKDAYVYLKNPPQDFLPKMGVITASGLAGLLSARKGSRFKKIAYPLGLATLGATVCYPAQSVIIAKITGKKAYATSQQIFQAIKSLWTQKSENESLPEPKEESKEGRSDEIHASLPDLKHSVSLPKELASATVIKSESTSGTTQFIPDPKLMDHGQSHPDDKDMYSTRS hemh_human Ferrochelatase, mitochondrial Protoheme ferro-lyase Heme synthase FECH protein protein human Homo sapiens Human MRSLGANMAAALRAAGVLLRDPLASSSWRVCQPWRWKSGAAAAAVTTETAQHAQGAKPQVQPQKRKPKTGILMLNMGGPETLGDVHDFLLRLFLDRDLMTLPIQNKLAPFIAKRRTPKIQEQYRRIGGGSPIKIWTSKQGEGMVKLLDELSPNTAPHKYYIGFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNQVGRKPTMKWSTIDRWPTHHLLIQCFADHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQEVSATVQKVMERLEYCNPYRLVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVPIAFTSDHIETLYELDIEYSQVLAKECGVENIRRAESLNGNPLFSKALADLVHSHIQSNELCSKQLTLSCPLCVNPVCRETKSFFTSQQL opa1_mouse Dynamin-like 120 kDa protein, mitochondrial Optic atrophy protein 1 homolog Large GTP-binding protein Opa1 protein protein mouse Mus musculus Mouse MWRAGRAAVACEVCQSLVKHSSGIQRNVPLQKLHLVSRSIYRSHHPALKLQRPQLRTPFQQFSSLTHLSLHKLKLSPIKYGYQPRRNFWPARLAARLLKLRYIILGSAVGGGYTAKKTFDEWKDMIPDLSDYKWIVPDFIWEIDEYIDLEKIRKALPSSEDLASLAPDLDKITESLSLLKDFFTAGSPGETAFRATDHGSESDKHYRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQGRLKDTENAIENMIGPDWKKRWMYWKNRTQEQCVHNETKNELEKMLKVNDEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFIDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKVKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK mic60_mouse MICOS complex subunit Mic60 Mitofilin Immt Mic60 Mitochondrial inner membrane protein protein protein mouse Mus musculus Mouse MLRACQLSGVTVAAQSCLCGKFVLRPLRPCRRYSTSSSSGLTAGKIAGAGLLFVGGGIGGTILYAKWDSHFRESVEKTIPYSDKLFGMVLGSAPYTVPLPKKPVQSGPLKISSVSEVMKDSKLPVAQSQKTKGDTPASAASTGAAQIISAAGDTLSVPAPAVQHEDTIKTECPNTNEGKSTSETTEEAFSSSVRERPPEEVAARLAQQEKQEQVEMESLAKSLEDALNRTSSVTLQTITAQNAAVQAVKAHSNILKTAMDNSEIAGEKKSAQWRTVEGALKERRKAVDEAADALLKAKEELEKMKTIIEDAKKREIAGATPHITAAEGRLHNMIVDLDNVVKKVQAAQSEAKVVSQYHELVVQARDDFRKELDSITPDITPGWKGMSISDLAGKLSTDDLNSLIAHAHRRIDQLNRELAQQKATEKQHIELALEKHKLEEKRTFDSAVAKALEHHRSEIQAEQDRKVEEVRDAMENEMRTQLRRQAAAHTDHLRDVLKVQEQELKYEFEQGLSEKLSEQELEFRRRSQEQMDSFTLDINTAYARLRGIEQAVQSHAVAEEEARKAHQLWLSVEALKYSMKTSSAEMPTIPLGSAVEAIRVNCSDNEFTQALTAAIPPESLTRGVYSEETLRARFYAVQKLARRVAMIDETRNSLYQYFLSYLQSLLLFPPKQLKPPAELYPEDINTFKLLSYASYCIEHGDLELAAKFVNQLKGESRRVAQDWLKEARMTLETKQIVEILTAYASAVGIGTTQVQQE pgrc2_mouse Membrane-associated progesterone receptor component 2 Pgrmc2 protein protein mouse Mus musculus Mouse MAAGDGDVKLSTLGSGGESGGDGSPGGAGATAARSSWVAALLATGGEMLLNVALVALVLLGAYRLWVRWGRRGLCSGPGAGEESPAATLPRMKKRDFSLEQLRQYDGARTPRILLAVNGKVFDVTKGSKFYGPAGPYGIFAGRDASRGLATFCLDKDALRDEYDDLSDLNAVQMESVREWEMQFKEKYDYVGRLLKPGEEPSEYTDEEDTKDHSKQD pgrc1_mouse Membrane-associated progesterone receptor component 1 Pgrmc1 Pgrmc protein protein mouse Mus musculus Mouse MAAEDVVATGADPSELEGGGLLHEIFTSPLNLLLLGLCIFLLYKIVRGDQPGASGDNDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYDDLSDLTPAQQETLSDWDSQFTFKYHHVGKLLKEGEEPTVYSDDEEPKDETARKNE stea3_mouse Metalloreductase