NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home wang-2008-8 in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift antibody detection identification by antibody antibody detection Wang J., Bian C., Li J., Couch FJ., Wu K., Zhao RC., Wang J., Bian C., Li J., Couch FJ., Wu K., Zhao RC. chunhuaz@public.tpt.tj.cn The Journal of biological chemistry 2008 imex curation wang-2008-11 in vitro In vitro ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Wang J., Bian C., Li J., Couch FJ., Wu K., Zhao RC., Wang J., Bian C., Li J., Couch FJ., Wu K., Zhao RC. chunhuaz@public.tpt.tj.cn The Journal of biological chemistry 2008 imex curation wang-2008-9 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Wang J., Bian C., Li J., Couch FJ., Wu K., Zhao RC., Wang J., Bian C., Li J., Couch FJ., Wu K., Zhao RC. chunhuaz@public.tpt.tj.cn The Journal of biological chemistry 2008 imex curation brca2_human_gene Breast cancer type 2 susceptibility protein BRCA2 FACD FANCD1 gene gene human Homo sapiens Human parp1_human Poly [ADP-ribose] polymerase 1 PARP1 ADPRT PPOL NAD(+) ADP-ribosyltransferase 1 Poly[ADP-ribose] synthase 1 ADP-ribosyltransferase diphtheria toxin-like 1 DNA ADP-ribosyltransferase PARP1 Protein poly-ADP-ribosyltransferase PARP1 protein protein human Homo sapiens Human MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW parp1-brca2_gene_promoter_by_emsa_supershif-1 1928881 N/A 1928884 tag visualisation tag visualisation unspecified role unspecified role bait bait biotinylated_probe Biotinytaled probe biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false brca2_promoter_region Promoter region of BRCA2 sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1928885 tag visualisation tag visualisation unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association false false false Figure 2C P09874 decreases expression at RNA level of ENSG00000139618 P09874 decreases expression at RNA level of ENSG00000139618 as ascertained by luciferase reporter assay reported in Figure 2E and real-time PCR reported in Figure 4D. brca2-parp1-2 1928882 N/A 1928884 tag visualisation tag visualisation unspecified role unspecified role prey prey brca2_promoter_region Promoter region of BRCA2 sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1928885 tag visualisation tag visualisation unspecified role unspecified role bait bait association association false false false Figure 3 parp1-brca2-1 1928883 N/A 1928884 tag visualisation tag visualisation unspecified role unspecified role bait bait biotinylated_probe Biotinytaled probe biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false brca2_promoter_region Promoter region of BRCA2 sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 1928885 tag visualisation tag visualisation unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells direct interaction direct interaction false false false Figures 2A and 2B