IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gingras-2012-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions gingras-2012-2 in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. Bound proteins were analyzed by immunoblotting or Coomassie blue staining. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions gingras-2012-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions gingras-2012-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions gingras-2012-5 in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. An affinity matrix with an immobilized synthetic HEG1 C-terminal 19-residue peptide or HEG1 deletion mutant lacking the two C-terminal aas was used. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions gingras-2012-6 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions gingras-2012-7 in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH., Gingras AR., Liu JJ., Ginsberg MH. Bound proteins were analyzed by immunoblotting or Coomassie blue staining. KRIT1 FERM domain numbering found in PMID: 17954608, as were details of constructs used. ude.dscu@grebsnighm The Journal of cell biology 2012 imex curation Only protein-protein interactions krit1_human Krev interaction trapped protein 1 KRIT1 CCM1 Cerebral cavernous malformations 1 protein protein protein human Homo sapiens Human MGNPENIEDAYVAVIRPKNTASLNSREYRAKSYEILLHEVPIEGQKKKRKKVLLETKLQGNSEITQGILDYVVETTKPISPANQGIRGKRVVLMKKFPLDGEKMGREASLFIVPSVVKDNTKYTYTPGCPIFYCLQDIMRVCSESSTHFATLTARMLIALDKWLDERHAQSHFIPALFRPSPLERIKTNVINPAYATESGQTENSLHMGYSALEIKSKMLALEKADTCIYNPLFGSDLQYTNRVDKVVINPYFGLGAPDYSKIQIPKQEKWQRSMSSVTEDKERQWVDDFPLHRSACEGDSELLSRLLSERFSVNQLDSDHWAPIHYACWYGKVEATRILLEKGKCNPNLLNGQLSSPLHFAAGGGHAEIVQILLNHPETDRHITDQQGRSPLNICEENKQNNWEEAAKLLKEAINKPYEKVRIYRMDGSYRSVELKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENLSLQLKPYHKPLQHVRDWPEILAELTNLDPQRETPQLFLRRDVRLPLEVEKQIEDPLAILILFDEARYNLLKGFYTAPDAKLITLASLLLQIVYGNYESKKHKQGFLNEENLKSIVPVTKLKSKAPHWTNRILHEYKNLSTSEGVSKEMHHLQRMFLQNCWEIPTYGAAFFTGQIFTKASPSNHKVIPVYVGVNIKGLHLLNMETKALLISLKYGCFMWQLGDTDTCFQIHSMENKMSFIVHTKQAGLVVKLLMKLNGQLMPTERNS heg1_human Protein HEG homolog 1 HEG1 KIAA1237 protein protein human Homo sapiens Human MASPRASRWPPPLLLLLLPLLLLPPAAPGTRDPPPSPARRALSLAPLAGAGLELQLERRPEREPPPTPPRERRGPATPGPSYRAPEPGAATQRGPSGRAPRGGSADAAWKHWPESNTEAHVENITFYQNQEDFSTVSSKEGVMVQTSGKSHAASDAPENLTLLAETADARGRSGSSSRTNFTILPVGYSLEIATALTSQSGNLASESLHLPSSSSEFDERIAAFQTKSGTASEMGTERAMGLSEEWTVHSQEATTSAWSPSFLPALEMGELTTPSRKRNSSGPDLSWLHFYRTAASSPLLDLSSSSESTEKLNNSTGLQSSSVSQTKTMHVATVFTDGGPRTLRSLTVSLGPVSKTEGFPKDSRIATTSSSVLLSPSAVESRRNSRVTGNPGDEEFIEPSTENEFGLTSLRWQNDSPTFGEHQLASSSEVQNGSPMSQTETVSRSVAPMRGGEITAHWLLTNSTTSADVTGSSASYPEGVNASVLTQFSDSTVQSGGSHTALGDRSYSESSSTSSSESLNSSAPRGERSIAGISYGQVRGTAIEQRTSSDHTDHTYLSSTFTKGERALLSITDNSSSSDIVESSTSYIKISNSSHSEYSSFFHAQTERSNISSYDGEYAQPSTESPVLHTSNLPSYTPTINMPNTSVVLDTDAEFVSDSSSSSSSSSSSSSSGPPLPLPSVSQSHHLFSSILPSTRASVHLLKSTSDASTPWSSSPSPLPVSLTTSTSAPLSVSQTTLPQSSSTPVLPRARETPVTSFQTSTMTSFMTMLHSSQTADLKSQSTPHQEKVITESKSPSLVSLPTESTKAVTTNSPLPPSLTESSTEQTLPATSTNLAQMSPTFTTTILKTSQPLMTTPGTLSSTASLVTGPIAVQTTAGKQLSLTHPEILVPQISTEGGISTERNRVIVDATTGLIPLTSVPTSAKEMTTKLGVTAEYSPASRSLGTSPSPQTTVVSTAEDLAPKSATFAVQSSTQSPTTVSSSASVNSCAVNPCLHNGECVADNTSRGYHCRCPPSWQGDDCSVDVNECLSNPCPSTAMCNNTQGSFICKCPVGYQLEKGICNLVRTFVTEFKLKRTFLNTTVEKHSDLQEVENEITKTLNMCFSALPSYIRSTVHASRESNAVVISLQTTFSLASNVTLFDLADRMQKCVNSCKSSAEVCQLLGSQRRIFRAGSLCKRKSPECDKDTSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLLILGIALIVTCCRKNKNDISKLIFKSGDFQMSPYAEYPKNPRSQEWGREAIEMHENGSTKNLLQMTDVYYSPTSVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF rap1a_mouse Ras-related protein Rap-1A Rap1a Krev-1 Ras-related protein Krev-1 protein protein mouse Mus musculus Mouse MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL ccm2_human Cerebral cavernous malformations 2 protein CCM2 C7orf22 Malcavernin PP10187 protein protein human Homo sapiens Human MEEEGKKGKKPGIVSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLPERVEPDRLLSDYIEKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATELLQDYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTTNGNRATGSSDDRSAPSEGDEWDRMISDISSDIEALGCSMDQDSA krit1-heg1-1 2867627 N/A 2867634 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ferm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2867635 unspecified role unspecified role prey prey cytoplasmic_tail sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) 2867643 2867641 physical association physical association false false false 1A krit1-rap1a-1 2867628 N/A 2867636 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P62835:p.Gly12Val mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 2867634 unspecified role unspecified role bait bait O00522:p.Arg452Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O00522:p.[Leu717Ala;Leu721Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ferm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 4D heg1-krit1-1 2867629 N/A 2867634 unspecified role unspecified role neutral component neutral component possible_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ferm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2867635 unspecified role unspecified role neutral component neutral component cytoplasmic_tail sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 2867659 2867657 direct interaction direct interaction false false false 2867629 1C heg1-krit1-2 2867630 N/A 2867634 unspecified role unspecified role neutral component neutral component ferm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2867635 unspecified role unspecified role neutral component neutral component cytoplasmic_tail sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Table 2 2.49A RWork =0.234 RFree =0.309 heg1-krit1-3 2867631 N/A 2867634 unspecified role unspecified role prey prey O00522:p.[Leu717Ala;Leu721Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ferm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2867635 unspecified role unspecified role bait bait cytoplasmic_tail sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9ULI3:p.Tyr1380_Phe1381del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 4 A/B heg1-krit1-4 2867632 N/A 2867634 unspecified role unspecified role bait bait O00522:p.[Leu717Ala;Leu721Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2867637 unspecified role unspecified role prey prey n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4C heg1-krit1-5 2867633 N/A 2867634 unspecified role unspecified role bait bait n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ferm_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2867635 unspecified role unspecified role prey prey cytoplasmic_tail sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cytoplasmic_tail binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9ULI3:p.[Asn1367Ala;Phe1370Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 2867685 2867683 physical association physical association false false false 1B