STEAP3 Dudulin-2 Protein nm1054 Six-transmembrane epithelial antigen of prostate 3 Tumor suppressor-activated pathway protein 6 Steap3 Tsap6 protein protein mouse Mus musculus Mouse MSGEMDKPLISRRLVDSDGSLAEVPKEAPKVGILGSGDFARSLATRLVGSGFSVVVGSRNPKRTAGLFPSLAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLADQLAGKILVDVSNPTEKEHLQHRQSNAEYLASLFPACTVVKAFNVISAWALQAGPRDGNRQVLICSDQPEAKRTISEMARAMGFTPLDMGSLASAREVEAIPLRLLPSWKVPTLLALGLFVCFYTYNFIRDVLQPYIRKDENKFYKMPLSVVNTTLPCVAYVLLSLVYLPGVLAAALQLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFFAMLHALYSFCLPLRRSHRYDLVNLAVKQVLANKSRLWVEEEVWRMEIYLSLGVLALGMLSLLAVTSLPSIANSLNWKEFSFVQSTLGFVALILSTMHTLTYGWTRAFEENHYKFYLPPTFTLTLLLPCVIILAKGLFLLPCLSRRLTKIRRGWEKDGAVKFMLPGDHTQGEKTSHV tfr1_mouse Transferrin receptor protein 1 Tfrc Trfr protein protein mouse Mus musculus Mouse MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAADEEENADNNMKASVRKPKRFNGRLCFAAIALVIFFLIGFMSGYLGYCKRVEQKEECVKLAETEETDKSETMETEDVPTSSRLYWADLKTLLSEKLNSIEFADTIKQLSQNTYTPREAGSQKDESLAYYIENQFHEFKFSKVWRDEHYVKIQVKSSIGQNMVTIVQSNGNLDPVESPEGYVAFSKPTEVSGKLVHANFGTKKDFEELSYSVNGSLVIVRAGEITFAEKVANAQSFNAIGVLIYMDKNKFPVVEADLALFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAAAEKLFGKMEGSCPARWNIDSSCKLELSQNQNVKLIVKNVLKERRILNIFGVIKGYEEPDRYVVVGAQRDALGAGVAAKSSVGTGLLLKLAQVFSDMISKDGFRPSRSIIFASWTAGDFGAVGATEWLEGYLSSLHLKAFTYINLDKVVLGTSNFKVSASPLLYTLMGKIMQDVKHPVDGKSLYRDSNWISKVEKLSFDNAAYPFLAYSGIPAVSFCFCEDADYPYLGTRLDTYEALTQKVPQLNQMVRTAAEVAGQLIIKLTHDVELNLDYEMYNSKLLSFMKDLNQFKTDIRDMGLSLQWLYSARGDYFRATSRLTTDFHNAEKTNRFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVENLKLRQKNITAFNETLFRNQLALATWTIQGVANALSGDIWNIDNEF m2om_mouse Mitochondrial 2-oxoglutarate/malate carrier protein Slc25a11 Solute carrier family 25 member 11 protein protein mouse Mus musculus Mouse MAATASPGAGRMDGKPRTSPKSVKFLFGGLAGMGATVFVQPLDLVKNRMQLSGEGAKTREYKTSFHALTSILKTEGLKGIYTGLSAGLLRQATYTTTRLGIYTVLFERLTGADGTPPGFLLKALIGMTAGATGAFVGTPAEVALIRMTADGRLPADQRRGYKNVFNALVRIAREEGVPTLWRGCIPTMARAVVVNAAQLASYSQSKQFLLDSGYFSDNILCHFCASMISGLVTTAASMPVDIVKTRIQNMRMIDGKPEYKNGLDVLLKVVRYEGFFSLWKGFTPYYARLGPHTVLTFIFLEQMNKAYKRLFLSG vdac2_mouse Voltage-dependent anion-selective channel protein 2 Voltage-dependent anion-selective channel protein 6 Outer mitochondrial membrane protein porin 2 Vdac2 Vdac6 protein protein mouse Mus musculus Mouse MAECCVPVCPRPMCIPPPYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTSGSSNTDTGKVSGTLETKYKWCEYGLTFTEKWNTDNTLGTEIAIEDQICQGLKLTFDTTFSPNTGKKSGKIKSAYKRECINLGCDVDFDFAGPAIHGSAVFGYEGWLAGYQMTFDSAKSKLTRSNFAVGYRTGDFQLHTNVNNGTEFGGSIYQKVCEDFDTSVNLAWTSGTNCTRFGIAAKYQLDPTASISAKVNNSSLIGVGYTQTLRPGVKLTLSALVDGKSFNAGGHKLGLALELEA adt2_mouse ADP/ATP translocase 2 Adenine nucleotide translocator 2 ADP,ATP carrier protein 2 Solute carrier family 25 member 5 Slc25a5 Ant2 Aac2 protein protein mouse Mus musculus Mouse MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFKGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIFISWMIAQSVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGSKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT