UniProt UniProt http://www.uniprot.org balasubramaniyan-2012-1 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. Only protein-protein interactions balasubramaniyan-2012-2 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. Only protein-protein interactions balasubramaniyan-2012-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. Only protein-protein interactions balasubramaniyan-2012-4 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. Only protein-protein interactions balasubramaniyan-2012-5 human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. Only protein-protein interactions setd7_human Histone-lysine N-methyltransferase SETD7 SETD7 KIAA1717 SET7 KMT7 SET9 Histone H3-K4 methyltransferase SETD7 SET domain-containing protein 7 SET7/9 Lysine N-methyltransferase 7 protein protein human Homo sapiens Human MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK nr1h4_human Bile acid receptor NR1H4 BAR FXR HRR1 RIP14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 protein protein human Homo sapiens Human MVMQFQGLENPIQISPHCSCTPSGFFMEMMSMKPAKGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ nr1h4-setd7-1 1894919 N/A 1894924 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1894925 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q96RI1:p.Lys218Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false Q96RI1:p.Lys220Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 6c nr1h4-setd7-2 1894920 N/A 1894924 unspecified role unspecified role prey prey N/A 1894925 unspecified role unspecified role bait bait physical association physical association false false false 8a nr1h4-setd7-3 1894921 N/A 1894924 unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes N/A 1894925 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q96RI1:p.Lys220Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 8c nr1h4-setd7-4 1894922 N/A 1894924 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection N/A 1894925 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection Q96RI1:p.Lys220Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 9 GW 4064 (synthetic FXR agonist) setd7-nr1h4-1 1894923 N/A 1894924 unspecified role unspecified role bait bait N/A 1894925 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 8b