UniProt UniProt http://www.uniprot.org roepman-2005-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of N-RPGR (Table 1; 3 and 4) according PMID 8817343 (following PMID 10800709 cited by Ref. 2 in supporting information (PMID 10958648)). Origin of RPGRIP1(RID) (Table 1; 3 and 4) according Ref. 2 in supporting information (PMID 10958648); however, the sequence length of bovine RPGRIP1 differs between UniProt and the publication. r.roepman@antrg.umcn.nl Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation roepman-2005-3 chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation roepman-2005-4 chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation roepman-2005-5 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation roepman-2005-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease Bovine RPGRIP1 binding site according alignment in supplemantary fig 6A and B r.roepman@antrg.umcn.nl bovine retina cDNA Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation q96kn7-1 X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 RPGRIP1 protein protein human Homo sapiens Human MSHLVDPTSGDLPVRDIDAIPLVLPASKGKNMKTQPPLSRMNREELEDSFFRLREDHMLVKELSWKQQDEIKRLRTTLLRLTAAGRDLRVAEEAAPLSETARRGQKAGWRQRLSMHQRPQMHRLQGHFHCVGPASPRRAQPRVQVGHRQLHTAGAPVPEKPKRGPRDRLSYTAPPSFKEHATNENRGEVASKPSELVSGSNSIISFSSVISMAKPIGLCMPNSAHIMASNTMQVEEPPKSPEKMWPKDENFEQRSSLECAQKAAELRASIKEKVELIRLKKLLHERNASLVMTKAQLTEVQEAYETLLQKNQGILSAAHEALLKQVNELRAELKEESKKAVSLKSQLEDVSILQMTLKEFQERVEDLEKERKLLNDNYDKLLESMLDSSDSSSQPHWSNELIAEQLQQQVSQLQDQLDAELEDKRKVLLELSREKAQNEDLKLEVTNILQKHKQEVELLQNAATISQPPDRQSEPATHPAVLQENTQIEPSEPKNQEEKKLSQVLNELQVSHAETTLELEKTRDMLILQRKINVCYQEELEAMMTKADNDNRDHKEKLERLTRLLDLKNNRIKQLEGILRSHDLPTSEQLKDVAYGTRPLSLCLETLPAHGDEDKVDISLLHQGENLFELHIHQAFLTSAALAQAGDTQPTTFCTYSFYDFETHCTPLSVGPQPLYDFTSQYVMETDSLFLHYLQEASARLDIHQAMASEHSTLAAGWICFDRVLETVEKVHGLATLIGAGGEEFGVLEYWMRLRFPIKPSLQACNKRKKAQVYLSTDVLGGRKAQEEEFRSESWEPQNELWIEITKCCGLRSRWLGTQPSPYAVYRFFTFSDHDTAIIPASNNPYFRDQARFPVLVTSDLDHYLRREALSIHVFDDEDLEPGSYLGRARVPLLPLAKNESIKGDFNLTDPAEKPNGSIQVQLDWKFPYIPPESFLKPEAQTKGKDTKDSSKISSEEEKASFPSQDQMASPEVPIEAGQYRSKRKPPHGGERKEKEHQVVSYSRRKHGKRIGVQGKNRMEYLSLNILNGNTPEQVNYTEWKFSETNSFIGDGFKNQHEEEEMTLSHSALKQKEPLHPVNDKESSEQGSEVSEAQTTDSDDVIVPPMSQKYPKADSEKMCIEIVSLAFYPEAEVMSDENIKQVYVEYKFYDLPLSETETPVSLRKPRAGEEIHFHFSKVIDLDPQEQQGRRRFLFDMLNGQDPDQGHLKFTVVSDPLDEEKKECEEVGYAYLQLWQILESGRDILEQELDIVSPEDLATPIGRLKVSLQAAAVLHAIYKEMTEDLFS o75161-1 Nephrocystin-4 Nephroretinin NPHP4 KIAA0673 protein protein human Homo sapiens Human MNDWHRIFTQNVLVPPHPQRARQPWKESTAFQCVLKWLDGPVIRQGVLEVLSEVECHLRVSFFDVTYRHFFGRTWKTTVKPTKRPPSRIVFNEPLYFHTSLNHPHIVAVVEVVAEGKKRDGSLQTLSCGFGILRIFSNQPDSPISASQDKRLRLYHGTPRALLHPLLQDPAEQNRHMTLIENCSLQYTLKPHPALEPAFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPITGHLDDLFFTLYPSLEKFEEELLELHVQDHFQEGCGPLDGGALEILERRLRVGVHNGLGFVQRPQVVVLVPEMDVALTRSASFSRKVVSSSKTSSGSQALVLRSRLRLPEMVGHPAFAVIFQLEYVFSSPAGVDGNAASVTSLSNLACMHMVRWAVWNPLLEADSGRVTLPLQGGIQPNPSHCLVYKVPSASMSSEEVKQVESGTLRFQFSLGSEEHLDAPTEPVSGPKVERRPSRKPPTSPSSPPAPVPRVLAAPQNSPVGPGLSISQLAASPRSPTQHCLARPTSQLPHGSQASPAQAQEFPLEAGISHLEADLSQTSLVLETSIAEQLQELPFTPLHAPIVVGTQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATEPVTFNPQKEESDCLQSNEMVLQFLAFSRVAQDCRGTSWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGFQLRYMVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQASHELEVVATEYEQDNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCSTLPPSRSRVISNDGASRFSGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDATRRRKLERMRSVRLQEAGGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLSLAITTEHTLHATLGVAEFFEFVLKNPHNTQHTVTVEIDNPELSVIVDSQEWRDFKGAAGLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFKFQSFSAGQLAMVQASPGLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQVFRFYHPELSFLKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVASGPSPEIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKVRAFTSHPQELKTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLISKAFEIMLAAGEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVGEEEILIYINDHEDKNEEAFCVKVIYQ rpgr1_bovin X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 RPGRIP1 protein protein bovin Bos taurus (Bovine) Bovine MIPTSKGKNTKTQPPLSRMTRDELEDSLFRLREEHMLVKELFWKQQDEIKRMRTALLRLTASGRGLRAEAAADESSGSPLNGGGTESGGTAPSSTSVPRCPGSSCSSSAWAPLLPAAPSLASTRDTDSSTPPGHRAEKPKRESRDRLSYTAPPTFKEHVTNEKARGEVASEPSELGHLMTSDTMQVEEPPKSPEKMWSKDENFAQRSSLESTQKATELRASIKENIQLIRLKKLLHERNTSLAVTKAQLTEVQAAYETLLHKNQGILGAAHNALLSQVNELRAELKEESKKAVSLKSQMEDVSILQITLKEFQERVEDLEKERKLLNDNYDKLLESMLDSSNQPQWSHELGEQLQQKVSQLQDQLDVEMKEKREILLQLSQEKAQNKDLELEVTSLLQKHKQEVEDLQNISTFSQSPDRQSAPATHPALFQETIQIQPCEPKNQEEKKLSQMLSELQVSHAETTLELEKTRDMLILQRKINVCYQEELEAMMTKADNENKDHEAKLERLNQLLDLKNKRINQLEEQLKDVAYGTRQLPLCLKPLPAHENEDKVDISPWHQSENLFELHIHQAFLTSAALAQAGDTQPTTFCTYSFYDFETHCTPLVVGPQPLYDFTSQYVVEIDSLFLHYLQGASAQLDLHQAIASEHHTLAAGWICFDRVLETVERVHGSATLTGTGGEVFGVLEYWMRLRFPIRSSLQAYNKRKKAQAYLAANVLGAWEAQKDEPRSGTWKNQNELRVEIIRCCGLRSRSLGAQPSPYVMYRFFTFSDHDTTIIPASSNPYFRDLARFPVLVTSDLDQYLRREALSVYVFDDEDSEPGSYLGRVQVPLLPLAQNKSIQGDFNLTDPVGEPNGSVQVHLDWGSCYLPPENFPKPEAQSEEDTRDGLETSIEEEEASFPPQDQMVSIDTPTEAGQYQAKRKPPQVGERKEREHQVAGYSRRKHGRKTGLQGKNRMEYLSHNLLNGNTLQQVKYIEWKFSGLKISADHVLKNQQKEEEMTSSYSAQILKETPHPVNDKEFCEQASEGSEAQTTDSDEIVTPVSQKCPKADSEKMCIEIVSLAFYPEAEVMCDENVEQVYVEYRFYDLPLSETETPVSLRKPRAGEEIYFHFSKVIDLDPVEQKERRQFLFTMLIGEDPEQGHLKFTVVSDPIEEEKKECQEVGYAYLELWPMLVSGRDILEQDLDIVGPEDQATPIGKLKVSLQAAAALQAIYKEMTEDLCS q92834-2 X-linked retinitis pigmentosa GTPase regulator RPGR RP3 XLRP3 protein protein human Homo sapiens Human MREPEELMPDSGAVFTFGKSKFAENNPGKFWFKNDVPVHLSCGDEHSAVVTGNNKLYMFGSNNWGQLGLGSKSAISKPTCVKALKPEKVKLAACGRNHTLVSTEGGNVYATGGNNEGQLGLGDTEERNTFHVISFFTSEHKIKQLSAGSNTSAALTEDGRLFMWGDNSEGQIGLKNVSNVCVPQQVTIGKPVSWISCGYYHSAFVTTDGELYVFGEPENGKLGLPNQLLGNHRTPQLVSEIPEKVIQVACGGEHTVVLTENAVYTFGLGQFGQLGLGTFLFETSEPKVIENIRDQTISYISCGENHTALITDIGLMYTFGDGRHGKLGLGLENFTNHFIPTLCSNFLRFIVKLVACGGCHMVVFAAPHRGVAKEIEFDEINDTCLSVATFLPYSSLTSGNVLQRTLSARMRRRERERSPDSFSMRRTLPPIEGTLGLSACFLPNSVFPRCSERNLQESVLSEQDLMQPEEPDYLLDEMTKEAEIDNSSTVESLGETTDILNMTHIMSLNSNEKSLKLSPVQKQKKQQTIGELTQDTALTENDDSDEYEEMSEMKEGKACKQHVSQGIFMTQPATTIEAFSDEEVEIPEEKEGAEDSKGNGIEEQEVEANEENVKVHGGRKEKTEILSDDLTDKAEDHEFSKTEELKLEDVDEEINAENVESKKKTVGDDESVPTGYHSKTEGAERTNDDSSAETIEKKEKANLEERAICEYNENPKGYMLDDADSSSLEILENSETTPSKDMKKTKKIFLFKRVPSINQKIVKNNNEPLPEIKSIGDQIILKSDNKDADQNHMSQNHQNIPPTNTERRSKSCTIL e1bjb5_bovin NPHP4 protein protein bovin Bos