adt1_mouse ADP/ATP translocase 1 Slc25a4 Anc1 Ant1 Adenine nucleotide translocator 1 ADP,ATP carrier protein 1 Solute carrier family 25 member 4 ADP,ATP carrier protein, heart/skeletal muscle isoform T1 Aac1 protein protein mouse Mus musculus Mouse MGDQALSFLKDFLAGGIAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGSSQREFNGLGDCLTKIFKSDGLKGLYQGFSVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIIVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTLDCWRKIAKDEGANAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV abcb7_mouse Iron-sulfur clusters transporter ABCB7, mitochondrial Abcb7 Abc7 ATP-binding cassette transporter 7 ATP-binding cassette sub-family B member 7, mitochondrial protein protein mouse Mus musculus Mouse MALLAIHSWRWAAAAVAFEKHKHSAVLTRALVSMCGSGPRWSSSQRGASGSARLSQTTESLRNTTQQRWGKDNSRQLLDATKALQTWPLIEKRTCWHGHAGGGLHTDPKEGLKDVDTRKIIKAMLSYVWPEDRPDLRARVAISLGFLGGAKAMNIVVPFMFKYAVDSLNQMSGNMLNLSDAPNTVATMATAVLIGYGVSRAGAAFFNEVRNAVFGKVAQNSIRRIAKNVFLHLHNLDLGFHLSRQTGALSKAIDRGTRGISFVLSALVFNLLPIVFEMMLVSSVLYYKCGAQFALVTLGTLGAYTAFTVAVTRWRTRFRIEMNKADNDAGNAAIDSLLNYETVKYFNNEKYEAQRYDGFLKTYETASLKSTSTLAMLNFGQNAIFSVGLTAIMVLASQGIVAGALTVGDLVMVNGLLFQLSLPLNFLGTVYRETRQALIDMNTLFTLLKVDTRIKDKVMAPPLQITPQTATVAFDNVHFEYIEGQKVLNGVSFEVPAGKKVAIVGGSGSGKSTIVRLLFRFYEPQKGSIYLAGQNLQDVSLESLRRAVGVVPQDAVLFHNTIYYNLLYGNINASPEEVYAVAKLAGLHDAILRMPHGYDTQVGERGLKLSGGEKQRVAIARAILKNPPVILYDEATSSLDSITEETILGAMRDVVKHRTSIFIAHRLSTVVDADEIIVLSQGKVAERGTHYGLLANSSSIYSEMWHTQSNRVQNQDSLGWDAKKESLSKEEERKKLQEEIVNSVKGCGNCSC abcba_mouse ATP-binding cassette sub-family B member 10, mitochondrial ABC-mitochondrial erythroid protein ATP-binding cassette transporter 10 Abcb10 protein protein mouse Mus musculus Mouse MRAPSARALLLIPRRGPAVRAWAPAVSSRIWLASEWTPLVRAWTSLIHKPGSGLRFPAPLSGLPGGVGQWATSSGARRCWVLAGPRAAHPLFARLQGAAATGVRDLGNDSQRRPAATGRSEVWKLLGLVRPERGRLSAAVGFLAVSSVITMSAPFFLGRIIDVIYTNPSEGYGDSLTRLCAVLTCVFLCGAAANGIRVYLMQSSGQSIVNRLRTSLFSSILRQEVAFFDKTRTGELINRLSSDTALLGRSVTENLSDGLRAGAQASVGVGMMFFVSPSLATFVLSVVPPISVLAVIYGRYLRKLSKATQDSLAEATQLAEERIGNIRTIRAFGKEMTEVEKYTGRVDQLLQLAQKEALARAGFFGAAGLSGNLIVLSVLYKGGLLMGSAHMTVGELSSFLMYAFWVGLSIGGLSSFYSELMKGLGAGGRLWELLERQPRLPFNEGMVLDEKTFQGALEFRNVHFTYPARPEVSVFQDFSLSIPSGSVTALVGPSGSGKSTVVSLLLRLYDPNSGTVSLDGHDIRQLNPVWLRSKIGTVSQEPVLFSCSVAENIAYGADNLSSVTAQQVERAAEVANAAEFIRSFPQGFDTVVGEKGILLSGGQKQRIAIARALLKNPKILLLDEATSALDAENEHLVQEALDRLMEGRTVLIIAHRLSTIKNANFVAVLDHGKICEHGTHEELLLKPNGLYRKLMNKQSFLSYNGAEQFLEPARA sucb1_mouse Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial ATP-specific succinyl-CoA synthetase subunit beta Succinyl-CoA synthetase beta-A chain Sucla2 protein protein mouse Mus musculus Mouse MAASMFYGRQLAAAALRSHRPQTTLRAAAQVLGNSGLFNKHGLQVQQQQQRTLSLHEYLSMELLQEAGVSVPKGFVAKSSDEAYAIAKKLGSKDVVIKAQVLAGGRGKGTFTSGLKGGVKIVFSPEEAKAVSSQMIGQKLITKQTGEKGRICNQVLVCERKYPRREYYFAITMERSFQGPVLIGSAQGGVNIEDVAAENPEAIVKEPIDIVEGIKKEQAVTLAQKMGFPSNIVDSAAENMIKLYNLFLKYDATMVEINPMVEDSDGKVLCMDAKINFDSNSAYRQKKIFDLQDWSQEDERDKEAANADINYIGLDGSIGCLVNGAGLAMATMDIIKLHGGTPANFLDVGGGATVQQVTEAFKLITSDKKVQAILVNIFGGIMRCDVIAQGIVMAVKDLEIRIPVVVRLQGTRVDDAKALIADSGLKILACDDLDEAAKMVVKLSEIVTLAKEAHVDVKFQLPI