NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home schulz-2012-7 mouse-pro b cells Mus musculus pro-b-cell pro b cell ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Schulz D., Vassen L., Chow KT., McWhirter SM., Amin RH., Möröy T., Schlissel MS. mark_schlissel@brown.edu The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation gfi1b_mouse Zinc finger protein Gfi-1b Gfi1b Growth factor independent protein 1B protein protein mouse Mus musculus Mouse MPRSFLVKSKKAHTYHQPRAQGDELVWPPAVIPVAKEHSQSASPLLSTPLPSQTLDWNTIKQEREMLLNQSLPKMASAPEGPLVTPQPQDGESPLSESPPFYKPSFSWDTLASSYSHSYTQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFRLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDVCGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK rag1_mouse_gene V(D)J recombination-activating protein 1 Rag1 gene gene mouse Mus musculus Mouse rag2_mouse_gene Rag2 gene gene gene mouse Mus musculus Mouse foxo1_mouse_gene Forkhead box protein O1 Foxo1 Fkhr Foxo1a gene gene mouse Mus musculus Mouse gfi1_mouse Zinc finger protein Gfi-1 Gfi1 Gfi-1 Growth factor independent protein 1 protein protein mouse Mus musculus Mouse MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPAPGRAEGGAVSAGESKMEPRERLSPDSQLTEAPDRASASPNSCEGSVCDPCSEFEDFWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGSEPGRPAARYGPEQAAGGAGAGQPGRCGVAGGATSAAGLGLYGDFAPAAAGLYERPSTAAGRLYQDHGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK gfi1b-rag1-1 1934144 N/A 1934145 unspecified role unspecified role bait bait flag-tagged-gfi1b Flag-tagged-Gfi1b flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1934146 unspecified role unspecified role prey prey regulatory_region_rag1 Regulatory region Rag1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 8B O70237 decreases expression at RNA level of ENSMUSG00000061311 O70237 decreases the expression at RNA level of ENSMUSG00000061311 as ascertained by quantitative real-time PCR after overexpression of O70237 in AMuLV cells (Figure 1C). gfi1b-rag1-2 1934144 N/A 1934145 unspecified role unspecified role bait bait flag-tagged-gfi1b Flag-tagged-Gfi1b flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1934147 unspecified role unspecified role prey prey regulatory_region_rag2 Regulatory region Rag2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 8B O70237 decreases expression at RNA level of ENSMUSG00000032864 O70237 decreases the expression at RNA level of ENSMUSG00000032864 as ascertained by quantitative real-time PCR after overexpression of O70237 in AMuLV cells (Figure 1C). gfi1b-foxo1_mouse_gene-1 1934144 N/A 1934145 unspecified role unspecified role bait bait flag-tagged-gfi1b Flag-tagged-Gfi1b flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1934148 unspecified role unspecified role prey prey foxo1-regulatory-region Foxo1 regulatory region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 10F O70237 decreases expression at RNA level of ENSMUSG00000032864 O70237 decreases expression at RNA level of ENSMUSG00000044167 O70237 decreases the expression at RNA level of ENSMUSG00000044167 as ascertained by quantitative real-time PCR after overexpression of O70237 in AMuLV cells (Figure 10B). gfi1-rag1-3 1934144 N/A 1934149 unspecified role unspecified role bait bait flag-tagged-gfi1 Flag-tagged-Gfi1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1934146 unspecified role unspecified role prey prey regulatory_region_rag1 Regulatory region Rag1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 8B gfi1-rag2-1 1934144 N/A 1934149 unspecified role unspecified role bait bait flag-tagged-gfi1 Flag-tagged-Gfi1 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1934147 unspecified role unspecified role prey prey regulatory_region_rag2 Regulatory region Rag2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 8B