IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lin-2014-4 human-hematopoietic-progenitor Human-peripheral blood CD34+ hematopoieitc progenitor Human hematopoietic_progenitor_cells Peripheral blood CD34+ hematopoietic progenitor cells hemopoietic hemopoietic anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI., Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI. Source of endothelial cells in Fig 6 suppl. 1: EA.hy926 as stated by the corresponding author. There was only one NOTCH1 construct used to transfect cells and it was of human origin, as verified by the corresponding author, Dr Leonard Zon. zon@enders.tch.harvard.edu zon@ enders.tch.harvard.edu eLife 2014 Only protein-protein interactions imex curation imex curation lin-2014-6 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI., Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI. Source of endothelial cells in Fig 6 suppl. 1: EA.hy926 as stated by the corresponding author. There was only one NOTCH1 construct used to transfect cells and it was of human origin, as verified by the corresponding author, Dr Leonard Zon. zon@enders.tch.harvard.edu zon@ enders.tch.harvard.edu eLife 2014 Only protein-protein interactions imex curation imex curation lin-2014-7 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI., Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI. Source of endothelial cells in Fig 6 suppl. 1: EA.hy926 as stated by the corresponding author. There was only one NOTCH1 construct used to transfect cells and it was of human origin, as verified by the corresponding author, Dr Leonard Zon. zon@enders.tch.harvard.edu zon@ enders.tch.harvard.edu eLife 2014 Only protein-protein interactions imex curation imex curation lin-2014-5 human-ea.hy926 Homo sapiens EA.hy926 ea.hy926 EA.hy926 http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CRL-2922&Template=cellBiology This cell line was established by fusing primary human umbilical vein cells (HUVEC) with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI., Lin MI., Price EN., Boatman S., Hagedorn EJ., Trompouki E., Satishchandran S., Carspecken CW., Uong A., DiBiase A., Yang S., Canver MC., Dahlberg A., Lu Z., Zhang CC., Orkin SH., Bernstein ID., Aster JC., White RM., Zon LI. Source of endothelial cells in Fig 6 suppl. 1: EA.hy926 as stated by the corresponding author. There was only one NOTCH1 construct used to transfect cells and it was of human origin, as verified by the corresponding author, Dr Leonard Zon. zon@enders.tch.harvard.edu zon@ enders.tch.harvard.edu eLife 2014 Only protein-protein interactions imex curation imex curation lirb2_human Leukocyte immunoglobulin-like receptor subfamily B member 2 LILRB2 ILT4 LIR2 MIR10 CD85 antigen-like family member D Immunoglobulin-like transcript 4 Monocyte/macrophage immunoglobulin-like receptor 10 protein protein human Homo sapiens Human MTPIVTVLICLGLSLGPRTHVQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVVLLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKDTQPEDGVEMDTRAAASEAPQDVTYAQLHSLTLRRKATEPPPSQEREPPAEPSIYATLAIH notc1_human Neurogenic locus notch homolog protein 1 Translocation-associated notch protein TAN-1 NOTCH1 TAN1 protein protein human Homo sapiens Human MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK notch1-5 1902774 N/A 1902778 unspecified role unspecified role prey prey N/A 1902779 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6B notch1-6 1902774 N/A 1902778 unspecified role unspecified role bait bait N/A 1902779 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6C ANGPTL2 notch1-3 1902775 N/A 1902778 unspecified role unspecified role prey prey N/A 1902779 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6A top blot ANGPTL2 notch1-4 1902775 N/A 1902778 unspecified role unspecified role bait bait N/A 1902779 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6A middle blot notch1-7 1902776 N/A 1902778 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1902779 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6D, Fig. 6 Suppl 2 (double, pannel) notch1-8 1902776 N/A 1902778 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1902779 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6D and Fig. 6 Suppl 2 (double, pannel) notch1-2 1902777 N/A 1902779 unspecified role unspecified role bait bait N/A 1902778 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6_Supplement 1B ANGPTL2 notch1-1 1902777 N/A 1902778 unspecified role unspecified role bait bait N/A 1902779 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6_Supplement 1A ANGPTL2