hem0_mouse 5-aminolevulinate synthase, erythroid-specific, mitochondrial 5-aminolevulinic acid synthase 2 Delta-ALA synthase 2 Delta-aminolevulinate synthase 2 Alas2 protein protein mouse Mus musculus Mouse MVAAAMLLRSCPVLSQGPTGLLGKVAKTYQFLFSIGRCPILATQGPTCSQIHLKATKAGGDSPSWAKSHCPFMLSELQDRKSKIVQRAAPEVQEDVKTFKTDLLSTMDSTTRSHSFPSFQEPEQTEGAVPHLIQNNMTGSQAFGYDQFFRDKIMEKKQDHTYRVFKTVNRWANAYPFAQHFSEASMASKDVSVWCSNDYLGISRHPRVLQAIEETLKNHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKLLPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPGHLKKLLEKSDPKTPKIVAFETVHSMDGAICPLEELCDVAHQYGALTFVDEVHAVGLYGARGAGIGERDGIMHKLDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGFPVIPCPSHIIPIRVGNAALNSKICDLLLSKHSIYVQAINYPTVPRGEELLRLAPSPHHSPQMMENFVEKLLLAWTEVGLPLQDVSVAACNFCHRPVHFELMSEWERSYFGNMGPQYVTTYA ppox_mouse Protoporphyrinogen oxidase Ppox protein protein mouse Mus musculus Mouse MGRTVIVLGGGISGLAASYHLIRGPSPPKVILVEGSKRLGGWIRSIRGSDGAIFELGPRGIRPAGALGARTLLLVSELGLESEVLPVRGDHPAAQNRFLYVGGTLHPLPSGLRGLLRPSPPFSKPLFWAGLRELLKPRGKEPDETVHSFAQRRLGPEVASLAMDSLCRGVFAGNSRELSIRSCFPSLFQAEQTHRSILLGLLLGAGQSPQPDSSLIRQARAERWSQWSLRGGLEVLPQALHNHLASKGVTVLSGQPVCGLSLQPEGRWKVSLGDSSLEADHIISAIPASELSKLLPAEAAPLARILSTIKAVSVAVVNLQYRGACLPVQGFGHLVPSSEDPTVLGIVYDSVAFPEQDGNPPSLRVTVMLGGYWLQKLKAAGHQLSPELFQQQAQEAAATQLGLKEPPSHCLVHLHKNCIPQYTIGHWQKLDSAMQFLTAQRLPLTLAGASYEGVAVNDCIESGRQAAVAVLGTESNS pgrc1_human Membrane-associated progesterone receptor component 1 Dap1 IZA PGRMC1 HPR6.6 PGRMC protein protein human Homo sapiens Human MAAEDVVATGADPSDLESGGLLHEIFTSPLNLLLLGLCIFLLYKIVRGDQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYDDLSDLTAAQQETLSDWESQFTFKYHHVGKLLKEGEEPTVYSDEEEPKDESARKND pgrc2_human Membrane-associated progesterone receptor component 2 Progesterone membrane-binding protein Steroid receptor protein DG6 PGRMC2 DG6 PMBP protein protein human Homo sapiens Human MAAGDGDVKLGTLGSGSESSNDGGSESPGDAGAAAEGGGWAAAALALLTGGGEMLLNVALVALVLLGAYRLWVRWGRRGLGAGAGAGEESPATSLPRMKKRDFSLEQLRQYDGSRNPRILLAVNGKVFDVTKGSKFYGPAGPYGIFAGRDASRGLATFCLDKDALRDEYDDLSDLNAVQMESVREWEMQFKEKYDYVGRLLKPGEEPSEYTDEEDTKDHNKQD mesoporphyrin ix mesoporphyrin IX small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) fech-2 2844455 N/A fech 2844460 unspecified role unspecified role prey prey N/A Apool 2844461 unspecified role unspecified role prey prey N/A FECH 2844462 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A opa1 2844463 unspecified role unspecified role prey prey N/A Immt Apool 2844464 unspecified role unspecified role prey prey N/A pgrmc2 2844465 unspecified role unspecified role prey prey N/A pgrmc1 2844466 unspecified role unspecified role prey prey association association false false false Table 1, fig 1 pgrmc1-1 2844455 N/A fech 2844460 unspecified role unspecified role prey