IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gordiyenko-2013-1 yeast Saccharomyces cerevisiae Baker's yeast ms of complexes mass spectrometry studies of complexes ms of complexes sequence tag sequence tag identification sequence tag Gordiyenko Y., Schmidt C., Jennings MD., Matak-Vinkovic D., Pavitt GD., Robinson CV. graham.pavitt@manchester.ac.uk, carol.robinson@chem.ox.ac.uk Nature communications 2013 Only protein-protein interactions imex curation imex curation gordiyenko-2013-3 yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Gordiyenko Y., Schmidt C., Jennings MD., Matak-Vinkovic D., Pavitt GD., Robinson CV. graham.pavitt@manchester.ac.uk, carol.robinson@chem.ox.ac.uk Nature communications 2013 Only protein-protein interactions imex curation imex curation gordiyenko-2013-4 yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Gordiyenko Y., Schmidt C., Jennings MD., Matak-Vinkovic D., Pavitt GD., Robinson CV. Cross linking agent: BS3 (bis(sulfosuccinimidyl)suberate). Some cross-links are recorded as being observed in sub-complexes but all results have been concatenated into a single entry. Only inter- not intramolecular crosslinks have been captured. Ambiguous mappings were not captured. graham.pavitt@manchester.ac.uk, carol.robinson@chem.ox.ac.uk Nature communications 2013 Only protein-protein interactions imex curation imex curation gordiyenko-2013-2 yeast Saccharomyces cerevisiae Baker's yeast pull down pull down sequence tag sequence tag identification sequence tag Gordiyenko Y., Schmidt C., Jennings MD., Matak-Vinkovic D., Pavitt GD., Robinson CV. graham.pavitt@manchester.ac.uk, carol.robinson@chem.ox.ac.uk Nature communications 2013 Only protein-protein interactions imex curation imex curation if2b_yeast Eukaryotic translation initiation factor 2 subunit beta YPL237W SUI3 TIF212 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSDLAAELGFDPALKKKKKTKKVIPDDFDAAVNGKENGSGDDLFAGLKKKKKKSKSVSADAEAEKEPTDDIAEALGELSLKKKKKKTKDSSVDAFEKELAKAGLDNVDAESKEGTPSANSSIQQEVGLPYSELLSRFFNILRTNNPELAGDRSGPKFRIPPPVCLRDGKKTIFSNIQDIAEKLHRSPEHLIQYLFAELGTSGSVDGQKRLVIKGKFQSKQMENVLRRYILEYVTCKTCKSINTELKREQSNRLFFMVCKSCGSTRSVSSIKTGFQATVGKRRRM 34DE9323876CEE95 if2a_yeast Eukaryotic translation initiation factor 2 subunit alpha SUI2 TIF211 YJR007W J1429 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTSHCRFYENKYPEIDDIVMVNVQQIAEMGAYVKLLEYDNIEGMILLSELSRRRIRSIQKLIRVGKNDVAVVLRVDKEKGYIDLSKRRVSSEDIIKCEEKYQKSKTVHSILRYCAEKFQIPLEELYKTIAWPLSRKFGHAYEAFKLSIIDETVWEGIEPPSKDVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAAPLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATEDAELQALLESKELDNRSDSEDDEDESDDE AF4F1C80303A4E98 if2g_yeast Eukaryotic translation initiation factor 2 subunit gamma YER025W GCD11 TIF213 SUI4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLQDQEPSIIINGNLEPVGEPDIVEETEVVAQETQETQDADKPKKKVAFTGLEEDGETEEEKRKREFEEGGGLPEQPLNPDFSKLNPLSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDCYRSFKSDKEISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMREESALEHQKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAEIEDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKHWRLIGWATIKKGTTLEPIA D498AE62BC3E81CD ei2bg_yeast Translation initiation factor eIF-2B subunit gamma GCD1 eIF-2B GDP-GTP exchange factor subunit gamma GCD complex subunit GCD1 Guanine nucleotide exchange factor subunit GCD1 TIF223 TRA3 YOR260W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIQAFVFCGKGSNLAPFTQPDFPFQTQNKDSTAATSGDKLNELVNSALDSTVINEFMQHSTRLPKALLPIGNRPMIEYVLDWCDQADFKEISVVAPVDEIELIESGLTSFLSLRKQQFELIYKALSNSNHSHHLQDPKKINFIPSKANSTGESLQKELLPRINGDFVILPCDFVTDIPPQVLVDQFRNRDDNNLAMTIYYKNSLDSSIDKKQQQKQKQQQFFTVYSENEDSERQPILLDVYSQRDVTKTKYLQIRSHLLWNYPNLTVSTKLLNSFIYFCSFELCQLLKLGPQSMSRQASFKDPFTGNQQQQNPPTTDDDEDRNHDDDDDYKPSATSIQPTYFKKKNDLILDPINCNKSLSKVFRDLSRRSWQHSKPREPIGIFILPNETLFIRANNLNAYMDANRFVLKIKSQTMFTKNIQIQSAAIGADAIVDPKCQISAHSNVKMSVLGTQANIGSRCRVAGSLLFPGVHLGDEVILENCIIGPMAKIGSKCKLSNCYIEGHYVVEPKNNFKGETLANVYLDEDEEDELIYDDSVIAGESEIAEETDSDDRSDEDSDDSEYTDEYEYEDDGLFER EC25BAE00F4D1E94 ei2bb_yeast Translation initiation factor eIF-2B subunit beta GCD7 eIF-2B GDP-GTP exchange factor subunit beta GCD complex subunit GCD7 Guanine nucleotide exchange factor subunit GCD7 TIF222 L8003.17 