taurus (Bovine) Bovine MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ q68cz1-1 Protein fantom Nephrocystin-8 RPGR-interacting protein 1-like protein RPGRIP1L FTM KIAA1005 NPHP8 protein protein human Homo sapiens Human MSGPTDETAGDLPVKDTGLNLFGMGGLQETSTTRTMKSRQAVSRVSREELEDRFLRLHDENILLKQHARKQEDKIKRMATKLIRLVNDKKRYERVGGGPKRLGRDVEMEEMIEQLQEKVHELEKQNETLKNRLISAKQQLQTQGYRQTPYNNVQSRINTGRRKANENAGLQECPRKGIKFQDADVAETPHPMFTKYGNSLLEEARGEIRNLENVIQSQRGQIEELEHLAEILKTQLRRKENEIELSLLQLREQQATDQRSNIRDNVEMIKLHKQLVEKSNALSAMEGKFIQLQEKQRTLRISHDALMANGDELNMQLKEQRLKCCSLEKQLHSMKFSERRIEELQDRINDLEKERELLKENYDKLYDSAFSAAHEEQWKLKEQQLKVQIAQLETALKSDLTDKTEILDRLKTERDQNEKLVQENRELQLQYLEQKQQLDELKKRIKLYNQENDINADELSEALLLIKAQKEQKNGDLSFLVKVDSEINKDLERSMRELQATHAETVQELEKTRNMLIMQHKINKDYQMEVEAVTRKMENLQQDYELKVEQYVHLLDIRAARIHKLEAQLKDIAYGTKQYKFKPEIMPDDSVDEFDETIHLERGENLFEIHINKVTFSSEVLQASGDKEPVTFCTYAFYDFELQTTPVVRGLHPEYNFTSQYLVHVNDLFLQYIQKNTITLEVHQAYSTEYETIAACQLKFHEILEKSGRIFCTASLIGTKGDIPNFGTVEYWFRLRVPMDQAIRLYRERAKALGYITSNFKGPEHMQSLSQQAPKTAQLSSTDSTDGNLNELHITIRCCNHLQSRASHLQPHPYVVYKFFDFADHDTAIIPSSNDPQFDDHMYFPVPMNMDLDRYLKSESLSFYVFDDSDTQENIYIGKVNVPLISLAHDRCISGIFELTDHQKHPAGTIHVILKWKFAYLPPSGSITTEDLGNFIRSEEPEVVQRLPPASSVSTLVLAPRPKPRQRLTPVDKKVSFVDIMPHQSDETSPPPEDRKEISPEVEHIPEIEINMLTVPHVPKVSQEGSVDEVKENTEKMQQGKDDVSLLSEGQLAEQSLASSEDETEITEDLEPEVEEDMSASDSDDCIIPGPISKNIKQSLALSPGLGCSSAISAHCNFRLPGSSDFPASASQVDGITGACHHTQPSEKIRIEIIALSLNDSQVTMDDTIQRLFVECRFYSLPAEETPVSLPKPKSGQWVYYNYSNVIYVDKENNKAKRDILKAILQKQEMPNRSLRFTVVSDPPEDEQDLECEDIGVAHVDLADMFQEGRDLIEQNIDVFDARADGEGIGKLRVTVEALHALQSVYKQYRDDLEA nphp4-rpgrip1-1 2843417 N/A 2843422 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843423 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2843432 2843430 physical association physical association false false false Table 1; 38-40 rpgr-rpgrip1-5 2843417 N/A 2843424 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false N/A 2843425 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2843437 2843435 physical association physical association false false false Table 1; 3 rpgr-rpgrip1-6 2843417 N/A 2843424 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false N/A 2843425 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2843442 2843440 physical association physical association false false false Table 1; 4 rpgrip1-nphp4_bovin-1 2843417 N/A 2843426 unspecified role unspecified role prey prey N/A 2843424 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1; 19 and 20 rpgrip1-nphp4-1 2843417 N/A 2843423 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843424 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false 2843451 2843449 physical association physical association false false false Table 1; 27 nphp4-rpgrip1-2 2843417 N/A 2843422 unspecified role unspecified role prey prey Q96KN7-1:p.Arg852Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q96KN7-1:p.Gly746Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q96KN7-1:p.Asp876Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false mutation arg852gln has no significant effect on association N/A 2843423 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O75161-1:p.Gly754Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false