prey N/A Metalloreductase steap3 (steap3) 2844467 unspecified role unspecified role prey prey N/A transferrin receptor protein 1 (tfrc) 2844468 unspecified role unspecified role prey prey N/A 2-oxoglutarate/malate carrier slc25a11 (ocg) 2844469 unspecified role unspecified role prey prey N/A voltage dependent anion selective protein channel 2 (vdac-2) 2844470 unspecified role unspecified role prey prey N/A ADP/ATP translocase 2 slc25a5 (ant2) 2844471 unspecified role unspecified role prey prey N/A ADP/ATP translocase 1 slc25a4 (ant1) 2844472 unspecified role unspecified role prey prey N/A abcb7 2844473 unspecified role unspecified role prey prey N/A abcb10 2844474 unspecified role unspecified role prey prey N/A sucla2 2844475 unspecified role unspecified role prey prey N/A alas-2 2844476 unspecified role unspecified role prey prey N/A ppox 2844477 unspecified role unspecified role prey prey N/A opa1 2844463 unspecified role unspecified role prey prey N/A Immt 2844464 unspecified role unspecified role prey prey N/A pgrmc2 2844465 unspecified role unspecified role prey prey N/A pgrmc1 2844466 unspecified role unspecified role prey prey N/A Apool 2844461 unspecified role unspecified role prey prey N/A PGRMC1 2844478 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Table 1, suppl. table 1, fig. 1 pgrmc1-abcb10-1 2844455 N/A fech 2844460 unspecified role unspecified role prey prey N/A Apool 2844461 unspecified role unspecified role prey prey N/A Metalloreductase steap3 (steap3) 2844467 unspecified role unspecified role prey prey N/A transferrin receptor protein 1 (tfrc) 2844468 unspecified role unspecified role prey prey N/A 2-oxoglutarate/malate carrier slc25a11 (ocg) 2844469 unspecified role unspecified role prey prey N/A voltage dependent anion selective protein channel 2 (vdac-2) 2844470 unspecified role unspecified role prey prey N/A ADP/ATP translocase 2 slc25a5 (ant2) 2844471 unspecified role unspecified role prey prey N/A ADP/ATP translocase 1 slc25a4 (ant1) 2844472 unspecified role unspecified role prey prey N/A abcb7 2844473 unspecified role unspecified role prey prey N/A abcb10 2844474 unspecified role unspecified role prey prey N/A sucla2 2844475 unspecified role unspecified role prey prey N/A alas-2 2844476 unspecified role unspecified role prey prey N/A opa1 2844463 unspecified role unspecified role prey prey N/A Immt 2844464 unspecified role unspecified role prey prey N/A pgrmc2 2844465 unspecified role unspecified role prey prey N/A pgrmc1 2844466 unspecified role unspecified role prey prey N/A PGRMC2 2844479 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Table 1, suppl. table 1, fig. 1 fech-fech-2 2844455 N/A fech 2844460 unspecified role unspecified role prey prey N/A FECH 2844462 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A pgrmc2 2844465 unspecified role unspecified role prey prey N/A pgrmc1 2844466 unspecified role unspecified role prey prey association