YLR291C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSQAFTSVHPNAATSDVNVTIDTFVAKLKRRQVQGSYAIALETLQLLMRFISAARWNHVNDLIEQIRDLGNSLEKAHPTAFSCGNVIRRILAVLRDEVEEDTMSTTVTSTSVAEPLISSMFNLLQKPEQPHQNRKNSSGSSSMKTKTDYRQVAIQGIKDLIDEIKNIDEGIQQIAIDLIHDHEILLTPTPDSKTVLKFLITARERSNRTFTVLVTEGFPNNTKNAHEFAKKLAQHNIETLVVPDSAVFALMSRVGKVIIGTKAVFVNGGTISSNSGVSSVCECAREFRTPVFAVAGLYKLSPLYPFDVEKFVEFGGSQRILPRMDPRKRLDTVNQITDYVPPENIDIYITNVGGFNPSFIYRIAWDNYKQIDVHLDKNKA A5409FAF1594854C ei2bd_yeast Translation initiation factor eIF-2B subunit delta GCD2 eIF-2B GDP-GTP exchange factor subunit delta GCD complex subunit GCD2 Guanine nucleotide exchange factor subunit GCD2 TIF224 YGR083C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSESEAKSRSATPPSKAKQATPTTTAAANGEKKLTNKELKELKKQEKAAKRAAMKQANGISIEQQQQQAQMKKEKKQLQREQQQKREQKQKNANKKKQNERNVKKSTLFGHLETTEERRATILALTSAVSSPKTSRITAAGLMVPVVASALSGSNVLTASSLMPVGPNASSTVSASAPASTTTTLPASSAALSAGTSSASTNTPTAIQQEIASSNASDVAKTLASISLEAGEFNVIPGISSVIPTVLEQSFDNSSLISSVKELLLNKDLIHPSILLLTSHLAHYKIVGSIPRCIAMLEVFQIVIKDYQTPKGTTLSRNLTSYLSHQIDLLKKARPLSVTMGNAIRWLKQEISLIDPSTPDKAAKKDLCEKIGQFAKEKIELADQLIIDNASTQIEESTTIVTYGSSKVLTELLLHNAISLKKNIKVIVVDSRPLFEGRKMAETLRNAGVNVMYALITSLDTIFNMDVDYVFLGAHSILSNGFLYSRAGTAMLAMSAKRRNIPVLVCCESLKFSQRVQLDSVTFNELADPNDLVNIDYENPVERRGNKGALLNQFIKERKFEKKKLAMENKPKGNKIGGKKGSEGESKDASNEEDSNSKNILDGWQELPSLNIVNILYDLTPPEYIKKVITEFGALPPSSVPVILREYKGSA B188457543CE1AAE ei2be_yeast Translation initiation factor eIF-2B subunit epsilon GCD6 eIF-2B GDP-GTP exchange factor subunit epsilon GCD complex subunit GCD6 Guanine nucleotide exchange factor subunit GCD6 TIF225 YD8142.12 YD8142B.03 YDR211W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGKKGQKKSGLGNHGKNSDMDVEDRLQAVVLTDSYETRFMPLTAVKPRCLLPLANVPLIEYTLEFLAKAGVHEVFLICSSHANQINDYIENSKWNLPWSPFKITTIMSPEARCTGDVMRDLDNRGIITGDFILVSGDVLTNIDFSKMLEFHKKMHLQDKDHISTMCLSKASTYPKTRTIEPAAFVLDKSTSRCIYYQDLPLPSSREKTSIQIDPELLDNVDEFVIRNDLIDCRIDICTSHVPLIFQENFDYQSLRTDFVKGVISSDILGKHIYAYLTDEYAVRVESWQTYDTISQDFLGRWCYPLVLDSNIQDDQTYSYESRHIYKEKDVVLAQSCKIGKCTAIGSGTKIGEGTKIENSVIGRNCQIGENIRIKNSFIWDDCIIGNNSIIDHSLIASNATLGSNVRLNDGCIIGFNVKIDDNMDLDRNTKISASPLKNAGSRMYDNESNEQFDQDLDDQTLAVSIVGDKGVGYIYESEVSDDEDSSTEACKEINTLSNQLDELYLSDDSISSATKKTKKRRTMSVNSIYTDREEIDSEFEDEDFEKEGIATVERAMENNHDLDTALLELNTLRMSMNVTYHEVRIATITALLRRVYHFIATQTLGPKDAVVKVFNQWGLLFKRQAFDEEEYIDLMNIIMEKIVEQSFDKPDLILFSALVSLYDNDIIEEDVIYKWWDNVSTDPRYDEVKKLTVKWVEWLQNADEESSSEEE EFE87F6AE2941619 ei2ba_yeast Translation initiation factor eIF-2B subunit alpha GCN3 AAS2 eIF-2B GDP-GTP exchange factor subunit alpha GCD complex subunit GCN3 Guanine nucleotide exchange factor subunit GCN3 TIF221 YKR026C Translational activator GCN3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEFNITETYLRFLEEDTEMTMPIAAIEALVTLLRIKTPETAAEMINTIKSSTEELIKSIPNSVSLRAGCDIFMRFVLRNLHLYGDWENCKQHLIENGQLFVSRAKKSRNKIAEIGVDFIADDDIILVHGYSRAVFSLLNHAANKFIRFRCVVTESRPSKQGNQLYTLLEQKGIPVTLIVDSAVGAVIDKVDKVFVGAEGVAESGGIINLVGTYSVGVLAHNARKPFYVVTESHKFVRMFPLSSDDLPMAGPPLDFTRRTDDLEDALRGPTIDYTAQEYITALITDLGVLTPSAVSEELIKMWYD 8E9A928D6D56E12B gcd11-sui2-9 1937470 N/A 1937474 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 1937475 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 1937476 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 1.0 physical association physical association false false false Fig. 1A gcd11-sui2-10 1937470 N/A 1937475 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 1937476 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 1.0 physical association physical association false false false Fig. 1A gcd1-gcd2-2 1937470 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937478 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937479 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937481 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 physical association physical association false false false Fig. 1B, Supp. Fig. 1 gcd1-gcd2-4 1937470 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937478 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937479 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937481 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 physical association physical association false false false Fig. 1B, Supp. Fig. 1 gcd1-gcd2-5 1937470 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937478 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937479 