UniProt UniProt http://www.uniprot.org bahl-2016-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bahl K., Xie S., Spagnol G., Sorgen P., Naslavsky N., Caplan S. Species origin of used constructs according sequence fragments and description of mutants given in the publication (eg fig 5A, 7A), and publications from the group, such as ref 22 (PMID 19864458) and PMIDs 12032069 and 15020713) scaplan@unmc.edu The Journal of biological chemistry 2016 imex curation Only protein-protein interactions bahl-2016-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bahl K., Xie S., Spagnol G., Sorgen P., Naslavsky N., Caplan S. Species origin of used constructs according sequence fragments and description of mutants given in the publication (eg fig 5A, 7A), and publications from the group, such as ref 22 (PMID 19864458) and PMIDs 12032069 and 15020713) scaplan@unmc.edu The Journal of biological chemistry 2016 imex curation Only protein-protein interactions bahl-2016-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Bahl K., Xie S., Spagnol G., Sorgen P., Naslavsky N., Caplan S. Species origin of used constructs according sequence fragments and description of mutants given in the publication (eg fig 5A, 7A), and publications from the group, such as ref 22 (PMID 19864458) and PMIDs 12032069 and 15020713) scaplan@unmc.edu The Journal of biological chemistry 2016 imex curation Only protein-protein interactions milk1_human MICAL-like protein 1 MICALL1 KIAA1668 MIRAB13 Molecule interacting with Rab13 protein protein human Homo sapiens Human MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEGTFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPAPRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPSPALSVESLSSESASQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGSKPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS ehd1_human EH domain-containing protein 1 Testilin CDABP0131 EHD1 PAST PAST1 PAST homolog 1 protein protein human Homo sapiens Human MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE ehd3_human EH domain-containing protein 3 EHD3 EHD2 PAST3 PAST homolog 3 protein protein human Homo sapiens Human MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE anfy1_human Rabankyrin-5 Ankyrin repeat and FYVE domain-containing protein 1 ANKFY1 ANKHZN KIAA1255 Ankyrin repeats hooked to a zinc finger motif protein protein human Homo sapiens Human MAEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKESSSESFISRLLAIVADLYEQEQYSDLKIKVGDRHISAHKFVLAARSDSWSLANLSSTKELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLVSHKADVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYSSKKHSADVMSEMAQIAEALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVNGTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEAASLTSLADSVHLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGAAINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGGCLQTLLHRAIDENNEPTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPIHVAISSQHGVIIQLLVSHPDIHLNVRDRQGLTPFACAMTFKNNKSAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVHANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS micall1-ehd1-1 2844599 N/A 2844602 unspecified role unspecified role bait bait Q8N3F8:p.Glu429_Asp433delinsAlaAlaAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8N3F8:p.Glu428_Asp433delinsAlaAlaAlaAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false MICALL1 mutants with wt positions 425-433 NPFEEEED replaced with NPFEEEEA or NPFEEEEAA or NPFEEEAAA or NPFEEAAAA also interact N/A 2844603 unspecified role unspecified role prey prey EHD1 mutant replacing wt positions 519-520 AD with NE also interacts physical association physical association false false false 5C and E, 7C micall1-ehd3-1 2844599 N/A 2844602 unspecified role unspecified role bait bait Q8N3F8:p.Glu428_Asp433delinsAlaAlaAlaAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false MICALL1 mutants with wt positions 425-433 NPFEEEED replaced with NPFEEEEA or NPFEEEEAA or NPFEEEAAA or NPFEEAAAA or NPFEAAAAA also interact N/A 2844604 unspecified role unspecified role prey prey EHD3 mutants NE519-520AD or AHLL523-526PHLV or D459N or M447T or A437V or KVAE532-535RHE or N519A or E520D also interact physical association physical association false false false 5C and E ankfy1-ehd1-1 2844599 N/A 2844605 unspecified role unspecified role bait bait ANKFY1/rabankyrin-5 mutants having wt positions 421-426 NPFEDV replaced with NPFEDE or NPFEDEE or NPFEDEEE also interact N/A 2844603 unspecified role unspecified role prey prey EHD1 construct ala_asp519-520asn_glu also interacts physical association physical association false false false 5C and E, 7B ankfy1-ehd3-2 2844599 N/A 2844605 unspecified role unspecified role bait bait N/A 2844604 unspecified role unspecified role prey prey Q9NZN3:p.Asn519_Glu520delinsAlaAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false Q9NZN3:p.Glu520Asp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false Q9NZN3:p.Asn519Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false EHD3 constructs asn_glu519-520ala_asp, ala_his_leu_leu523-526pro_his_leu_val, asp-429asn, met477thr, ala473val, lys-val_ala_glu532-535arg_his_glu, asn519ala and glu520asp also interact physical association physical association false false false 5 E ehd1-micall1-2 2844599 N/A 2844602 unspecified role unspecified role prey prey N/A 2844603 unspecified role unspecified role bait bait physical association physical association false false false 8B ehd3-micall1-2 2844599 N/A 2844604 unspecified role unspecified role bait bait N/A 2844602 unspecified role unspecified role prey prey physical association physical association false false false 8B ehd3-ankfy1-3 2844599 N/A 2844605 unspecified role unspecified role prey prey Q9P2R3:p.Gln418_Val420delinsLysProTyr mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false Q9P2R3:p.Val420Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false Q9P2R3:p.Gln418Lys mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false Q9P2R3:p.Val420Tyr mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false N/A 2844604 unspecified role unspecified role bait bait physical association physical association false false false 8 B and C, 9A ehd1-ankfy1-3 2844599 N/A 2844603 unspecified role unspecified role bait bait N/A 2844605 unspecified role unspecified role prey prey Q9P2R3:p.Ser419Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ANKFY1/Rabankyrin-5 mutants wt pos 418-420 QSV changed to KPY or KSV or QSY or QSA also interact physical association physical association false false false 8B and 9A ehd1-micall1-1 2844600 N/A 2844602 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2844603 unspecified role unspecified role neutral component neutral component eh domain sufficient to bind sufficient binding region sufficient to bind range range range range false 2844645 2844643 direct interaction direct interaction false false false 2844600 4 ehd3-micall1-1 2844600 N/A 2844602 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2844604 unspecified role unspecified role neutral component neutral component eh domain sufficient to bind sufficient binding region sufficient to bind range range range range false 2844650 2844648 direct interaction direct interaction false false false 2844600 4 ehd1-ankfy1-2 2844601 N/A 2844603 unspecified role unspecified role bait bait eh domain sufficient to bind sufficient binding region sufficient to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 2844605 unspecified role unspecified role prey prey cell lysate cell lysate region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false 5D ehd3-ankfy1-1 2844601 N/A 2844604 unspecified role unspecified role bait bait eh domain sufficient to bind sufficient binding region sufficient to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9NZN3:p.Asn519_Glu520delinsAlaAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2844605 unspecified role unspecified role prey prey cell lysate cell lysate region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false 5C