HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html li-2015-20 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Porcine primary macrophage cDNA library Journal of virology 2015 imex curation Only protein-protein interactions imex curation li-2015-21 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2015 imex curation Only protein-protein interactions imex curation li-2015-22 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2015 imex curation Only protein-protein interactions imex curation li-2015-23 pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2015 imex curation Only protein-protein interactions imex curation li-2015-24 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2015 imex curation Only protein-protein interactions imex curation li-2015-25 pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 confocal microscopy confocal microscopy immunostaining immunostaining Li S., Wang J., He WR., Feng S., Li Y., Wang X., Liao Y., Qin HY., Li LF., Dong H., Sun Y., Luo Y., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Journal of virology 2015 imex curation Only protein-protein interactions imex curation q5u8x5_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus Classical swine fever virus MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLNNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVITIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTIIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSYEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYALSSTNEIGLLGAGGLTTTWEEYSHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKGALLTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRREKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGQVKQCKWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISAEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWKGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSSKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIRNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALKDSKEDIMKYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYKVTKYYDDNLSEIKPVIKMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYNGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIRPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKDITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNDWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMTEVPVICADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV a3ex84_pig Galectin LGALS3 protein protein pig Sus scrofa (Pig) Pig MADGFSLNDALSGSGKPNPQGWPGAWGNQPAGPGGYPGASYPGTYPGQGPPGAYPGQAPPGAYPGQAPPGAYPGPTAPGYPGPAAPGAYPGQPGGPGAYPPPAQPSAPGAYPATGPYGAPSGPLNVPYDLPLPGGVMPRMLITILGTVKPNANRLALDFKKGNDVAFHFNPRFNEDNRRVIVCNSKLDNNWGREERQMVFPFECGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRMRNLREISKLGISGDITLTSASHTMI k7zla6_pig IGHG3 protein protein pig Sus scrofa (Pig) Pig YNTAPSVYPLAPCGRDVSDHNVALGCLVSSYFPEPVTVTWNSGALSRVVHTFPSVLQPSGLYSLSSMVIVAASSLSTLSYTCNVYHPATNTKVDKRVDIEPPTPICPEICSCPAAEVLGAPSVFLFPPKPKDILMISRTPKVTCVVVDVSQEEAEVQFSWYVDGVQLYTAQTRPMEEQFNSTYRVVSVLPIQHQDWLKGKEFKCKVNNKDLLSPITRTISKATGPSRVPQVYTLPPAWEELSKSKVSITCLVTGFYPPDIDVEWQSNGQQEPEGNYRTTPPQQDVDGTYFLYSKLAVDKVRWQRGDLFQCAVMHEALHNHYTQKSVSQTPGK rack1_pig Receptor of activated protein C kinase 1 RACK1 GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Receptor for activated C kinase Receptor of activated protein kinase C 1 protein protein pig Sus scrofa (Pig) Pig MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR f1s3w8_pig CYP4B1 protein protein pig Sus scrofa (Pig) Pig MVPALLSLSLSHLSLWALGLILALGVLKLTSLLLRRQMLARAMDKFPGPPTHWIFGHALEIQQTGSLDKMVSWAHQFPHAHPLWLGPFLGFLNIYEPEYAKAVYSRGGEGSRERWDLLRAEGKGLLVLHGPKWFQHRKLLTPGFHHDVLKPYVAVMANSARVMLDKWEEKAREDKSFDIYHDVGHMALELLMKCTFGKGTSGLNYSDNAYHLAVRDLTLLMQQRLSSFQYHNDFIYWLTPHGRRFLRACRVAHDHTDQVIRERKAALQDKEEQERIQSRRHLDFLDILLGAQDEDRVKLSDADLRAEVDTFMFGGHDTTTRGISWFLYCMALNPEHQHRCREEIREILGDRDSIQGEDLGKMTYLTMCIRESFRLYPPVPQVYRQLSEPVSFVDGRSLPAGSLISLHIYALHRNSAVWPDPEVFDPLRFSLENMAGRHPFAFLPFSAGPRNCIGQRLP qki_pig Protein quaking QKI protein protein pig Sus scrofa (Pig) Pig MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN i3lii3_pig EEF2 protein protein pig Sus scrofa (Pig) Pig MAKGKESKTLVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPDGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL f1skj2_pig TXN2 protein protein pig Sus scrofa (Pig) Pig MVRSGIAAEAPLQARSHAGRWPSGQKPWMAQRLLLRRFLASVVSRKPSHSRWAPLASRALQTPQLSLSNLTGTPSQARSLYTTQVCTTTFNIQDGPDFQDRVVNSETPVVVDFHAQWCGPCKILGPRLEKVVAKQHGKVVMAKVDIDDHTDLAIEYEVSAVPTVLAIKNGDVVDKFVGIKDEDQLEAFLKKLIG igll5_pig Immunoglobulin lambda-like polypeptide 5 protein protein pig Sus scrofa (Pig) Pig similar to B9A064 q5u8x5_9flav-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865860 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-ighg3-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865861 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-rack1-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865862 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-cyp4b1-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865863 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-qki-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865864 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-eef2-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865865 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-txn-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865866 unspecified role unspecified role prey prey physical association physical association false false false table 2 q5u8x5_9flav-igll5_pig-1 2865853 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865867 unspecified role unspecified role prey prey physical association physical association false false false table 2 e2-trx2-1 2865854 N/A 2865859 unspecified role unspecified role bait bait c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q5U8X5:p.Asn705Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2865866 unspecified role unspecified role prey prey gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false 3C A chimeric E2 mutants between Shimen strain (UniProt id Q5U8X5) and C-strain (UniProt id O92364) is used in the study txn-q5u8x5_9flav-1 2865854 N/A 2865859 unspecified role unspecified role prey prey c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 2865866 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 1B, 2 e2-trx2-2 2865854 N/A 2865859 unspecified role unspecified role bait bait e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 2865866 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false 1C q5u8x5_9flav-txn2-4 2865854 N/A 2865859 unspecified role unspecified role prey prey c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 2865866 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 3C e2-txn2-1 2865855 N/A 2865859 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865866 unspecified role unspecified role neutral component neutral component mcherry mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 1D q5u8x5_9flav-txn2-1 2865856 N/A 2865859 unspecified role unspecified role neutral component neutral component c terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865866 unspecified role unspecified role neutral component neutral component myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false 1E q5u8x5_9flav-txn2-2 2865857 N/A 2865859 unspecified role unspecified role bait bait E2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865866 unspecified role unspecified role prey prey physical association physical association false false false Fig 1A q5u8x5_9flav-txn2-3 2865858 N/A 2865859 unspecified role unspecified role neutral component neutral component e2 region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 2865866 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1D