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 physical association physical association false false false Fig. 2A, 2B, Supp. Figs. 1 and 2 gcd1-gcd6-3 1937470 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 physical association physical association false false false Fig. 2 B/C gcd1-gcd2-6 1937470 N/A 1937478 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937479 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 physical association physical association false false false Supp. Fig 2 gcd1-gcd2-7 1937470 N/A 1937479 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937478 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 direct interaction direct interaction false false false Supp. Fig. 2 gcd1-gcd2-8 1937470 N/A 1937479 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 N/A 1937478 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 2 gcd1-gcd6-4 1937470 N/A 1937477 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 1.0 N/A 1937480 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 physical association physical association false false false Fig. 2A, 2B, 2C gcd1-gcd2-3 1937471 N/A 1937477 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1937478 unspecified role unspecified role prey prey N/A 1937479 unspecified role unspecified role prey prey N/A 1937480 unspecified role unspecified role prey prey N/A 1937481 unspecified role unspecified role prey prey association association false false false Fig. 1B gcd1-gcd11-2 1937471 N/A 1937475 unspecified role unspecified role prey prey ser-52 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false N/A 1937476 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1937474 unspecified role unspecified role prey prey N/A 1937477 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1937478 unspecified role unspecified role prey prey N/A 1937479 unspecified role unspecified role prey prey N/A 1937480 unspecified role unspecified role prey prey N/A 1937481 unspecified role unspecified role prey prey association association false false false Supp. Fig. 3A, 3B The eIF2 and eIF2B interact weakly. gcd1-gcd2-10 1937471 N/A 1937477 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 1937478 unspecified role unspecified role prey prey N/A 1937479 unspecified role unspecified role prey prey N/A 1937480 unspecified role unspecified role prey prey association association false false false Fig. 2A gcd1-gcd2-9 1937472 N/A 1937477 unspecified role unspecified role neutral component neutral component lys-412 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-494 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-412 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-376 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-410 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 1937478 unspecified role unspecified role neutral component neutral component lys-136 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-380 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-380 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-329 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-380 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 N/A 1937479 unspecified role unspecified role neutral component neutral component lys-648 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-95 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-570 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-76 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-422 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-562 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-570 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-575 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-579 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-580 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-564 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-421 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-133 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-556 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-559 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 N/A 1937480 unspecified role unspecified role neutral component neutral component lys-9 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-4 