mutations arg740his and arg848trp have no significant effect on association 2843460 2843455 physical association physical association false false false Table 1; 28 and Table 2; 8,9, 10, 12 and 13, fig. 5B and D rpgrip1-nphp4-2 2843417 N/A 2843423 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843424 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false 2843467 2843465 physical association physical association false false false Table 1; 29-31 nphp4-rpgrip1-3 2843417 N/A 2843423 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843427 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2843472 2843470 physical association physical association false false false Table 1; 48 rpgrip1l-nphp4-4 2843417 N/A 2843423 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843427 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1; 47 rpgrip1-nphp4-3 2843418 N/A 2843422 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843423 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2843484 2843481 physical association physical association false false false 2B and supplementary 9B nphp4-rpgrip1-4 2843418 N/A 2843422 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 2843423 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 2843491 2843488 physical association physical association false false false 2C and supplementary 9A rpgrip1-nphp4-7 2843418 N/A 2843422 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q96KN7-1:p.Asp876Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q96KN7-1:p.Arg852Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false mutation arg852gln has no effect on association N/A 2843423 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O75161-1:p.Gly754Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false mutant arg740his has no effect on association physical association physical association false false false 5E rpgrip1-nphp4-4 2843419 N/A 2843422 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus cyfp cYFP n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2843423 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 3H nphp4-rpgrip1-6 2843419 N/A 2843422 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2843423 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus cyfp cYFP n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false supplemental 10 H,L and P rpgrip1-nphp4-6 2843420 N/A 2843422 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2843423 unspecified role unspecified role prey prey cell lysate cell lysate n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 2843521 2843518 physical association physical association false false false supplemental 8B nphp4-rpgrip1-7 2843420 N/A 2843422 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 N/A 2843423 unspecified role unspecified role bait bait cell lysate cell lysate region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) 2843527 2843525 physical association physical association false false false supplemental 8D rpgrip1-1 2843421 n/a 2843426 unspecified role unspecified role prey prey N/A 2843424 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false no figure