association false false false Table S2 fech-fech-3 2844455 N/A fech 2844460 unspecified role unspecified role prey prey N/A PGRMC1 2844478 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A pgrmc1 2844466 unspecified role unspecified role prey prey association association false false false Table S2 pgrmc1-fech-1 2844455 N/A fech 2844460 unspecified role unspecified role prey prey N/A PGRMC2 2844479 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Table S2 fech-fech-1 2844456 N/A fech 2844460 unspecified role unspecified role prey prey N/A immt 2844464 unspecified role unspecified role prey prey N/A ppox 2844477 unspecified role unspecified role prey prey N/A sucla2 2844475 unspecified role unspecified role prey prey N/A FECH 2844462 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A abcb10 2844474 unspecified role unspecified role prey prey N/A abcb7 2844473 unspecified role unspecified role prey prey N/A pgrmc2 2844465 unspecified role unspecified role prey prey N/A pgrmc1 2844466 unspecified role unspecified role prey prey association association false false false 2A abcb10-2 2844456 N/A fech 2844460 unspecified role unspecified role prey prey N/A immt 2844464 unspecified role unspecified role prey prey N/A ppox 2844477 unspecified role unspecified role prey prey N/A sucla2 2844475 unspecified role unspecified role prey prey N/A abcb10 2844474 unspecified role unspecified role prey prey N/A abcb7 2844473 unspecified role unspecified role prey prey N/A pgrmc2 2844465 unspecified role unspecified role prey prey N/A pgrmc1 2844466 unspecified role unspecified role prey prey N/A PGRMC1 2844478 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false 2A fech-pgrmc1-3 2844457 N/A 2844480 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2844478 inhibitor inhibitor neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 2844462 enzyme enzyme neutral component neutral component purified purified P22830:p.Phe110Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 9 In the presence of PGRMC1 the activity of the F110A variant showed a level of inhibition slightly higher than that of the WT enzyme. fech-pgrmc1-4 2844457 N/A 2844480 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 2844462 enzyme enzyme neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P22830:p.Phe110Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 9i fech-pgrmc1-1 2844458 N/A 2844478 unspecified role unspecified role prey prey N/A 2844462 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 2B fech-pgrmc1-2 2844459 N/A PGRMC1 2844478 unspecified role unspecified role prey prey cell lysate cell lysate ecoli Escherichia coli (strain K12) N/A FECH 2844462 unspecified role unspecified role bait bait cell lysate cell lysate n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P22830:p.Phe110Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P22830:p.Glu343Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A,3B