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-356 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-517 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-519 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-4 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 N/A 1937481 unspecified role unspecified role neutral component neutral component lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 1937596 1937576 1937599 1937593 1937600 1937594 1937601 1937595 1937602 1937583 1937603 1937577 1937604 1937578 1937605 1937579 1937606 1937580 1937607 1937581 1937608 1937582 1937611 1937584 1937613 1937589 1937615 1937597 1937616 1937609 1937617 1937610 1937618 1937585 1937619 1937586 1937620 1937587 1937621 1937588 1937622 1937612 1937624 1937575 physical association physical association false false false Supp. Table 3 gcd11-sui2-12 1937472 N/A 1937474 unspecified role unspecified role neutral component neutral component lys-170 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-214 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-272 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-281 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-260 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-102 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937475 unspecified role unspecified role neutral component neutral component lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-80 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937476 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lys-325 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-129 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-165 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-268 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-449 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-452 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-401 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-268 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-129 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-165 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false 1937637 1937627 1937638 1937628 1937639 1937629 1937640 1937630 1937648 1937641 1937649 1937642 1937650 1937643 1937651 1937645 1937652 1937631 1937653 1937644 1937654 1937632 1937655 1937633 1937656 1937634 1937657 1937635 physical association physical association false false false Supp. Table 4 gcd1-gcd11-3 1937472 N/A 1937475 unspecified role unspecified role prey prey lys-67 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-175 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937476 unspecified role unspecified role prey prey lys-66 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-113 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-518 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937474 unspecified role unspecified role prey prey lys-170 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-240 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-260 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937477 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937478 unspecified role unspecified role prey prey N/A 1937479 unspecified role unspecified role prey prey lys-365 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-422 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937480 unspecified role unspecified role prey prey lys-691 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-17 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-154 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 1937481 unspecified role unspecified role prey prey lys-145 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false 1937676 1937668 1937677 1937670 1937678 1937663 1937679 1937664 1937682 1937661 1937683 1937672 1937684 1937665 1937686 1937660 1937687 1937669 1937688 1937671 1937690 1937667 physical association physical association false false false Supp. Table 5 Amino acid 53 is not a lysine in GCD11 so this crosslink has not been included gcd11-sui2-11 1937473 N/A 1937474 unspecified role unspecified role prey prey N/A 1937475 unspecified role unspecified role prey prey N/A 1937476 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false Fig. 1A