MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint fliegmann-2016-1 in vitro In vitro protein kinase assay protein kinase assay ms participant Identification by mass spectrometry ms participant Fliegmann J., Jauneau A., Pichereaux C., Rosenberg C., Gasciolli V., Timmers AC., Burlet-Schiltz O., Cullimore J., Bono JJ. judith.fliegmann@sfr.fr,jean-jacques.bono@toulouse.inra.fr judith.fliegmann@sfr.fr; jean-jacques.bono@toulouse.inra.fr FEBS letters 2016 Only protein-protein interactions imex curation imex curation fliegmann-2016-2 yeast Saccharomyces cerevisiae Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Fliegmann J., Jauneau A., Pichereaux C., Rosenberg C., Gasciolli V., Timmers AC., Burlet-Schiltz O., Cullimore J., Bono JJ. judith.fliegmann@sfr.fr,jean-jacques.bono@toulouse.inra.fr judith.fliegmann@sfr.fr; jean-jacques.bono@toulouse.inra.fr FEBS letters 2016 Only protein-protein interactions imex curation imex curation fliegmann-2016-4 nicbe Nicotiana benthamiana fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag visualisation tag visualisation Fliegmann J., Jauneau A., Pichereaux C., Rosenberg C., Gasciolli V., Timmers AC., Burlet-Schiltz O., Cullimore J., Bono JJ. when LYR3/GFP and LYK3/mCherry were expressed together, addition of LCO at 10 8 M significantly reduced the FRET efficiency to 2.6%, in comparison to 13% without LCO addition (Table 2, Fig. S8). These results indicate a negative effect of LCO binding to MtLYR3 either on its ability to interact with MtLYK3 or on its conformation in the protein complex. judith.fliegmann@sfr.fr,jean-jacques.bono@toulouse.inra.fr judith.fliegmann@sfr.fr; jean-jacques.bono@toulouse.inra.fr FEBS letters 2016 Only protein-protein interactions imex curation imex curation fliegmann-2016-3 nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fliegmann J., Jauneau A., Pichereaux C., Rosenberg C., Gasciolli V., Timmers AC., Burlet-Schiltz O., Cullimore J., Bono JJ. judith.fliegmann@sfr.fr,jean-jacques.bono@toulouse.inra.fr judith.fliegmann@sfr.fr; jean-jacques.bono@toulouse.inra.fr FEBS letters 2016 Only protein-protein interactions imex curation imex curation lyk3_medtr LysM domain receptor-like kinase 3 LYK3 HCL RLK3 MTR_5g086130 LysM receptor kinase K1B Protein HAIR CURLING protein protein medtr medtr Barrel medic Medicago tribuloides MNLKNGLLLFILFLDCVFFKVESKCVKGCDVALASYYIIPSIQLRNISNFMQSKIVLTNSFDVIMSYNRDVVFDKSGLISYTRINVPFPCECIGGEFLGHVFEYTTKEGDDYDLIANTYYASLTTVELLKKFNSYDPNHIPVKAKINVTVICSCGNSQISKDYGLFVTYPLRSDDTLAKIATKAGLDEGLIQNFNQDANFSIGSGIVFIPGRDQNGHFFPLYSRTGIAKGSAVGIAMAGIFGLLLFVIYIYAKYFQKKEEEKTKLPQTSRAFSTQDASGSAEYETSGSSGHATGSAAGLTGIMVAKSTEFTYQELAKATNNFSLDNKIGQGGFGAVYYAELRGEKTAIKKMDVQASSEFLCELKVLTHVHHLNLVRLIGYCVEGSLFLVYEHIDNGNLGQYLHGIGTEPLPWSSRVQIALDSARGLEYIHEHTVPVYIHRDVKSANILIDKNLRGKVADFGLTKLIEVGNSTLHTRLVGTFGYMPPEYAQYGDVSPKIDVYAFGVVLYELITAKNAVLKTGESVAESKGLVQLFEEALHRMDPLEGLRKLVDPRLKENYPIDSVLKMAQLGRACTRDNPLLRPSMRSIVVALMTLSSPTEDCDDDSSYENQSLINLLSTR g7kat5_medtr MtrunA17_Chr5g0403381 MTR_5g019050 11409451 protein protein medtr medtr Barrel medic Medicago tribuloides MHLFPFIPFFIFNLFFFFFVSNISIILGQQPYIGLATNACPRKGDSKSIRGYTCNGKTQTCQAYLTFRTQPIYSSVSTISSLLGSNPSQLAEINSVSLNETFETNKMVIVPVNCSCSGNYYQANTSYVFQNTDTYFIVANNTFEGLSTCQALMHENHNPGDVYPGRKLLVPLRCACPTKNQTQKNIKYLLSYLVDWGDSVSFISDKFGVNFRTTLEANTLSLTQSTIYPFTTLLVPLFDKPSSSQIQTHHSPSSSPPLSSSSSTDKKSKKTWVYVVVGVLGGVVIVALILFLYAFISFKKGKKKNDFLVSVSESTIFEEKEKPMKKEDEKLSEIICGIAQSFKVYDFEEIKVATDNFSPSCRVKGTVYRGLIKGDLAAIKKTEGDVSKEIQILNKVNHSNVIRLSGVSFNQGHWYLVYEYAANGALSDWLFSNKKMDDGNILSWIRRIKIALDVAIGVEYLHSFTSPPHIHKDLKCSNILLDSDFKAKVANLRHVRCVEEVENDEEFVATRHIVGTRGYMAPEYLENGLVSTKLDVYAFGILMLEIITGKEVGFMISKDNENLLDVLSGILGEKSGDEKLKEFMDPSLQGNYPFELAMFVIEIIQNCLNKDPGNRPAMDEIVPVLSRTLNSSLSWEM 1-93 2857724 N/A 2857728 enzyme enzyme neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2857729 enzyme target enzyme target substrate substrate neutral component neutral component purified purified phoser297 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false phoser314 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false phothr315 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false phoser399 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false phothr628 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f1 f2 st1 st2 lyk3-1 2857724 N/A 2857728 self self neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false phosphorylation phosphorylation reaction phosphorylation false false false f1 g7kat5_medtr-lyk3-2 2857725 N/A 2857728 unspecified role unspecified role prey prey living cell living cell tag ubiquitin reconstruction tag ubiquitin reconstruction tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2857729 unspecified role unspecified role bait bait living cell living cell tag ubiquitin reconstruction tag ubiquitin reconstruction tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f3 g7kat5_medtr-lyk3-1 2857725 N/A 2857728 unspecified role unspecified role bait bait living cell living cell tag ubiquitin reconstruction tag ubiquitin reconstruction tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2857729 unspecified role unspecified role prey prey living cell living cell tag ubiquitin reconstruction tag ubiquitin reconstruction tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f3 1-95 2857726 N/A 2857728 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2857729 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell rfp tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false t1 sf6 sf7 1-96 2857726 N/A 2857728 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell rfp tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2857729 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false t1 sf6 sf7 1-94 2857727 N/A 2857728 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q6UD73:p.Gly343Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false N/A 2857729 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f4